+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Expression of TRAIL (Apo2L), DR4 (TRAIL receptor 1), DR5 (TRAIL receptor 2) and TRID (TRAIL receptor 3) genes in multidrug resistant human acute myeloid leukemia cell lines that overexpress MDR 1 (HL60/Tax) or MRP (HL60/AR)

, : Expression of TRAIL (Apo2L), DR4 (TRAIL receptor 1), DR5 (TRAIL receptor 2) and TRID (TRAIL receptor 3) genes in multidrug resistant human acute myeloid leukemia cell lines that overexpress MDR 1 (HL60/Tax) or MRP (HL60/AR). International Journal of Oncology 16(6): 1137-1139

Previously we have reported a differential expression of CD95/CD95L and Bcl-2 family of genes in multidrug resistant tumor cells. TRAIL, a member of the TNF receptor family, induces apoptosis in many tumor cells by binding to DR4 (TRAIL receptor 1) and DR5 (TRAIL receptor 2). In contrast, TRAIL-induced apoptosis is prevented by a decoy receptor (DcR1, TRID or TRAIL receptor 3). In the present study, we compared the expression of TRAIL, DR4, DR5, and TRID between a drug sensitive HL60, a myeloid leukemia cell line, and its multidrug resistant (MDR) sublines that either overexpressed MDR 1 gene (HL60/Tax) or MRP gene (HL60/AR), using RT-PCR. TRAIL mRNA was expressed in HL60 cells but was present in low levels in HL60/AR cells and was completely lacking in HL60/Tax cells. Both DR4 and DR5 were undetectable in HL60/Tax but were present at comparable levels in HL60/AR and drug sensitive HL60 cells. TRID were absent in HL60 and HL60/Tax cells, but was present in low but comparable levels in peripheral blood mononuclear cells and HL60/AR cells. These data suggest that the multidrug resistance in MDR HL60 cell lines, regardless of overexpression of MDR 1 or MRP, may be due to different mechanisms. In HL60/AR cells it appears that MDR may be due to decreased expression of TRAIL and constitutive expression of TRID, whereas in HL60/Tax cells, MDR could be due to the absence of TRAIL and/or DR4 and DR5.


PMID: 10811986

Other references

Starr, C.; Taggart, R., 1995: Biology the unity diversity of life 7th edition Part 6 Animal structure and function. Unknown

Chowdhury,H.; Banerjee,T.; Walia,S., 2008: In vitro screening of Curcuma longa L and its derivatives as antifungal agents against Helminthosporium oryzae and Fusarium solani. <i>Curcuma longa</i> (turmeric) based products namely curcumins I, II, III, alkyl ether derivatives of curcumin-I and turmeric oil were evaluated for their antifungal activity against two phytophagous fungi <i>Helminthosporium or...

Marechal, G.; Aubert, X., 1959: Analysis of isometric contraction of isolated striated muscle in various types of fatigue tests. Journal de Physiologie 51(3): 527-527

Amdur B.H.; Brudevold F.; Tehrani A., 1983: The effect of enamel de mineralization and re mineralization on permeability to iodide and urea. Blocks of bovine enamel subjected to de- and remineralization were measured for changes in permeability to iodide and urea. Results were similar, indicating that the diffusion pathways for the 2 species were comparable, and that sites for iodide b...

Proches,S.; Warren,M.; McGeoch,M.A.; Marshall,D.J., 2010: Spatial scaling and transition in pneumatophore arthropod communities. Although most ecological variables are scale-dependent, few studies cover a broad range of spatial scales. Here, we consider South African mangrove pneumatophore arthropod communities (mites, crustaceans and insects), across seven spatial scales (...

van Wijk, D.; Cohet, E.; Gard, A.; Caspers, N.; van Ginkel, C.; Thompson, R.; de Rooij, C.; Garny, V.; Lecloux, A., 2005: 1,2,4-trichlorobenzene marine risk assessment with special emphasis on the Osparcom region North Sea. A risk assessment on 1,2,4-trichlorobenzene was carried out specifically for the marine environment according to the methodology laid down in the EU Risk Assessment Regulation 1488/94 and the Guidance Documents of the EU Existing Substances Regula...

Wang DeLi; L.X.nLong; Zhu Ling, 1996: Analysis to influences of grazing on natural rangelands in Hulunbeir, China. Studies of sward botanical composition, plant succession and biomass production carried out at Modamuji, Nei Menggu, China across a 10-km area grazed by herded sheep are described. The climax vegetation was Leymus chinensis and Stipa grandis but t...

Dempfle, L., 1984: Principles of estimation of breeding values. Three aspects of the progeny testing of bulls for dairy performance are discussed: (1) correction for systematic and random effects on performance; (2) extraction of the max. possible amount of information from the data available; (3) making use o...

Satone, H.; Lee, J.Man.; Oba, Y.; Kusakabe, T.; Akahoshi, E.; Miki, S.; Suzuki, N.; Sasayama, Y.; Nassef, M.; Shimasaki, Y.; Kawabata, S-Ichiro.; Honjo, T.; Oshima, Y., 2011: Tributyltin-binding protein type 1, a lipocalin, prevents inhibition of osteoblastic activity by tributyltin in fish scales. Tributyltin-binding protein type 1 (TBT-bp1) is a member of the lipocalin family of proteins which bind to small hydrophobic molecules. In this study, we expressed a recombinant TBT-bp1 (rTBT-bp1, ca. 35kDa) in a baculovirus expression system and...

Prichard T.L., 1992: Volume balance approach to quality winegrape irrigation. American Journal of Enology & Viticulture 43(3): 303