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Curcumin in plasma and urine: quantitation by high-performance liquid chromatography


, : Curcumin in plasma and urine: quantitation by high-performance liquid chromatography. Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences 783(1): 287-295

Curcumin, a derivative of the plant Curcuma longa, is used extensively in the food industry. It is a major component of curry powder, and research has shown that curcumin may prevent cancer and other chronic diseases. We have developed a robust automated analytical method for the determination of curcumin in plasma and urine. The method involves extracting the curcumin from 0.2 ml sample volume with ethyl acetate/methanol organic solvents, and use of an internal standard, beta-17-estradiol acetate. Analysis utilizes a reversed-phase C(18) column and UV detection at 262 nm. Performance characteristics have been assessed. The assay is linear from 0.2 to 7.0 microgram/ml. The coefficient of variation for intra- and inter-day assays is <7.5%. The average recovery of curcumin from plasma and urine is greater than 96%. The data presented in this report demonstrate that the method provides rapid, sensitive, precise and accurate measurements of curcumin concentrations in plasma and urine.

US$29.90

PMID: 12450549


Other references

Kosova, J., 1977: Development of large scale fruit production in Bulgaria. Zahradnictvo 2 (10) 450

Bay, A.; Coskun, E.; Oztuzcu, S.; Ergun, S.; Yilmaz, F.; Aktekin, E., 2013: Evaluation of the plasma micro RNA expression levels in secondary hemophagocytic lymphohistiocytosis. Hemophagocytic lymphohistiocytosis (HLH) is a life threatening hyper inflammatory disease. Micro RNAs (miRNA) are about 22 nucleotide-long, small RNAs encoded with genes, and they have regulatory functions in immune response. To determine the miRN...

von Hippel, P.H., 1994: Protein-DNA recognition: new perspectives and underlying themes. Science 263(5148): 769-770

Brocher, Frank., 1914: Physiologie de la respiration chez les Insectes imagos. Archives de Zoologie Paris, 54

Chinenye, S., 2002: The current classification and diagnostic criteria for diabetes mellitus. Nigerian Journal of Medicine 11(2): 84-86

Ueda, N.; Nagakawa, T.; Ohta, T.; Kayahara, M.; Ueno, K.; Konishi, I.; Izumi, R.; Miyazaki, I., 1992: Synchronous cancer of the biliary tract and pancreas associated with anomalous arrangement of the pancreaticobiliary ductal system. A 58-year-old man on abdominal ultrasonography and CT had an irregularly elevated lesion at the neck of the gallbladder and a cyst of approximately 6.5 cm in diameter at the pancreatic tail. Percutaneous transhepatic cholangiography revealed a 2-c...

Wardekar, A.S.; Son, B.S.; Bercu, B.A., 1973: Myocardial infarction and oral contraceptives. Southern Medical Journal 66(3): 382-384

Franchi A.M.; Chaud M.; Gonzalez E.T.; Gimeno M.A.F.; Gimeno A.L., 1988: Effects of experimental diabetes on spontaneous contractions on the output of prostaglandins and on the metabolism of labelled arachidonic acid in uteri isolated from ovariectomized rats influences of estradiol. Spontaneous changes in isometric developed tension (IDT) as a function of time after isolation (contractile constancy) in uteri from control-castrated and castrated chronic streptozotocin-diabetic rats, were explored. The effects of injecting 17-b...

Coles, M.R., 1963: Southern Children Under Desegregation. American Journal of Psychiatry 120: 332-344

Heintz, L.L., 1988: Hawaii's nutrition and hydration fix: will it be medico-moral, judicial or legislative?. Hawaii Medical Journal 47(12): 560, 563-70