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Distinct kinetic properties of cardiac myosin isoforms revealed by in vitro studies


, : Distinct kinetic properties of cardiac myosin isoforms revealed by in vitro studies. Advances in Experimental Medicine and Biology 453: 125-130

To clarify the physiological significance of myosin isoform redistribution in cardiac adaptation process, we compared the kinetic property of the two cardiac myosin isoforms using in vitro motility assay techniques. Cardiac myosin isoforms V1 and V3 were obtained from ventricular muscle of young rats and hypothyroid rats respectively. On each of these myosin isoforms fixed on a glass coverslip, fluorescently labeled actin filaments were made to slide in the presence of ATP. To measure the force generated by actomyosin interaction, a small latex bead was attached to the barbed end of an actin filament and the bead was captured by the laser optical trap installed in a microscope. The force was determined from the distance between the bead and the trap positions under either auxotonic or isometric conditions. The time-averaged force generated by multiple cross-bridges did not differ significantly between the two isoforms. On the other hand, the unitary force measurement revealed the same level of amplitude but a longer duration for V3 isoform. The same level of time-averaged force is in agreement with not only our previous finding but the results of maximum force measurement in muscle preparations. The difference in kinetic characteristics of the two isoforms could account for the difference in economy of force development and the basis for cardiac adaptation mechanism.

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PMID: 9889822


Other references

Marinelli; Baroni; Meloni, 1988: Structural and electronic properties of spinel semiconductors: An ab initio pseudopotential study of MgIn2S. Physical Review. B, Condensed Matter 38(12): 8258-8263

Sertoli, P., 1951: Relation between vitamin F 99 and cholesterol metabolism; preliminary note. Archivio Per le Scienze Mediche 92(4): 240-253

Kaiser, Jocelyn, 1996: Asking for the money up front. The White House is proposing to provide a number of projects with lump sums to ensure that they are completed. Some agencies engaged in particular projects must request funding from Congress each year so that work can continue. This approach can r...

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Mauersberger, W.; Diaz, L.A., 1985: Sphenopharyngeal encephalocele associated with agenesis of the corpus callosum. A case of a 38-year-old patient, who had been complaining for some years about impairment in nasal breathing, is presented. Within the last few months the patient presented a rapid deterioration of his visual acuity. Radiological examinations reve...

Honjo I.; Ushiro K.; Mitoma T.; Tashima K., 1984: Eustachian tube function of children with secretory otitis media. The Eustachian tube function of children with secretory otitis media was examined by several methods: endoscopy of the pharyngeal orifice of the tube; air inflation of the tube by catheter; tympanometry, inflation and deflation test for non-intact...

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Palit S.; Sen S.; Sharma A.; Talukder G., 1991: Protection by chlorophyllin against induction of chromosomal aberrations by cobalt in bone marrow cells of mice in vivo. Interaction between cobaltous chloride and chlorophyllin [a food additive] was studied, using as endpoint the frequency of chromosomal aberrations and breaks per cell induced in bone marrow cell of male mice. Two concentrations of CoCl2 (20 nad 40...

Rafuse, J., 1997: Negative health care environment weighing on residents, survey reveals. Cmaj 156(8): 1186-1186