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Expression of EGF and its mRNA in human pancreatic carcinoma cell lines and in vivo tumor lines

, : Expression of EGF and its mRNA in human pancreatic carcinoma cell lines and in vivo tumor lines. Zhonghua Bing Li Xue Za Zhi 22(4): 211-213

Using immunohistochemical and Northern blot hybridization techniques, the expression of epidermal growth factor (EGF) and its mRNA was studied in 3 human pancreatic carcinoma cell lines and their in vivo tumor lines. 2 out of 3 cell lines (PC-2 and PC-3) showed strong immunoreaction with EGF antiserum whereas PC-1 was stained weakly. Northern blot exhibited expression of EGF mRNA in PC-2 and PC-3. No EGF mRNA was detected in PC-1. The detectable levels of EGF protein and EGF mRNA in this study and EGF receptor mRNA in our previous study, indicated the presence of autocrine cycle in human pancreatic carcinoma cell lines. The autostimulation of growth factor to cancer cells and overexpression of growth factor receptors may be the main mechanism of uncontrolled growth of cancer cells.


PMID: 8168178

Other references

Kozlowski, M., 1973: A review of some methods for preservation of potatoes. Nowe rolnictwo: 15, 22 (13) 18-19

Piccoli, G.; Segoloni, G.; Stratta, P.; Dogliani Stratta, M.; Coppo, R.; Ragni, R.; Vercellone, A., 1973: Use of information computers in the clinical management of the periodic hemodialysis treatment. Minerva Nefrologica 20(6): 342-349

Thomann, K.H.; Dul, M.W., 1994: Bone scintigraphy: a review of the procedure and its applications. Bone scintigraphy (bone scanning) is a sensitive diagnostic tool used to detect the presence and extent of primary and secondary bone disease. Unlike X-rays, MRI, and CT scans, which focus primarily on structural anatomy, bone scans depict the dyn...

Ciriolo, M.R.; Aducci, P.; Battistoni, A.; Carri, M.T.; Civitareale, P.; D.M.rtino, A.; Galiazzo, F.; Marra, M.; Nasta, D.; Rotilio, G., 1994: Regulation of Cu,ZnSOD by metals in S cerevisiae and Z mays. Asada, K , Yoshikawa, T International Congress Series; Frontiers of reactive oxygen species in biology and medicine 285-286

O'Doherty, J.; Henricson, J.; Anderson, C.; Leahy, M.J.; Nilsson, G.E.; Sjöberg, F., 2007: Sub-epidermal imaging using polarized light spectroscopy for assessment of skin microcirculation. Many clinical conditions that affect the microcirculation of the skin are still diagnosed and followed up by observational methods alone in spite of the fact that non-invasive, more user-independent and objective methods are available today. Limit...

Sahlberg, John., 1913: Till kannedomen om Haltica engstroemi och dess biologi.. Entomologisk Tidskrift Stockholm, 34 261-270

Qu, M.; Tong, C.; Kong, L.; Yan, X.; Chernikov, O.V.; Lukyanov, P.A.; Jin, Q.; Li, W., 2015: Purification of a secreted lectin from Andrias davidianus skin and its antibacterial activity. A lectin secreted from Andrias davidianus skin (ADL) was purified by affinity chromatography on porcine stomach mucin (type III) (PSM)-crosslinked albumin, followed by gel filtration on Sephadex G-100 and HPLC on TSK gel G3000PWXL. The purified le...

Bobrov, V.M.; Shishkin, S.A., 1999: Middle-mass molecules as an indicator of intoxication in ENT pyoinflammatory diseases. Middle-mass molecules (MMM) were measured in ENT patients hospitalized urgently for purulent sinusitis with orbital complications, mastoiditis, parapharyngitis, paratonsillar abscesses, complicated furuncles of the nose and upper lip, abscessing e...

Gullino, P.M.; Lanzerotti, R.H., 1972: Mammary tumor regression. II. Autophagy of neoplastic cells. Journal of the National Cancer Institute 49(5): 1349-1356

Kim, S.H.; Engelhardt, J.I.; Henkel, J.S.; Siklós, L.; Soós, J.; Goodman, C.; Appel, S.H., 2004: Widespread increased expression of the DNA repair enzyme PARP in brain in ALS. Expression of the DNA repair enzyme poly(ADP-ribose) polymerase (PARP) is a known response to oxidative damage of DNA. In ALS brain, PARP expression by western analyses was increased in the motor cortex, parietal cortex, and cerebellum. PARP immun...