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Endothelium-dependent vasorelaxation in inhibited by in vivo depletion of vascular thiol levels: role of endothelial nitric oxide synthase

, : Endothelium-dependent vasorelaxation in inhibited by in vivo depletion of vascular thiol levels: role of endothelial nitric oxide synthase. Free Radical Research 35(4): 387-394

Thiols like glutathione may serve as reducing cofactors in the production of nitric oxide (NO) and protect NO from inactivation by radical oxygen species. Depletion of thiol compounds reduces NO-mediated vascular effects in vitro and in vivo. The mechanisms underlying these actions are not clear, but may involve decreased synthesis of NO and/or increased degradation of NO. This study investigates the effect of glutathione depletion on the response to NO-mediated vasodilation induced by acetylcholine (Ach, 10 micrograms/kg), endothelial NO synthase (eNOS) activity and potential markers of vascular superoxide anion (O2.-) production in conscious chronically catheterized rats. Thiol depletion induced by buthionine sulfoximine (BSO, 1 g i.p. within 24 h) decreased the hypotensive effect of Ach by 30% (MAP reduction before BSO 27 +/- 3 mmHg, 19 +/- 3 mmHg after BSO, (mean +/- SEM), p < .05, n = 8). The impaired effect of Ach was associated with a significant reduction in eNOS activity (control: 7.7 +/- 0.8, BSO: 3.9 +/- 0.4 pmol/min/mg protein (p < .05), n = 6). In contrast, neither NADH/NADPH driven membrane-associated oxidases nor lucigenin reductase activity were significantly (p < .05) affected by BSO (BSO: 4415 +/- 123, control: 4105 +/- 455 counts/mg; n = 6) in rat aorta. It is concluded that in vivo thiol depletion results in endothelial dysfunction and a reduced receptor-mediated vascular relaxation. This effect is caused by reduced endothelial NO formation.


PMID: 11697135

Other references

Zhang, B.; Saku, K.; Shirai, K.; Takao, M.; Arakawa, K., 2000: The association of FERHDL with gender, age, body mass index, and coronary artery disease. Journal of the American College of Cardiology 35(2 suppl A): 309A, Feb

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Ruebenbauer, T.S.iss, L., 1976: Self incompatibility in Gramineae. Postepy nauk rolniczych: 3 (5) 49-76

Diem, P.; Teuscher, A., 1979: Immunologic studies with fully synthetic human insulin in patients with diabetes mellitus. A group of 12 diabetic patients with insulin allergy previously documented by positive skin tests have been investigated with insulin of bovine, porcine and human origin and with a synthetic human insulin (CGP 12 831). In 3 patients clearly positi...

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Matveev B.S., 1970: Origin of conodonts based on evolutionary morphology data. Voprosy Ikhtiologii 10(1): 3-14

Mertz, E.; Mouradian, W.E., 2010: Addressing children's oral health in the new millennium: trends in the dental workforce. Oral Health in America: A Report of the Surgeon General (SGROH) and National Call to Action to Promote Oral Health outlined the need to increase the diversity, capacity, and flexibility of the dental workforce to reduce oral health disparities. Th...

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Hartman, O., 1951: Fabricinae (feather-duster polychaetous annelids) in the Pacific. Pacific Science, 54: 379-391

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