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Expression of TRAIL (Apo2L), DR4 (TRAIL receptor 1), DR5 (TRAIL receptor 2) and TRID (TRAIL receptor 3) genes in multidrug resistant human acute myeloid leukemia cell lines that overexpress MDR 1 (HL60/Tax) or MRP (HL60/AR)


, : Expression of TRAIL (Apo2L), DR4 (TRAIL receptor 1), DR5 (TRAIL receptor 2) and TRID (TRAIL receptor 3) genes in multidrug resistant human acute myeloid leukemia cell lines that overexpress MDR 1 (HL60/Tax) or MRP (HL60/AR). International Journal of Oncology 16(6): 1137-1139

Previously we have reported a differential expression of CD95/CD95L and Bcl-2 family of genes in multidrug resistant tumor cells. TRAIL, a member of the TNF receptor family, induces apoptosis in many tumor cells by binding to DR4 (TRAIL receptor 1) and DR5 (TRAIL receptor 2). In contrast, TRAIL-induced apoptosis is prevented by a decoy receptor (DcR1, TRID or TRAIL receptor 3). In the present study, we compared the expression of TRAIL, DR4, DR5, and TRID between a drug sensitive HL60, a myeloid leukemia cell line, and its multidrug resistant (MDR) sublines that either overexpressed MDR 1 gene (HL60/Tax) or MRP gene (HL60/AR), using RT-PCR. TRAIL mRNA was expressed in HL60 cells but was present in low levels in HL60/AR cells and was completely lacking in HL60/Tax cells. Both DR4 and DR5 were undetectable in HL60/Tax but were present at comparable levels in HL60/AR and drug sensitive HL60 cells. TRID were absent in HL60 and HL60/Tax cells, but was present in low but comparable levels in peripheral blood mononuclear cells and HL60/AR cells. These data suggest that the multidrug resistance in MDR HL60 cell lines, regardless of overexpression of MDR 1 or MRP, may be due to different mechanisms. In HL60/AR cells it appears that MDR may be due to decreased expression of TRAIL and constitutive expression of TRID, whereas in HL60/Tax cells, MDR could be due to the absence of TRAIL and/or DR4 and DR5.

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PMID: 10811986


Other references

Starr, C.; Taggart, R., 1995: Biology the unity diversity of life 7th edition Part 6 Animal structure and function. Unknown

Chowdhury,H.; Banerjee,T.; Walia,S., 2008: In vitro screening of Curcuma longa L and its derivatives as antifungal agents against Helminthosporium oryzae and Fusarium solani. <i>Curcuma longa</i> (turmeric) based products namely curcumins I, II, III, alkyl ether derivatives of curcumin-I and turmeric oil were evaluated for their antifungal activity against two phytophagous fungi <i>Helminthosporium or...

Marechal, G.; Aubert, X., 1959: Analysis of isometric contraction of isolated striated muscle in various types of fatigue tests. Journal de Physiologie 51(3): 527-527

Amdur B.H.; Brudevold F.; Tehrani A., 1983: The effect of enamel de mineralization and re mineralization on permeability to iodide and urea. Blocks of bovine enamel subjected to de- and remineralization were measured for changes in permeability to iodide and urea. Results were similar, indicating that the diffusion pathways for the 2 species were comparable, and that sites for iodide b...

Proches,S.; Warren,M.; McGeoch,M.A.; Marshall,D.J., 2010: Spatial scaling and transition in pneumatophore arthropod communities. Although most ecological variables are scale-dependent, few studies cover a broad range of spatial scales. Here, we consider South African mangrove pneumatophore arthropod communities (mites, crustaceans and insects), across seven spatial scales (...

van Wijk, D.; Cohet, E.; Gard, A.; Caspers, N.; van Ginkel, C.; Thompson, R.; de Rooij, C.; Garny, V.; Lecloux, A., 2005: 1,2,4-trichlorobenzene marine risk assessment with special emphasis on the Osparcom region North Sea. A risk assessment on 1,2,4-trichlorobenzene was carried out specifically for the marine environment according to the methodology laid down in the EU Risk Assessment Regulation 1488/94 and the Guidance Documents of the EU Existing Substances Regula...

Wang DeLi; L.X.nLong; Zhu Ling, 1996: Analysis to influences of grazing on natural rangelands in Hulunbeir, China. Studies of sward botanical composition, plant succession and biomass production carried out at Modamuji, Nei Menggu, China across a 10-km area grazed by herded sheep are described. The climax vegetation was Leymus chinensis and Stipa grandis but t...

Dempfle, L., 1984: Principles of estimation of breeding values. Three aspects of the progeny testing of bulls for dairy performance are discussed: (1) correction for systematic and random effects on performance; (2) extraction of the max. possible amount of information from the data available; (3) making use o...

Satone, H.; Lee, J.Man.; Oba, Y.; Kusakabe, T.; Akahoshi, E.; Miki, S.; Suzuki, N.; Sasayama, Y.; Nassef, M.; Shimasaki, Y.; Kawabata, S-Ichiro.; Honjo, T.; Oshima, Y., 2011: Tributyltin-binding protein type 1, a lipocalin, prevents inhibition of osteoblastic activity by tributyltin in fish scales. Tributyltin-binding protein type 1 (TBT-bp1) is a member of the lipocalin family of proteins which bind to small hydrophobic molecules. In this study, we expressed a recombinant TBT-bp1 (rTBT-bp1, ca. 35kDa) in a baculovirus expression system and...

Prichard T.L., 1992: Volume balance approach to quality winegrape irrigation. American Journal of Enology & Viticulture 43(3): 303