geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Cloning and Sequence Analysis of the Chitinase Gene of Spodoptera litura Nuclear Polyhedrosis Virus


, : Cloning and Sequence Analysis of the Chitinase Gene of Spodoptera litura Nuclear Polyhedrosis Virus. Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao Acta Biochimica et Biophysica Sinica 32(5): 537-540

Using AcMNPV chiA-containing fragment as a probe, the chitinase gene of Spodoptera litura nuclear polyhedrosis virus (SpltNPV) was localized in two contiguous fragments, XbaI 5.1 kb and 2.1 kb, and the intact SpltNPV chiA gene was obtained by cloning and sequencing those two fragments. The complete open reading frame of the gene was 1 695 nucleotide long, encoding a putative protein of 564 amino acids with molecular weight of 62.9 kD. Sequence analysis further revealed that the 5' noncoding region had a baculovirus late promoter motif TAAG. A polyadenylation signal, AATAAA, was located dowmnstream of the translation stop codon. A putative signal peptide was present at the N-terminus of the protein. This gene shares 57% homology with AcMNPV chitinase gene.

US$29.90

PMID: 12058207


Other references

Avery, G.B.; Guandolo, V.L., 1966: Grand rounds: vertebral column (status dysraphicus). Clinical Proceedings - Children's Hospital of the District of Columbia 22(2): 51-56

Engstrøm, T.; Bratholm, P.; Vilhardt, H.; Christensen, N.J., 1997: Effect of pregnancy on rat myometrial beta 2-adrenoceptor mRNA and isoproterenol-induced relaxation of isolated uterine strips. The altered myometrial contractile activity near term of pregnancy is partly due to changes in the responsiveness to catecholamines. Previous experiments have basically been concerned with uterine adrenoceptor binding characteristics. In the prese...

Martin, P.; Rademacher, B., 1960: Studies on the mutual influences of weeds and crops. Four litre culture pots were connected by glass pipes and 2 plant species put in alteruate pots. Richter's nutrient solution was supplied and circulated through the pots. Scopoletin liberated from the root cells of oats had an inhibiting effe...

Mutiso, J.Muli.; Macharia, J.Chege.; Taracha, E.; Gicheru, M.Muita., 2012: Leishmania donovani whole cell antigen delivered with adjuvants protects against visceral leishmaniasis in vervet monkeys (Chlorocebus aethiops). In a previous immunogenicity and efficacy study in mice, montanide ISA 720 (MISA) was indicated to be a better adjuvant than bacillus calmette guerin vaccine (BCG) for a Leishmania vaccine. In the present study, we report the safety, immunogenicit...

Stein, J.H.; Uretz, E.F.; Parrillo, J.E.; Barron, J.T., 1996: Cost and appropriateness of radionuclide exercise stress testing by cardiologists and non-cardiologists. The hypothesis that a diagnostic evaluation performed by a generalist is less expensive than that performed by a specialist is untested. We retrospectively evaluated the indications and financial ramifications of radionuclide exercise stress testi...

Kostia, D., 1996: The Aemodogryllinae (Orthoptera: Rhaphidophoridae) found in the Korean Peninsula. Five species of Aemodogryllinae identified in the Korean Peninsula are described and their distribution within the region is recorded. Illustrations of the male genitalia of all the species are given.

Collins, M.G.; Juvina, I.; Gluck, K.A., 2016: Cognitive Model of Trust Dynamics Predicts Human Behavior within and between Two Games of Strategic Interaction with Computerized Confederate Agents. When playing games of strategic interaction, such as iterated Prisoner's Dilemma and iterated Chicken Game, people exhibit specific within-game learning (e.g., learning a game's optimal outcome) as well as transfer of learning between ga...

Ekmekci, H.; Kaptan, H., 2016: Camptocormia and deep brain stimulation: The interesting overlapping etiologies and the therapeutic role of subthalamic nucleus-deep brain stimulation in Parkinson disease with camptocormia. Camptocormia is known as "bent spine syndrome" and defined as a forward hyperflexion. The most common etiologic factor is related with the movement disorders, mainly in Parkinson's disease (PD). We present the case of a 51-year-old...

Wang, J.; Tian, Y.; Shao, Y.; Feng, H.; Qin, L.; Xu, W.; Liu, H.; Xu, Q.; Wei, S.; Ma, L., 2017: Comparison of spontaneous brain activity revealed by regional homogeneity in AQP4-IgG neuromyelitis optica-optic neuritis versus MOG-IgG optic neuritis patients: a resting-state functional MRI study. Many previous studies have demonstrated that neuromyelitis optica (NMO) patients have abnormalities of brain anatomy and function. However, differences in spontaneous brain activity between myelin oligodendrocyte glycoprotein (MOG)-IgG ON and aqua...

Victorov G.A.; Kochetova N.I., 1973: On the regulation of the sex ratio in dahlbominus fuscipennis hymenoptera eulophidae. Entomological Review (English Translation of Entomologicheskoye Obozreniye) 52(3): 434-438