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Differentiation of isomeric photomodified oligodeoxynucleotides by fragmentation of ions produced by matrix-assisted laser desorption ionization and electrospray ionization


, : Differentiation of isomeric photomodified oligodeoxynucleotides by fragmentation of ions produced by matrix-assisted laser desorption ionization and electrospray ionization. Journal of the American Society for Mass Spectrometry 10(4): 329-338

UV irradiation of oligodeoxynucleotides at 254 nm generates several different types of DNA photoproducts, including cis-syn cyclobutane pyrimidine dimers, pyrimidine[6-4] pyrimidone photoproducts and their Dewar valence isomers, and thymine-adenine photoproducts (TA*). Studies of photoproducts in oligodeoxynucleotides require the development of suitable structure determination methods such as mass spectrometry. In an earlier study (Vollmer et al. Int. J. Mass Spectrom. Ion Processes 1997, 165/166, 487-496), we showed that fast atom bombardment and tandem sector mass spectrometry can be used to locate the site of photomodification and identify most of the photoproducts of d(TATTAT). One goal of the present research was to expand the method to the more sensitive electrospray ionization (ESI) and matrix-assisted laser desorption/ionization (MALDI) methods. A second goal was to test the generality of the methods by investigating not only the photoproducts of d(TATTAT) but also those of three other oligodeoxynucleotides, d(GTATTAT), d(GGCTATAA), and d(AATTAA). The photoproducts of these sequences were separated by HPLC and gave characteristic fragment ions in postsource decompositions of MALDI-produced ions and collisionally activated decompositions of ESI-produced ions.

US$19.90

PMID: 10197353

DOI: 10.1016/S1044-0305(98)00158-5


Other references

Lee, D.Y.; Hong, S.K.; Lee, P.H., 1965: Physical Insulation Of Healthy Men And Women Over 60 Years. Maximal body insulation [I = (rectal temp. - skin temp.)/rate of skin heat loss] of healthy Korean men and women (17 each) over 60 years of age was determined by a method applied earlier to diving women of Korea (Rennie et al. J. Appl. Physiol. 17...

Fievet, P.; Desablens, B.; Joly, J.P.; Fournier, A., 1984: Anuria caused by a neoplastic obstacle, reversible under chemotherapy. Presse Medicale 13(24): 1512-1513

Cline, M.J.; R.Malcolm, 1967: Synthesis of macro-globulins in vitro fhuman neqplastic cell lymphosarcoma chronic lymphocytic rabbit leukemia. Nature 214(5087): 496-497

Frebet, E.; Preckel, M.P.; Poirier, N.; Mercier, P.; Granry, J.C., 2000: Evaluation of transfusion strategies during craniosynostosis repairs. To evaluate the changes in transfusion practices during craniosynostosis surgery in children, with consideration of the transfusion-associated risks. Retrospective study. The study included 64 consecutive craniosynostosis repairs by the same neuro...

Li, Y.; Zhang, R.; Li, D., 2016: A Systematic Review of Levels of Evidence in Auricular Defects Literature: How Far Has It Been During the Past Three Decades?. Evidence-based medicine has been increasingly applied in clinical practice. The objective of this study was to identify the trends of levels of evidence and the relevant factors of higher level evidence in external auricular defects literature ove...

Lawson, JR., 1990: Transmission of taeniid tapeworms eggs via blowflies to intermediate hosts. Experiments showed that dead blowflies containing eggs of Taenia hydatigena can transmit infection if ingested by lambs during grazing. Rabbits became infected with T. pisiformis after exposed to blowflies that been in contact with dog faeces cont...

Lamarche, M.; Tapin, M., 1961: Action of a mixture of the salts of aspartic acid on the isolated heart of the guinea pig. Comptes Rendus des Seances de la Societe de Biologie et de Ses Filiales 155: 377-379

Cook, G.C., 1989: Communicable and tropical diseases. This book, composed of 18 chapters, covers those diseases which are capable of being transmitted from one organism to another. The topics covered include: childhood infections; tuberculosis; malaria; infections of the gastrointestinal tract, liver...

Lepage, G., 1980: Biology of keratinized squamous epithelium. Acta Oto-Rhino-Laryngologica Belgica 34(4): I-Xv

Lähteenmäki, M.; Sormunen, E.; Koivisto, M.; Railo, H.; Tuominen, L., 2015: TMS-Induced Seizure Following Focal Single-Pulse IPS Stimulation. Brain Stimulation 8(6): 1238-1238