+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Effect of Angelica sinensis injection on CD11c and CD14 expression in alveolar macrophage membrane of chronic bronchitis patients

, : Effect of Angelica sinensis injection on CD11c and CD14 expression in alveolar macrophage membrane of chronic bronchitis patients. Zhongguo Zhong Xi Yi Jie He Za Zhi Zhongguo Zhongxiyi Jiehe Zazhi 19(5): 282-285

To explore the effect of Angelica sinensis injection (ASI) in CD11c and CD14 expression in alveolar macrophage (AM) membrane of chronic bronchitis patients. AM from 10 chronic bronchitis patients (remmision stage) and 10 healthy subjects was obtained by bronchoalveolar lavage. After had cultured for 24 hours with ASI or/and lipopolysaccharide (LPS), CD11c and CD14 expression and intracellular calcium ion concentration in AM were analyzed by flow cytometry. CD11c and CD14 expression levels of patients' AM membrane (n = 10) were (39.17 +/- 5.56)% and (35.73 +/- 8.05)% respectively, which were significantly higher than those of the control [n = 10, (16.29 +/- 3.78)% and (15.26 +/- 5.96)%], P < 0.05. LPS could increase the elevation of CD11c expression of patients [(47.25 +/- 7.00)%, P < 0.05], while ASI could reduce the increment [LPS Plus ASI group (27.61 +/- 8.64)%, P < 0.05]. The basic calcium level of AM cytoplasma of patients was (189.47 +/- 23.69) nmol/L (n = 7), which was higher than that of healthy control (99.65 +/- 32.21) nmol/L (n = 6), P < 0.05. The intracellular calcium ion elevation in AM of patients could be induced by LPS, and ASI could inhibit the elevation. The calcium level in LPS group was (288.47 +/- 43.68) nmol/L, in ASI Plus LPS group, (236.68 +/- 28.60) nmol/L, P < 0.05. ASI could reduce the CD11c expression in AM of chronic bronchitis patients by inhibiting LPS induced intracellular calcium ion elevation in AM, suggesting that ASI may inhibit non-specific inflammation of respiratory tract.


PMID: 11783242

Other references

Bogg, J.; Gibbs, T.; Bundred, P., 2001: Training, job demands and mental health of pre-registration house officers. This study aims to explore pre-registration house officer (PRHO) perceptions of work role, job requirements and mental health, in order to enhance work role preparation by means of identifying potential areas for curriculum development. Phase 1 to...

Haydock, A.K.; Porat, I.; Whitman, W.B.; Leigh, J.A., 2004: Continuous culture of Methanococcus maripaludis under defined nutrient conditions. To study global regulation in the methanogenic archaeon Methanococcus maripaludis, we devised a system for steady-state growth in chemostats. New Brunswick Bioflo 110 bioreactors were equipped with controlled delivery of hydrogen, nitrogen, carbon...

Waldman, R.K.; Ullrich, L.F.; Borman, E.K., 1950: Adaptation of the VDRL method for total protein determination to the use of small quantities of cerebrospinal fluid. A modification of the VDRL method for total protein content of cerebrospinal fluid specimens is described in detail. It makes use of only 0.5 ml. of specimen and of optically matched tubes of standard size with the Coleman spectrophotometer, model...

Anonymous, 1936: Utah State Agricultural College, Agricultural Experiment Station. Biennial Report of the Director, 1934-35 and 1935-36. 162. Phosphorus supplements in sugar beet by-product rations (11). 163. Phosphorus deficiency in steers (12). 164. Skimmilk, buttermilk, and whey for pigs (13). 165. Availability of phosphorus from different compounds (14). 166. Production of wool...

Veith, F.J., 2010: The management of ruptured abdominal aortic aneurysms. Journal of Cardiovascular Surgery 50(5): 573-574

Zhaohui, Y.; Xiaolin, J.; Xiancai, R.; Xiaoxing, C.; Fuquan, H., 2007: A novel strategy for systematic identification of natural antisense transcripts of Pseudomonas aeruginosa based on RNase I protection assay. Natural antisense transcripts (NATs) are widespread in prokaryotes and eukaryotes. They have very important functions in regulating expression of their target genes. However, until now, NATs haven't been systematic identified in high throughp...

Hutchings, J.J., 1960: Metabolic diseases of childhood. Journal of Chronic Diseases 12: 391-396

Siegler, I.; Viaud Delmon, I.; Israel, I.; Berthoz, A., 1998: Perception of two consecutive whole-body rotations and their concurrent eye movements. Society for Neuroscience Abstracts 24(1-2): 415

Dobler H J., 1976: Development of thermo regulation in the new born lesser bush baby galago senegalensis moholi. Experientia (Basel) 32(2): 194-195

Cornwell, J.R.; Bartek, J.K., 1996: Phenazopyridine hydrochloride: information for patient teaching. Urologic Nursing 16(4): 153-154