geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Chemokine expression in Porphyromonas gingivalis-specific T-cell lines


, : Chemokine expression in Porphyromonas gingivalis-specific T-cell lines. Oral Microbiology and Immunology 15(3): 166-171

Autologous non-T cells (monocytes and B cells) were added to Porphyromonas gingivalis-specific T cell lines established from 9 healthy adults together with P. gingivalis outer membrane antigens for 4-6, 16-18, 24 and 48 h. Flow cytometry was employed to analyze the CD4 and CD8 cells, monocytes and B cells for intracytoplasmic IP-10 (interferon-gamma inducible protein 10), MCP-1 (monocyte chemoattractant protein 1), MIP-1 alpha (macrophage inflammatory protein 1 alpha) and RANTES (regulated on activation normal T cell expressed and secreted) at the four time periods. All cell types were positive for each chemokine throughout the 48-h time period. There were significantly fewer MCP-1-positive cells compared with the other 3 chemokines. However, the percentages of MCP-1, MIP-1 alpha- and RANTES-positive CD8 cells were significantly higher than the percentages of positive CD4 cells in all cultures. IP-10-positive CD4, CD14-positive monocytes and CD19-positive B cells were predominant compared with MIP-1 alpha- and RANTES-positive cells at 24 h. In conclusion, the present study has shown that P. gingivalis-specific T cells, monocytes and B cells produce chemokines in response to P. gingivalis outer membrane antigens, IP-10 being predominant, with MCP-1 being significantly reduced in comparison with IP-10, MIP-1 alpha and RANTES. Increased percentages of CD8 cells were induced to produce chemokines in comparison with CD4 cells, indicating a more preferential action on CD8 rather than CD4 cells.

US$29.90

PMID: 11154399


Other references

Neri, I.; Dante, G.; Pignatti, L.; Salvioli, C.; Facchinetti, F., 2017: Castor oil for induction of labour: a retrospective study. The aim of this study is to investigate the safety and efficacy of castor oil to induce labour. A retrospective observational case control study was conducted over five years. Castor oil was proposed to women referred to the Birth Centre (Castor O...

Ozol, A.M., 1950: Producing bush forms of Schizandra chinensis by cultural methods.. Schizandra chinensis is an ornamental shrub and a source of essential oils; its berries contain citric and malic acids and are used in confectionery. Its ecology is described. It is naturally a liane of the far east forests, growing under conditio...

Holt, A.F., 1952: Analysis of conception rates and other data in artificial insemination. Statistical analysis is made of the results obtained in five years' work at a Cattle Breeding Centre. A conception rate calculated at three weeks can be translated into that for six weeks, nine weeks, three months and the true calving concept...

Tameda, Yukihiko; Ooi, Kinue; Kosaka, Yoshitane; Takase, Kohjiro; Itoh, Nobuyasu, 1993: Enzymatic assay of the ratio of serum branched chain amino acid to tyrosine and its clinical significance in patients with various liver diseases. Mie Medical Journal 43(3): 243-251

Idogawa, H.; Imamura, A.; Onda, M.; Umemura, T.; Ohashi, M., 1998: Progression of articular destruction and the production of tumour necrosis factor-alpha in antigen-induced arthritis in rabbits. We examined the progression of articular destruction and the production of tumour necrosis factor-alpha (TNF-alpha) in antigen-induced arthritis (AIA) in rabbits, i.e. flare-ups of inflammation induced by repeated intra-articular injections (singl...

Lloyd, K.L.uise; Kubes, P., 2003: LPS stimulated endothelium enables leukocyte recruitment via membrane associated CD14. An isotype of CD14 has recently been identified on endothelium but details of this LPS receptor are as yet unknown. First passage endothelial cells were found to be CD14 positive by ELISA. Flow cytometric analysis revealed that the density of CD14...

Danziger, J.; Bloch, S., 1975: The value of the external carotid circulation in the assessment of intracranial disease. The external carotid circulation often contributes a vascular supply in intracranial disease. Adequate radiological evaluation of this circulation is important and may alter the surgical management in these patients.

Atkinson, D.E.; Robinson, N.R.; Sibley, C.P., 1991: Development of passive permeability characteristics of rat placenta during the last third of gestation. To investigate how the passive permeability of the rat placenta changes during the last third of gestation we measured the unidirectional maternofetal clearance (Kmf) of three polar nonelectrolytes. We found that Kmf (.mu.l .cntdot. min-1 .cntdot....

Seigneuric, J.B.; Delaporte, T.; Andreani, J.F.; Cantaloube, D., 1999: Facial trauma in sports: Tentative epidemiological study and its limitations. Our aim was to conduct an epidemiological study in order to better assess the frequency of maxillo-facial sport injuries. Only those activities that are practised within a sport or game association are included in the study. A standard letter was...

Datta, D.V.; Narany, A.P.; Singh, H.; Sukavani, N.; Sharma, S.; Chhuttani, P.N., 1978: Presence of deaminase in the upper gastrointestinal tract (a preliminary report). Journal of the Association of Physicians of India 26(11): 1007-1010