+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Curative effect of picroliv on primary cultured rat hepatocytes against different hepatotoxins: an in vitro study

, : Curative effect of picroliv on primary cultured rat hepatocytes against different hepatotoxins: an in vitro study. Journal of Pharmacological and Toxicological Methods 40(3): 173-179

Picroliv, the standardized active principle from the plant Picrorhiza kurrooa showed significant curative activity in vitro in primary cultured rat hepatocytes against toxicity induced by thioacetamide (200 microg/mL), galactosamine (400 microg/mL), and carbon tetrachloride (3 microl/mL). Activity was assessed by determining the change in hepatocyte viability and rate of oxygen uptake and other biochemical parameters (GOT, GPT, and AP). The toxic agents alone produced a 40-62% inhibition of cell viability and a reduction of biochemical parameters after 24 h of incubation at 37 degrees C which (on removal of the toxic agents) was reversed after further incubation for 48 h. Incubation of damaged hepatocytes with picroliv exhibited a concentration- (1-100 microg/mL) dependent curative effect in restoring altered viability parameters. The results warrant the use of this in vitro system as an alternative for in vivo assessment of hepatoprotective activity of new agents.


PMID: 10334634

Other references

Solov'ev, A.E., 1986: Remote results of the surgical treatment of urinary calculi in children. An analysis of 144 case histories of children operated upon for calculi in urinary ducts has shown that in most cases technical errors and complications resulting from surgery were responsible for recidivation of urolithiasis in children.

Vaartaja, O.; King, J., 1964: Fungi associated with a wood wasp in dying pines IPinus radiatal in Tasmania. Phytopathology 54(8): 1031-1032

Kojan C.J.; Sanders A.J.; Morris P.A.; Bradley J.J.; Faulkner J.A., 1996: The use of regional-scale regolith chemistry in evaluating Ni and Au mineralisation in granite-greenstones of the northern Eastern Goldfields, Western Australia. Pages 41 1996

Frederick, L.D.; Robinson, H.E., 1941: Canine nutritional deficiency diseases. The functions in canine metabolism of the known vitamins, their sources, and descriptions of their deficiency symptoms in dogs are reviewed.

Rizun B.P.; Chizh J.I., 1986: Peculiarities of the arrangement and systematization of fractures in the Volyn-Podolia Plate. Dopovidi Akademiyi Nauk Ukrayins'koyi RSR, Seriya B: Geologichni, Khimichni ta Biologichni Nauki 1986(11): 22-25

Morris, C.; Siegel, E.; Cottler Fox, M.; Tricot, G.; Badros, A.; Desikan, R.; Anaissie, E.; Toor, A.; Zangari, M.; Barlogie, B., 2000: Stem cell mobilization in patients gtoreq 70 years with multiple myeloma Influence of prior therapy and mobilizing regimen. Little is known about the ability to mobilize Peripheral Blood CD34+ Stem Cells (PBSCs) in MM patients gtoreq 70 years old, although the median age of MM pts is 67 yrs and elderly pts benefit equally from high-dose therapy (HDT) followed by autotr...

Daughaday, W.H.; Cryer, P.E., 1978: Growth hormone hypersecretion and acromegaly. Hospital Practice 13(8): 75-80

Ben Tovim D.I., 1978: The psychiatric muenchausen. Postgraduate Medical Journal 54(638): 818-820

Oniani O.G.; Margvelashvili G.N.; Dzhavakhishvili G.A., 1990: Effectiveness of compound and concentrated phosphorus fertilizers on chernozem and gray brown soils of eastern georgia ussr. Agrokhimiya (11): 93-102

Ho, I Cheng; Glimcher, L.H., 2002: Transcription: tantalizing times for T cells. The T helper lymphocyte is responsible for orchestrating an appropriate immune response to pathogens. To do so, it has evolved into two specialized subsets that direct type 1 and type 2 immunity. Here, we discuss the genetic programs that control...