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Expression and insecticidal characteristics of cry3A in Lepidoptera-specific Bacillus thuringiensis

, : Expression and insecticidal characteristics of cry3A in Lepidoptera-specific Bacillus thuringiensis. Wei Sheng Wu Xue Bao 40(2): 139-142

The cry3A gene coding Coleoptera-specific protein was transformed into a wild strain YBT803-1 by electroporation, A transformant BMBY-001 was obtained. SDS-PAGE analysis demonstrated that cry3A could express 64 kD protein in BMBY-001. However, the expression of cry1Ab and cry1Ac were effected. Bioassay showed that BMBY-001 was not only highly toxic to Phyllodecta vulgatissima larva(LC50 0.413 microL/mL) but also toxic to Plutella xylostella(LC50 3.319 microL/mL).


PMID: 12548935

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