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An evaluation of tyramide signal amplification and archived fixed and frozen tissue in microarray gene expression analysis


, : An evaluation of tyramide signal amplification and archived fixed and frozen tissue in microarray gene expression analysis. Nucleic Acids Research 30(2): E4-E4

Archival formalin-fixed, paraffin-embedded and ethanol-fixed tissues represent a potentially invaluable resource for gene expression analysis, as they are the most widely available material for studies of human disease. Little data are available evaluating whether RNA obtained from fixed (archival) tissues could produce reliable and reproducible microarray expression data. Here we compare the use of RNA isolated from human archival tissues fixed in ethanol and formalin to frozen tissue in cDNA microarray experiments. Since an additional factor that can limit the utility of archival tissue is the often small quantities available, we also evaluate the use of the tyramide signal amplification method (TSA), which allows the use of small amounts of RNA. Detailed analysis indicates that TSA provides a consistent and reproducible signal amplification method for cDNA microarray analysis, across both arrays and the genes tested. Analysis of this method also highlights the importance of performing non-linear channel normalization and dye switching. Furthermore, archived, fixed specimens can perform well, but not surprisingly, produce more variable results than frozen tissues. Consistent results are more easily obtainable using ethanol-fixed tissues, whereas formalin-fixed tissue does not typically provide a useful substrate for cDNA synthesis and labeling.

US$29.90

PMID: 11788730


Other references

Nicholls, J.G., 1971: Movement responses and originality. Perceptual and Motor Skills 33(3): 698-698

Vegh, P.; Janossy, T.; Erdos, E.; Petri, G.; Kovats, T.G., 1976: Partial specific unresponsiveness to 2nd set allo grafts following chronic rejection of 1st set allo grafts. Data are presented suggesting that, under certain circumstances, the rejection of a 1st set allograft may be accompanied by partial specific unresponsiveness (prolonged graft survival) instead of sensitization (accelerated rejection) to a 2nd set...

Horowitz, Norman H., 1998: T. H. Morgan at Caltech: a reminiscence. The writer reminisces about Thomas Hunt Morgan, former chairman of the Kerckhoff Laboratory of Biology at Caltech. He describes his first encounter with Morgan upon arriving at Caltech in 1936 as a graduate student, his assignment to Albert Tyler...

Chen Daizhao, 1997: Current aspects of cyclic carbonate stratigraphy. Yanxiang Gudili = Sedimentary Facies and Palaeogeography 17(1): 64-70

Watson, C.J., 1939: Further observations on the red pigments of pellagra urines. Further observations of the color reaction described by Beckh, Ellinger, and Spies as characterizing pellagra urines have provided additional evidence that the reaction is not due to porphyrin as they believed. The color reaction is caused by the...

Mascola, J.R.; Sambor, A.; Beaudry, K.; Santra, S.; Welcher, B.; Louder, M.K.; Vancott, T.C.; Huang, Y.; Chakrabarti, B.K.; Kong, W-Pui.; Yang, Z-Yong.; Xu, L.; Montefiori, D.C.; Nabel, G.J.; Letvin, N.L., 2004: Neutralizing antibodies elicited by immunization of monkeys with DNA plasmids and recombinant adenoviral vectors expressing human immunodeficiency virus type 1 proteins. Immunization with recombinant serotype 5 adenoviral (rAd5) vectors or a combination of DNA plasmid priming and rAd5 boosting is known to elicit potent immune responses. However, little data exist regarding these immunization strategies and the dev...

Anonymous, 1972: Gondwana and the geologist (Public lecture in Cape Town). Direction of research into the Gondwana problem, role of the geologist as a scientist and citizen.

Carter, D.B.; Weiner, G., 1965: Abstract services in the field of visual science. Journal of the American Optometric Association 36(10): 901-901

Strombeck, D.R.; Harrold, D., 1988: Anal sphincter pressure and the rectosphincteric reflex in the dog. Anal sphincter pressure (ASP) was measured in healthy conscious dogs, and the rectosphincteric reflex that causes inhibition of the internal sphincter was identified. Pressures were measured in 6 dogs by use of a perfused miniature 4-lumen cathete...

Conceição, F.; King, M.A.; Yeadon, M.R.; Lewis, M.G.C.; Forrester, S.E., 2013: An isovelocity dynamometer method to determine monoarticular and biarticular muscle parameters. This study aimed to determine whether subject-specific individual muscle models for the ankle plantar flexors could be obtained from single joint isometric and isovelocity maximum torque measurements in combination with a model of plantar flexion....