+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

mRNA secondary structure as a determinant in cap recognition and initiation complex formation. ATP-Mg2+ independent cross-linking of cap binding proteins to m7I-capped inosine-substituted reovirus mRNA

, : mRNA secondary structure as a determinant in cap recognition and initiation complex formation. ATP-Mg2+ independent cross-linking of cap binding proteins to m7I-capped inosine-substituted reovirus mRNA. Journal of Biological Chemistry 258(2): 707-710

Polypeptides of Mr = 50,000 and 80,000 in rabbit reticulocyte initiation factor preparations can be specifically cross-linked to the oxidized t' cap structure of native reovirus mRNA in an ATP-Mg2+-dependent manner (Sonenberg, N., Guertin, D., Cleveland, D., and Trachsel, H. (1981) Cell 27, 563-572). However, specific cross-linking of these polypeptides can occur in the absence of ATP-Mg2+ when m7I-capped inosine substituted mRNA, which contains less secondary structure than native reovirus mRNA, is used. We also found, using wheat germ extract, that inhibition of initiation complex formation by high salt concentrations is directly related to the degree of secondary structure of the mRNA. Binding of ribosomes to bromouridine-substituted reovirus mRNA is severely inhibited at high K+ concentrations, while binding to inosine-substituted mRNA is only slightly inhibited and binding of native reovirus mRNA is inhibited to an intermediate degree. The results are consistent with the hypothesis that cap recognition factors mediate an ATP-dependent melting of secondary structures involving 5' proximal sequences to the initiation codon in order to facilitate binding of ribosomes during translation initiation.


PMID: 6600451

Other references

Bertolini, A.M.; Volta, A., 1953: Effect of 5, 5-diethyl-2-thiobarbituric acid on succinic dehydrogenase. Bollettino Della Societa Italiana di Biologia Sperimentale 29(1): 24-27

Chen, R.Y. (Chen, J.Y.; Song, W.Q. (Sung, W.C.; Chen, X. (Chen, H); Li, D.C. (Li, T.C.; Xu, Y.F. (Hsu, Y.F.; Fan, H.P.; Zhou, X.C. (Chou, H.C., 1979: Studies on the Giemsa plant chromosome banding technique. A modified Giemsa banding technique was used successfully in Secale cereale, Zea mays, Hordeum vulgare, Triticum aestivum, triticale and also in Vicia faba. The C-banding characteristics of these crops were analysed and the bands produced by the h...

Katsuta, H.; Kiryu, J.; Miyamoto, K.; Nishijima, K.; Miyahara, S.; Hirose, F.; Tamura, H.; Honda, Y., 2003: Proinsulin c peptide attenuates retinal ischemia reperfusion injury. Purpose: C-peptide consists of 31 amino acids and is derived from proinsulin when it is converted into insulin. Recently, its various functions have been reported, including inhibition of leukocyte-endothelial interactions. We reported that attenu...

Gao, J.; Gong, H.; Huang, X.; Zhang, R.; Ma, R.; Zhu, D., 2016: Multi-Level Assessment of Fracture Calluses in Rats Subjected to Low-Magnitude High-Frequency Vibration with Different Rest Periods. The aim of this study was to investigate the influences of low-magnitude high-frequency vibration (LMHFV) with different rest period regimes (vibrational loading per day [with or without the loading divided into bouts]; or vibrational loading for...

George C.R.P.; Slichter S.J.; Quadracci L.J.; Kenny G.M.; Dennis M.B.; Striker G.E.; Harker L.A., 1976: The hemostatic system in transplant rejection. Australian & New Zealand Journal of Medicine 6(1): 92

Kryzhanovskii, G.N., 1984: The stable pathological state and pathological system. Human Physiology 10(5): 373-381

Birger, M.; Assulin, Y.; Kalian, N.; Allish, J.; Grinspan, H.; Kotler, M., 2000: The relationship between violence and psychopathology. Harefuah 137(1-2): 42-45

Concina, E.; Indovina, D., 1958: The electrocardiographic field, as explored by surface & intrathoracic leads. Minerva Medica 49(103): 4861-4865

Wypasek, E.; Potaczek, D.P.; Stąpor, R.; Coucke, P.J.; D.B.cker, J.; D.P.epe, A.M.; Undas, A., 2015: First report of the genetic background of Marfan syndrome in Polish patients. Polskie Archiwum Medycyny Wewnetrznej 123(11): 646-647

Anon., 1970: Orchids in polyurethane foam. Methods of raising orchids in polyurethane foam are discussed, with special reference to the "foam nest" methods in which young plants root directly into balls of plastic foam containing an organic substrate such as TKS II.