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Chromatin packaging as an indicator of human sperm dysfunction

, : Chromatin packaging as an indicator of human sperm dysfunction. Journal of Assisted Reproduction and Genetics 17(9): 508-514

Understanding the causes of fertilization failure is an important research field in assisted reproductive programs. The present study aimed to evaluated the possible relationship between chromatin packaging quality (CMA3 staining) and (i) normal morphology and (ii) its ability to predict the functional integrity of spermatozoa in both in vitro fertilization (IVF) and intracytoplasmic sperm injection (ICSI) treatment programs. Semen of 140 men from IVF and ICSI couples were analyzed for sperm concentration, motility, morphology, and chromatin packaging (CMA3). For CMA3 classification, two cutoff values were used, namely, 44.5% +/- 13 and 1 SD above the mean, i.e., 57.5% (rounded off to 60%). IVF and ICSI data were stratified using three basic cutoff values for CMA3 staining, namely, < 44%, > 44-60%, and > 60%. Based on CMA3 results patients were divided into four groups, namely, group A, < 44% CMA3 (n = 15, IVF); group B, > or = 44% and < 60% CMA3 (n = 39, IVF); group C, > or = 60% CMA3 (n = 45 IVF); and group D, > or = 60% CMA3 (n = 41 ICSI). During receiver operator characteristic analyses the estimated cutoff value for CMA3 staining, to distinguish between < 4% and > or = 4% morphology groups, was 60%. The area under the curve was 0.89, sensitivity of 75%, and specificity of 100%. When IVF rates of > 60% and < 60% were used, the optimal CMA3 value for prediction of fertilization success again was recorded at 60%. The area under the curve was 0.76, sensitivity of 81.5%, and specificity of 63.6%. Chromatin packaging assessments should be included as a complementary assay to the sequential diagnostic approach of the male-factor patients.


PMID: 11155324

Other references

Krall, M., 1961: The strawberry eelworrn (Apheienchoides fragariae) in Estonia. Faunistilisi Markmeid Tartu, 1: 95-97

Sichel, D.; Wild, C.; Heimendinger, E.; Klotz, A.; Chatelain, A., 1955: Importance of temporal tomography in chronic otorrhea. Journal de Radiologie, D'electrologie & Archives D'electricite Medicale 36(5-6): 461-464

Kirsanov, A.T., 1940: Liming. II. The role of aluminium, manganese and iron in the fertility of podzolized soils and its evaluation in liming.. In a series of pot cultures it is shown that acidity alone is not the cause of reduced yields. The circulation of Fe has a great and sometimes a greater negative effect than Al. The effect of Mn is similar to that of Al and Fe. At low pH values th...

Shilin B.V.; Gusev N.A., 1969: Infrared survey of the Kamchatka volcanoes. Sovetskaya Geologiya 5: 139-147

Naro-Maciel, E.; Gaughran, S.J.; Putman, N.F.; Amato, G.; Arengo, F.; Dutton, P.H.; McFadden, K.W.; Vintinner, E.C.; Sterling, E.J., 2014: Predicting connectivity of green turtles at Palmyra Atoll, central Pacific: a focus on mtDNA and dispersal modelling. Population connectivity and spatial distribution are fundamentally related to ecology, evolution and behaviour. Here, we combined powerful genetic analysis with simulations of particle dispersal in a high-resolution ocean circulation model to inve...

Singh, P.; Pearlman, S., 2017: Use of Computer Imaging in Rhinoplasty: A Survey of the Practices of Facial Plastic Surgeons. The objective of this study was to quantify the use of computer imaging by facial plastic surgeons. AAFPRS Facial plastic surgeons were surveyed about their use of computer imaging during rhinoplasty consultations. The survey collected information...

North, R.B.; Ewend, M.G.; Lawton, M.T.; Piantadosi, S., 1991: Spinal cord stimulation for chronic, intractable pain: superiority of "multi-channel" devices. Spinal cord stimulation has evolved over the past 20 years into an easily implemented technique, with low morbidity, for the treatment of intractable, chronic pain in properly selected patients. We report our experience with a series of 62 patient...

Anonymous, 1981: Asian rickets in Britain. Lancet 2(8243): 402-402

Katz, J.N.; Smith, S.R.; Yang, H.Y.; Martin, S.D.; Wright, J.; Donnell-Fink, L.A.; Losina, E., 2016: The Value of History, Physical Examination, and Radiographic Findings in the Diagnosis of Symptomatic Meniscal Tear among Middle-Age Subjects with Knee Pain. To evaluate the utility of clinical history, radiographic and physical exam findings in the diagnosis of symptomatic meniscal tear (SMT) in patients over age 45, in whom concomitant osteoarthritis is prevalent. In a cross-sectional study of patien...

Berg Otto E., 1971: Five years of data from the Pioneer cosmic dust experiment. Eos, Transactions, American Geophysical Union 52(4): 261