geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Characterization of the single-strand-specific BPV-1 origin binding protein, SPSF I, as the HeLa Pur alpha factor


, : Characterization of the single-strand-specific BPV-1 origin binding protein, SPSF I, as the HeLa Pur alpha factor. Nucleic Acids Research 24(14): 2799-2806

SPSF I and II are two cellular proteins which bind specifically to single-stranded DNA. SPSF I and II binding sites are found in the minimal origin of replication of BPV-1 DNA and near the P2 promoter of the cellular c-myc gene. DNA-binding properties of the two proteins to single-stranded oligonucleotides of different lengths and sequences were quantified by determination of DNA-binding constants. The binding constant of SPSF proteins to the lower strand of the BPV-1 origin was determined to be 1.5 x 10(-10) M-1. Peptide sequences derived from purified SPSF I and II revealed the identity of at least one of the SPSF proteins with the so-called HeLa Pur alpha factor. The HeLa Pur alpha factor was identified previously by virtue of its capacity to bind to purine-rich strands of the PUR element found in initiation zones of DNA replication [Bergemann, A.D., Ma,Z.-W. and Johnson, E.M. (1992) Mol. Cell. Biol. 12, 5673-5682]. Expression of the Pur cDNA confirmed the identity of the Pur alpha protein with the 42 kDa SPSF I protein. Analysis of several Pur alpha cDNA clones revealed the existence of an extended 3'-untranslated region in all Pur mRNAs.

US$29.90

PMID: 8759014


Other references

Hartmair, V., 1970: Can the fertility of grape buds be predetermined?. Winzer 26 (3) 46

Lie, W.N., 1995: Automatic target segmentation by locally adaptive image thresholding. A locally adaptive thresholding algorithm, concerning the extraction of targets from a given field of background, is proposed. Conventional histogram-based or global-type methods are deficient in detecting small targets of possibly low contrast as...

Ray, K.; Ramesh, V.; Karmakar, S.N.; Misra, R.S., 1996: Increasing trend of HIV seropositivity in a sexually transmitted diseases centre and epidemiology of HIV seropositive individuals. Although India's Sexually Transmitted Diseases (STD) Control Program has been in existence for 40 years, it was not until the acquired immunodeficiency syndrome (AIDS) epidemic that a serious attempt was made to strengthen the program and col...

Freitas, D.R.J.; Rosa, R.M.; Moraes, J.; Campos, E.; Logullo, C.; D.S.lva Vaz, I.; Masuda, A., 2006: Relationship between glutathione S-transferase, catalase, oxygen consumption, lipid peroxidation and oxidative stress in eggs and larvae of Boophilus microplus (Acarina: Ixodidae). Glutathione S-transferases (GSTs) are enzymes that act in excretion of physiologic and xenobiotic substances, protecting cells against chemical toxicity and stress. In this work, we characterized the enzymatic activity of GST in eggs and larvae o...

Matzinger, O.; Schneiter, P.; Tappy, L., 2002: Effects of fatty acids on exercise plus insulin-induced glucose utilization in trained and sedentary subjects. Fatty acids are known to decrease insulin-mediated glucose utilization in humans, both at rest and during exercise. To evaluate the effect of endurance training in this process, we infused lipids or saline in groups of sedentary and highly trained...

Schultze Motel, Jurgen, 1966: Gymnospermous woods from the Upper Cretaceous Umzamba layers of East-Pondoland. Woods belonging to the Araucariaceae and Podocarpaceae are classified as DAMMAROXYLON and Podocarpoxvlon Gothan. Fossil Araucariaceae have been deter-mined for the 1st time with certainty, on the basis of wood anatomy characters, and separated fro...

Brooks R.P.Jr., 1972: Double Creek (Smackover) field, Clarke County. Pages 31-32 1972

Crovitz, H.F.; Diaco, D.S.; Apter, A., 1992: Consistency in recalling features of former head injuries retrospective questionnaire vs. interview retest. The probability and features of memory concerning former head injuries with loss of consciouness was assessed in two formats: a group-test questionnaire and a retest using personal individual interview. Results are given concerning the greater lik...

Shelepina, G.A., 1971: Study of chromosome constitutions in water melons. Differences between Citrullus lanatus ssp. vulgaris (2n = 22) and Praecitrullsu fistulosus (2n = 24) in the size and structure of the chromosomes are outlined and illustrated.

Glavits, R.; Palfi, V.; Toth, A., 2005: Koleszteringranuloma (un. plexus cholesteatoma) elofordulasa loban - Esetismertetes. The authors diagnosed cholesterol granulomas (plexus cholesteatomas) in the plexus of lateral ventricle in an 8 years old Arabian mare, growing symmetrically and causing frequent and sever neurological signs.Condition of mare had worsened for mont...