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Hyaluronan synthesis by epiphysial chondrocytes is regulated by growth hormone, insulin-like growth factor-1, parathyroid hormone and transforming growth factor-beta 1

, : Hyaluronan synthesis by epiphysial chondrocytes is regulated by growth hormone, insulin-like growth factor-1, parathyroid hormone and transforming growth factor-beta 1. Matrix Biology 15(6): 423-432

In a previous study, we presented evidence that the synthesis of hyaluronan by hypertrophic chondrocytes is one of the principal factors driving the interstitial expansion of the growth plate (Pavasant et al., J. Cell Sci. 109: 327-334, 1996). To test this possibility further, we used two different approaches to examine the effects of hormones on the production of hyaluronan in the growth plate. In the first approach, we examined the growth plate of the lit/lit mouse that lacks growth hormone and found that its hypertrophic lacunae were smaller and contained less hyaluronan than those of wild type mice. Moreover, the ratios of hyaluronan staining density to total area of the lacunae were similar for the lit/lit and the wt/wt mice, indicating that the amount of hyaluronan is directly related to lacuna size. In the second approach, we examined the effects of hormones on segments of the epiphysial growth plate placed in organ culture. Under normal culture conditions, a band of hyaluronan staining progressed across the length of the growth plate, reflecting the maturation of chondrocytes into the hypertrophic stage. When insulin-like growth factor-1, a factor known to promote chondrocyte maturation, was added to the culture medium, the production of hyaluronan and the enlargement of the lacunae were stimulated. In contrast, when either parathyroid hormone or transforming growth factor-beta 1, both of which inhibit chondrocyte differentiation, was added to the medium of cultured segments, new pericellular hyaluronan was not detected and the lacunae did not enlarge. Taken together, these results indicate that factors that either stimulate or inhibit the maturation of epiphysial chondrocytes have a corresponding effect on the production of hyaluronan. This, in turn, further supports the importance of hyaluronan in the process of lacuna enlargement.


PMID: 9049980

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Meng, X.; Lv, S.; Cheng, D.; Hu, Q.; Ma, J.; Liu, B.; Hu, Y., 2017: Fused Multifunctionalized Chromenes from Tetraynes and α,β-Unsaturated Aldehydes. The synthesis of fused, highly substituted chromenes in a one-pot-three-step cascade hexadehydro-Diels-Alder reaction of tetraynes followed by an intermolecular α,β-unsaturated aldehyde rearrangement is reported. The target compounds were prepar...

Klepper, R.; Kuhne-Velte, H.; Kaufmann, J., 1989: Rotational irradiation with a centrally placed block. A rotational field technique with a central placed block generates nearly circular isodoses with a pronounced minimum in its center, comparable with a cone-shaped mountain with a crater. The shape of both, mountain and crater, can be effectively v...

Sigleo A.C.; Hoering T.C.; Hare P.E., 1981: The colloidal organic matter in waters of the chesapeake bay and patuxent river usa. Bowers, R (Ed ) Carnegie Institution Of Washington Year Book, 1979 Viii+697p Carnegie Institution Of Washington: Washington, D C , Usa Illus P394-399, 1979, 1980

Mayne, J.G.; Martin, M.J.; Morrow, G.W.; Turner, R.M.; Hisey, B.L., 1969: A health questionnaire based on paper-and-pencil medium individualized and produced by computer. I. Technique. JAMA 208(11): 2060-2063

Schmitt, H.E., 1970: Hematoma of the stomach wall after blunt abdominal injury. Fortschritte auf dem Gebiete der Rontgenstrahlen und der Nuklearmedizin 113(1): 114-115

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Loschen, G.; Azzi, A.; Richter, C.; Flohé, L., 1974: Superoxide radicals as precursors of mitochondrial hydrogen peroxide. Febs Letters 42(1): 68-72

Fischer, B., 1947: Treatment of class II, division I (angle) occipital anchorage. American Journal of Orthodontics 33(11): 705-737

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