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Culture with Matrigel inhibits development of mouse zygotes


, : Culture with Matrigel inhibits development of mouse zygotes. Journal of Assisted Reproduction and Genetics 14(9): 543-548

It was reported that Matrigel improved hatching of mouse blastocysts produced in vitro from F1 hybrid-derived zygotes. We investigated whether Matrigel would be similarly beneficial with outbred strain-derived embryos, which exhibit a "two-cell" block similar to the developmental blocks of other species. Mouse embryo development was assessed with or without Matrigel in KSOM medium, which supports the development of blocking strain zygotes in vitro, and in human tubal fluid (HTF) medium, which normally does not but which is used for human IVF. Matrigel severely inhibited the development of zygotes to blastocysts in KSOM and did not improve culture in HTF. There was no effect on development from the two-cell stage. We were not able to replicate the previous finding of Matrigel's beneficial effect on hatching of F1-derived zygotes. Matrigel may be a deleterious addition to embryo culture or coculture systems.

US$29.90

PMID: 9401874


Other references

Strazhnikova L.V., 1975: Calculation of the linear growth of bream from the mingechaur ussr reservoir by the method of empirical scales. Izvestiya Akademii Nauk Azerbaidzhanskoi SSR Seriya Biologicheskikh Nauk (3): 99-103

Mccuaig, L., 1976: Interference by divalent metals in the preparation of soluble intestinal alkaline phosphatase with n butanol. Certain factors prevented quantitative recovery of soluble alkaline phosphatase from homogenates of chick duodenal mucosa during treatment with n-butanol. Divalent cations such as Ca2+, Mn2+ and Pb2+ interfered when present at 0.1-0.2 mM. Mg2+ and...

Colyvas N.; Phillips N.R.; Stoney R.J.; Havel R.J.; Rapp J.H., 1989: Lipoprotein profiles of patients with recurrent carotid stenosis. Surgical Forum (Chicago) 40: 281-283

Coates, A.; Schaefer, O.; Uy, K.; O'Sullivan, B.P., 2013: Empyema in a woman with cystic fibrosis: a cautionary tale. Cystic fibrosis (CF) is a disease which predisposes individuals to recurrent infective exacerbations of suppurative lung disease; however, empyema is a rare complication in these patients. Empyemas secondary to Staphylococcus aureus and Burkholder...

Bellingham, R.A., 1982: Hospital wellness programs aid employees. Employee Health & Fitness 4(3): 25-27

Sutor, A.H., 1999: Desmopressin (DDAVP) in bleeding disorders of childhood. As in adults, desmopressin (DDAVP) can be used in children for prophylaxis of bleeding and to stop bleeding in many hereditary and acquired bleeding disorders. DDAVP is the treatment of choice in children with mild hemophilia and type 1 von Willeb...

Cerda Reverter, J.M.guel; Zanuy, S.; Carrillo, M.; Kah, O., 1996: Development of enzyme immunoassays for 3,5,3-Triiodo-L-thyronine and L-thyroxine Time-course studies on the effect of food deprivation on plasma thyroid hormones in two marine teleosts, sea bass and sea bream. The effects of short-term food deprivation and photoperiod on plasma thyroid hormone levels of sea bass and sea bream were studied. Animals were acclimated under constant photoperiod regime (15L/9D) and feeding times (2 hr after light onset and 2...

Dunlap, S.; Heinrichs, S.C., 2008: Neuronal depletion of omega-3 fatty acids induces flax seed dietary self-selection in the rat. The impact of essential dietary fatty acid deficiency on self-selection of fatty acid enriched foods is little studied in spite of widespread health promotion claims for fatty acid supplemented diets. Accordingly, the present studies investigated...

Bol, S.J.; Tucker, D.P.; Ekert, H., 1990: Selection of two human bone marrow stromal cell subpopulations with different effects on the maintenance and differentiation of myeloid progenitor cells. The simple selection of two human bone marrow stromal cell populations is described. Adherent stromal cell layers were formed in primary cultures of low-density marrow cells. At time of confluence, persistent nonadherent cells were collected and t...

Rodriguez, N.; Lim, S.; Pannell, L.; Morasso, M., 2003: Identification of DLx3 interacting factors using a retroviral system and mass spectrometry analysis. Journal of Investigative Dermatology 121(1): 0656, July