geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Endothelial gene regulation by laminar shear stress


, : Endothelial gene regulation by laminar shear stress. Advances in Experimental Medicine and Biology 430: 155-164

Endothelial cells, because of their unique localization, are constantly exposed to fluid mechanical forces derived by the flowing blood. These forces, and more specifically shear stresses; affect endothelial structure and function, both in vivo and in vitro, and are implicated as contributing factors in the development of cardiovascular diseases. We have demonstrated earlier that the shear stress selectively induces the transcription of several endothelial genes, and have defined a shear stress response element (SSRE) in the promoter of platelet-derived-growth-factor B (PDGF-B), that is shared by additional endothelial shear stress responsive genes. Here we further characterize this SSRE and the nuclear factors that bind to it, and imply the possible role of the endothelium cytoskeleton in transducing shear stress, leading to the expression of PDGF-B/SSRE constructs in transfected endothelial cells exposed to shear stress. We also present, yet a new shear stress response element in the Platelet Derived Growth Factor A promoter, that contains a binding site to the transcription factors egr1/sp1. These results further demonstrate the complexity of gene regulation by hemodynamic forces, and support the important part that these forces have in the physiology and pathophysiology of the vessel wall.

US$29.90

PMID: 9330726


Other references

Anderson, G.P.; Sanderson, D.G.; Lee, C.H.; Durell, S.; Anderson, L.B.; Gross, E.L., 1987: The effect of ethylenediamine chemical modification of plastocyanin on the rate of cytochrome f oxidation and P-700+ reduction. Chemical modification of plastocyanin was carried out using ethylenediamine plus a water-soluble carbodiimide, which has the effect of replacing a negatively charged carboxylate group with a positively charged amino group at pH 6-8. The conditions...

Sezer, E.; Ozugurlu, F.; Ozyurt, H.; Sahin, S.; Etikan, I., 2007: Lipid peroxidation and antioxidant status in lichen planus. Lichen planus (LP) is an inflammatory skin disease of unknown aetiology. Recently, increased oxidative stress has been implicated in the pathogenesis of various skin diseases such as atopic dermatitis, psoriasis vulgaris and vitiligo. To evaluate...

Ochi, H.; Hirani, W.M.; Yuan, Q.; Friend, D.S.; Austen, K.F.; Boyce, J.A., 1999: T helper cell type 2 cytokine-mediated comitogenic responses and CCR3 expression during differentiation of human mast cells in vitro. Mast cells (MCs) arise in situ from circulating stem cell factor (SCF)-dependent committed progenitors (PrMCs) and accumulate at sites of allergic mucosal inflammation. We hypothesized that human (h)PrMCs and their mature counterparts might share...

Mcneil, S.; Landwehrmeyer, G.B.; Dure, L.S.; Ge, P.; Aizawa, H.; Huang, Q.; Difiglia, M.; Golden, J.; Bird, E., 1993: Distribution of IT 15 mRNA in human and rat brain. Society for Neuroscience Abstracts 19(1-3): 408

Falcon Rafael; Lagazzi R.; Sampson Elizabeth, 1993: Reference sections for the informal members C-1/ 7, B-6/ 9 and B-1/ 5 of the Misoa Formation. Pages 32 1993

Cech, A.C.; Morris, J.B.; Mullen, J.L.; Crooks, G.W., 1995: Long-term enteral access in aspiration-prone patients. Aspiration pneumonia is a serious complication of enteral feeding. Many critically ill patients are particularly at risk for aspiration. Few studies have rigorously compared various access devices. Risk factors for aspiration and studies examining...

Levy J.V.; Blake K., 1986: Inhibition of atp secretion and aggregation of human platelets induced by platelet activating factor. Federation Proceedings 45(3): 226

Beretti A.; Ambroise Daniel, 1980: Evidence of phylogeny of the genus Lepidocyclina Gumbel in Madagascar; measure of the degree of evolution, stratigraphic application. Annales des Mines et de la Geologie 28: 61-83

Ghulghazaryan, R.G.; Sargsyan, K.G.; Ananikian, N.S., 2007: Partition function zeros of the one-dimensional Blume-Capel model in transfer matrix formalism. Zeros of the partition function of the one-dimensional ferromagnetic and antiferromagnetic Blume-Capel models have been studied by using the transfer matrix method in the thermodynamic limit and for finite size chains. The equation for the distrib...

Lin, G.F.; Gebhart, C.J.; Murtaugh, M.P., 1991: Southern blot analysis of strain variation in Campylobacter mucosalis. A panel of three DNA probes were derived at random from a genomic DNA library of Campylobacter mucosalis strain E8384-4. Each probe hybridized specifically to C. mucosalis DNA from bacteria fixed to nylon membranes. The probes did not hybridize to...