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A meta-analysis of reverse transcriptase-polymerase chain reaction for tyrosinase mRNA as a marker for circulating tumor cells in cutaneous melanoma

, : A meta-analysis of reverse transcriptase-polymerase chain reaction for tyrosinase mRNA as a marker for circulating tumor cells in cutaneous melanoma. Archives of Dermatology 137(3): 325-330

To systematically review the use of reverse transcriptase-polymerase chain reaction (RT-PCR) for tyrosinase messenger RNA as a molecular serum marker for metastatic melanoma. Computerized searches (1966-1999) of the PubMed and MDConsult databases and a manual search of retrieved article references. Cohort studies containing test subjects and negative controls were reviewed. Three investigators independently screened abstracts for relevant studies and 2 investigators independently reviewed all eligible studies. Of 127 identified studies, 50 were reviewed in detail and 23 met all inclusion criteria. From these 23 studies, the PCR methods, the total number of patients, the number of control subjects, and the number of RT-PCR-positive patients per stage were analyzed. Results of RT-PCR for tyrosinase messenger RNA were positive in 18% (95% confidence interval [CI], 3%-22%) patients for stage I disease, 28% (95% CI, 23%-34%) for stage II disease, 19% (95% CI, 16%-21%) for stage I/II localized disease, 30% (95% CI, 26%-34%) for stage III disease, and 45% (95% CI, 41%-50%) for stage IV disease. Specificities were 100% in all but 1 study. Results of RT-PCR were positive in only 0.4% of healthy controls and patients with nonmelanoma cancer. The lack of data on the outcome of stage I, II, and III patients who were RT-PCR positive and the low prevalence of RT-PCR positivity in patients with known stage IV disease limit the applicability of this test at this time. Ongoing and future studies on a quantitative RT-PCR, amplification of multiple melanoma-associated antigens, and use of the test as a prognostic indicator might improve the utility of this molecular serologic tool.


PMID: 11255333

Other references

Mottram, V.H., 1916: Fatty infiltration of the cat's kidney. Journal of Physiology 50(6): 380-390

Ying, S.L., 1992: Determinants of fertility in Malaysia -- how much do we know?. Chinese and Indian fertility have continued to decline in the 1980s while Malay fertility has stabilized and even risen slightly. The objective of this literature review is to examine the literature on the role of socioeconomic determinants of fam...

Laszlo, J.A.; Silman, R.W., 1993: Cellular automata simulations of fungal growth on solid substrates. Growth of filamentous fungi on the surface of cereal grains is a critical aspect of solid substrate fermentation (SSF). Numerous mathematical models have been developed to describe various aspects of fungal growth in SSF. These models consider hyp...

Steyn, P., 1977: Albinistic lilac-breasted roller. Honeyguide.,89: 24-25,43,illust.

Hartenstein R.; Jaeckel R.; Kellhammer U.; Clemm C.; Mann K.; Mellin H.E.; Loehrs U.; Wilmanns W., 1984: Prognostic determinants in patients with nonseminomatous testicular cancer. Journal of Cancer Research & Clinical Oncology 107(SUPPL): S72

Richter, C.P., 1978: Evidence for existence of a yearly clock in surgically and self-blinded chipmunks. By use of simplified technique and constant environmental conditions, I have demonstrated the existence in the chipmunk of a yearly clock. In a blinded chipmunk the clock manifested itself by remarkably consistent changes in running activity, food...

Zhang, H.; Weglein, A.B., 2009: Direct nonlinear inversion of multiparameter 1D elastic media using the inverse scattering series. In the direct nonlinear inversion method and in algorithms for 1D elastic media, P-wave velocity, S-wave velocity, and density are depth dependent. "Direct nonlinear" means that the method uses explicit formulas that (1) input data and d...

Klebig, M.L.; Wall, M.D.; Potter, M.D.; Rowe, E.L.; Carpenter, D.A.; Rinchik, E.M., 2003: Mutations in the clathrin-assembly gene Picalm are responsible for the hematopoietic and iron metabolism abnormalities in fit1 mice. Recessive N-ethyl-N-nitrosourea (ENU)-induced mutations recovered at the fitness-1 (fit1) locus in mouse chromosome 7 cause hematopoietic abnormalities, growth retardation, and shortened life span, with varying severity of the defects in different...

Bertels, A., 1970: Los foraminiferos planctonicos de la cuenca CretacicoTerciaria en Patagonia septentrional (Argentina), con consideraciones sobre la estratigrafia de Fortin General Roca (Provincia de Rio Negro). Ameghiniana: 1-56,9 pis

Soprano, D.R.; Harnish, D.C.; Soprano, K.J.; Kochhar, D.M.; Jiang, H., 1993: Correlations of RAR isoforms and cellular retinoid-binding proteins mRNA levels with retinoid-induced teratogenesis. Retinoic acid (RA) plays an important role in normal embryogenesis; however, excessive doses are teratogenic. At present, the molecular mechanisms responsible for these effects of RA are not well understood. The action of retinoids are believed to...