+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Characterization of a cysteine proteinase from Taenia crassiceps cysts

, : Characterization of a cysteine proteinase from Taenia crassiceps cysts. Molecular and Biochemical Parasitology 85(2): 243-253

Human neurocysticercosis, due to infestation of the central nervous system with Taenia cysts, is a common cause of neurologic disease in endemic areas and is being increasingly recognized in the United States. Previous studies have suggested that Taenia cysts bind host IgG via Fc-like receptors and that bound IgG is degraded by the parasite, perhaps as a source of nutrients or a means of immune evasion. We now demonstrate that IgG degradation is thiol dependent and is inhibited by the cysteine proteinase inhibitor, E-64. The cysteine proteinase activity from Taenia crassiceps cysts was purified 682-fold by acid extraction, gel filtration chromatography, and anion-exchange FPLC. The cysteine proteinase appeared as a 43 kDa band on silver-stained gels. Its isoelectric point was 5.27. The purified enzyme was inhibited by cysteine proteinase inhibitors and also by chloromethyl ketone inhibitors, but not significantly by other inhibitors of serine, aspartic, or metallo-proteinases. Substrate studies showed pronounced cleavage of Z-Phe-Arg-7-amino-4-trifluoromethylcoumarin (Z-Phe-Arg-AFC), but not of substrates with neutral or positively charged amino acids in the P2 position. Km for Z-Phe-Arg-AFC was 1.0 x 10(-7) M, Kcat 58 s-1, and Kcat/Km 5.8 x 10(8) mol-1s-1. Amino acid sequencing of the amino terminus revealed a single cysteine residue with surrounding residues suggestive suggestive of a cysteine proteinase active site. The sequence, however, did not contain the conserved active site associated with enzymes of known cysteine proteinase families. This cysteine proteinase may play an important role in the interaction of Taenia cysts and host immunoglobulin and is a potential target for antiparasitic chemotherapy.


PMID: 9106197

Other references

Harbert, F.; Sataloff, J.; Lerner, S.S., 1957: Effect of masking on induced conduction deafness. A.M.A. Archives of Otolaryngology 66(5): 584-587

Almendros G.; Dorado E., 1984: Study of type p humic acids distribution of green pigments in the various human fractions of the soil. A sequential extraction of the organic matter in 2 soils containing P-type humic acids was done. Six fractions of humic acids were isolated from each soil. The stronger the linkage between soil and humic acids, the higher the percentage of green f...

Berggren, B.; Rooth, C.; Szecsoedy, I., 1963: Day-Care In The Psychiatric Department Of A General Hospital. Acta Psychiatrica Scandinavica 39: Suppl169:284-Suppl169:284

Krapivin, S.V.; Sopyev, Z.A., 1993: The effect of preparations with nootropic action when used long-term on the brain bioelectrical activity in rats. The impact of prolonged injection of piracetam (2 months), meclophenoxate (5 months), and mexidole (5 months) on the bioelectrical activity of the sensomotor cortex and dorsal hippocamp was studied in rats who behaved freely. The injects increased...

Anonymous, 2013: Uji Efektivitas Rizobakteri sebagai Agen Antagonis terhadap Fusarium oxysporum fsp capsici Penyebab Penyakit Layu Fusarium pada Tanaman Cabai Rawit Capsicum frutescens L. Effectiveness Test of Rhizobacteria as Antagonist Agents against Fusarium oxysporum f.sp. capsici Causes Fusarium Wilt Disease in Chili Pepper A Plants(Capsicum frutescens L.)Utilization of rhizobacteria as antagonist agents Fusarium oxysporum f.s...

Baxter, C.R.; Maynard, D.R., 1968: Prevention and recognition of surgical renal complications. Clinical Anesthesia 3: 322-333

Somayaji, B.N., 1970: Functional and metabolic effects of vagotomy and pyloroplasty. Quarterly Journal of Medicine 39(155): 441-460

Andrade, J.M.; Little, J.M.; Burnier, M.N.Jr, 1995: Ocular toxoplasmosis in children Are all congenital?. Investigative Ophthalmology & Visual Science 36(4): S1006

Kalac, P.; Novak, J., 1976: Preservatives for ensiling fodder crops. Silage additives were classified into chemical preservatives (strong mineral acids and their salts; sulphates; carboxylic acids such as formic, propionic and benzoic acids and their derivatives; formaldehyde; and other chemical substances, e.g. so...

Polyak, B.G.; Dubinina, E.O.; Lavrushin, V.Y.; Cheshko, A.L., 2008: Isotopic composition of thermal waters in Chukotka. Twenty three groups of thermomineral springs in eastern Chukotka with the discharge temperature of 2 to 97 degrees C and mineralization of 1.47 to 37.14 g/1 are studied and compared with surface freshwater from their localities. The delta D and de...