geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Expression and insecticidal characteristics of cry3A in Lepidoptera-specific Bacillus thuringiensis


, : Expression and insecticidal characteristics of cry3A in Lepidoptera-specific Bacillus thuringiensis. Wei Sheng Wu Xue Bao 40(2): 139-142

The cry3A gene coding Coleoptera-specific protein was transformed into a wild strain YBT803-1 by electroporation, A transformant BMBY-001 was obtained. SDS-PAGE analysis demonstrated that cry3A could express 64 kD protein in BMBY-001. However, the expression of cry1Ab and cry1Ac were effected. Bioassay showed that BMBY-001 was not only highly toxic to Phyllodecta vulgatissima larva(LC50 0.413 microL/mL) but also toxic to Plutella xylostella(LC50 3.319 microL/mL).

US$29.90

PMID: 12548935


Other references

Wang, Z.Z., 1989: Inoculation and main points in management of fungus cultivation on logs. The most important aspects of the cultivation of fungi on logs are discussed briefly. The choice of strains giving high yield and quality is important. A procedure for the inoculation of logs in slots 10-15 cm long and 1.5 cm deep, spaced 6-8 cm a...

Mosher R.S., 1970: Robots that are extensions of man. Impact of Science on Society (English Edition) 20(4): 349-361

Fukuta, S.; Amari, R.; Tsutsui, T., 2016: Double arthroscopic transtendon repair of partial-thickness articular surface tears of the rotator cuff: a surgical technique. This study describes a technique of simultaneous arthroscopy and bursoscopy for transtendon repair of a partial-thickness articular surface rotator cuff tear. All procedures are under simultaneous visualisation from both the glenohumeral joint and...

Thibaut, T.; Meinesz, A.; Amade, P.; Charrier, S.; De-Angelis, K.; Ierardi, S.; Mangialajo, L.; Melnick, J.; Vidal, V., 2001: Elysia subornata (Mollusca) a potential control agent of the alga Caulerpa taxifolia (Chlorophyta) in the Mediterranean Sea. The biological characteristics of Elysia subornata (Mollusca: Opisthobranchia) were studied in an aquarium to assess its risks and chance of success as a potential biological control agent against the invasive alga Caulerpa taxifolia (Chlorophyta)...

Martin, M.; Harding, J., 1981: Evidence for the evolution of competition between two species of annual plants. Evolution 35(5): 975-987

Pikuła, Mł.; Marek-Trzonkowska, N.; Wardowska, A.; Renkielska, A.; Trzonkowski, P., 2014: Adipose tissue-derived stem cells in clinical applications. In the past decade human adipose tissue has been identified as a source of multipotent stem cells. Adipose tissue derived stem cells (ASCs) are characterised by immunosuppressive properties and low immunogenicity. Therefore, they can be used in re...

Neumeier, D.; Knedel, M.; Würzburg, U.; Hennrich, N.; Lang, H., 1975: Determination of creatine phosphokinase-MB in the serum of patients with myocardial infarction by an immunological method. The immunological method of determining creatine phosphokinase-MB in the serum of patients with myocardial infarction described here is based on the differential measurements of CK-activities before and after a specific immuno-precipitation of the...

Maltabar, L.M.; Unguryanu, S.I.; Trakhtman, I.M.; Shimanovich, B.I., 1976: Raising vine transplants hydroponically in greenhouses twice in one year. Vine grafts were planted at 100-400/m2, one batch in late March and another in early May, after the first batch had been removed for planting out. The second batch was removed in late October and stored during the winter for planting out in the fo...

Woodcock, C.L.; Nikitina, T.; Grigoryev, S.A.; Pehrson, J.R., 2000: Dynamic re-distribution of the histone variant macroH2A accompanying large scale chromatin remodeling in lymphocytes. Molecular Biology of the Cell 11(Supplement): 306a, Dec

Brdiczka, D.; Barnard, T., 1980: Mitochondrial matrix granules in soft tissues 2. isolation and initial characterization of a calcium precipitable soluble lipo protein subfraction from brown fat and liver mitochondria. Isolated mitochondria from lamb brown fat and rat liver were subject to hypotonic shock to free the matrix granules. Total mitochondrial membranes were collected by a 2-stage high-speed centrifugation. By addition of Ca ions to the supernatant, me...