+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

A new computerized method of reading sperm morphology (strict criteria) is as efficient as technician reading

, : A new computerized method of reading sperm morphology (strict criteria) is as efficient as technician reading. Fertility and Sterility 59(1): 202-209

To compare the ability of a computerized method of sperm morphology with the manually recorded method in predicting in vitro fertilization (IVF) results, to compare results obtained by both methods, and to determine the intraobservation variability. Forty-three stained semen slide preparations from two large level-three academic institutions' reproductive endocrinology units (IVF programs) were blindly evaluated, and the sperm were classified into normal and amorphous forms. Experiment 1: Twenty-one slide preparations from the Tygerberg gamete intrafallopian transfer program were manually evaluated; the fertilization rates for the groups with < 14% and > 14% normal sperm forms were 33.3% (15/45 oocytes) and 76.6% (46/60 oocytes), respectively. Corresponding fertilization rates with FERTECH were 46.8% (30/64) and 75.6% (31/41). Experiment 2: Twenty-two slide preparations from the Norfolk IVF program were evaluated. The manual method reported a fertilization rate in the group with < 14% normal forms of 27.4% (14/51 oocytes) compared with 90.0% (127/141 oocytes) in the group with > 14% normal forms. Corresponding figures for the FERTECH method were 33.9% (18/53) and 88.4% (123/139), respectively. Experiment 3: When the 43 slide preparations were blindly evaluated using both methods, 84% of the FERTECH evaluations correlated well with the manual method and FERTECH ability to diagnose the subfertile male (< 14% normal forms) was 95% (sensitivity). Experiment 4: A total of 16 different slides (8 per group) were randomly selected and analyzed five times (100 cells per reading) by the computerized method. The slides were obtained from men with normal sperm morphology of < 14% and > 14% as classified by the manual method. In the first group (< 14%) 97.5% (39/40) of the readings classified the sperm in the proper category, whereas in the second group (> 14%) 95% (38/40) of the cases were correctly identified. Using strict criteria for morphology evaluation, there is a positive and significant correlation between FERTECH evaluation and manual assessment. The reproducibility of the computerized method and the ability to distinguish between fertile and subfertile groups using those criteria are good.


PMID: 8419209

Other references

Cacovich, S.; Ciná, L.; Matteocci, F.; Divitini, G.; Midgley, P.A.; Di Carlo, A.; Ducati, C., 2017: Gold and iodine diffusion in large area perovskite solar cells under illumination. Operational stability is the main issue hindering the commercialisation of perovskite solar cells. Here, a long term light soaking test was performed on large area hybrid halide perovskite solar cells to investigate the morphological and chemical...

Franklin, C.; Jackson, D., 1983: Discharge decision-making in a medical ICU: characteristics of unexpected readmissions. To identify those patients most likely to be readmitted to a Medical ICU (MICU), we studied 512 MICU admissions during a 1-yr period. There were 36 readmissions within the same hospitalization in that interval. When ICU deaths and short-term drug...

Alonso, A., 2005: A new model for home care for COPD. A new model for home care of COPD patients is investigated, as a part of a coordinated provision model across levels of care. In the Spanish pilot of the e-Vital project, relevant vital signs for COPD are closely monitored and used for early detec...

Hillocks, R.J., 1992: Microbial contamination of the lint. Hillocks, R J [Editor] Cotton diseases : 263-273

Takahashi, S.; Yamashita, T.; Eto, Y.; Shibai, H.; Miyamoto, K.; Ogata, E., 1990: Inducible gene expression of activin A/erythroid differentiation factor in HL-60 cells. Treatment of HL-60 cells with 12-O-tetradecanoyl-phorbol 13-acetate (TPA) for 48 h induced expression of mRNA of beta A chain of activin A/erythroid differentiation factor. Under the same condition, interferon-gamma caused a slight increase in bet...

Laryea, M.; Mayatepek, E.L.ichsenring, M., 1994: Analysis of vegetable foods consumed in tropical Africa. Modern methods of plant analysis = Moderne Methoden der Pflanzenanalyse6(16): 169-184

Kamaya, A.; Vaithilingam, S.; Chung, B.I.; Oralkan, O.; Khuri-Yakub, B.T., 2014: Photoacoustic imaging of the bladder: a pilot study. Photoacoustic imaging is a promising new technology that combines tissue optical characteristics with ultrasound transmission and can potentially visualize tumor depth in bladder cancer. We imaged simulated tumors in 5 fresh porcine bladders with...

O'Dempsey, T.; Munslow, B., 2015: 'Mind the gap!' rethinking the role of health in the emergency and development divide. The 'Emergency Relief-Rehabilitation-Development' model is highly problematic, especially in fragile states which fluctuate in and out of crisis. Lack of definition as to what constitutes a humanitarian emergency and the absence of rules...

Siegfried, L.; Filka, J.; Puzová, H., 1995: Role of alpha-haemolysin in resistance of Escherichia coli strains to bactericidal action of human serum and polymorphonuclear leukocytes. Advances in Experimental Medicine and Biology 371a: 691-693

Skolnick, P.; Boje, K.; Miller, R.; Pennington, M.; Maccecchini, M.L., 1992: Noncompetitive inhibition of N-methyl-D-aspartate by conantokin-G: evidence for an allosteric interaction at polyamine sites. Conantokins T and G are polypeptide toxins present in snails of the genus Conus. These substances were recently reported to act as N-methyl-D-aspartate (NMDA) antagonists. In the present study, we examined the possible mechanisms producing this an...