geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Identification and characterization of the UL14 gene product of herpes simplex virus type 2


, : Identification and characterization of the UL14 gene product of herpes simplex virus type 2. Journal of General Virology 80: 2423-2431

The UL14 gene of herpes simplex virus type 2 (HSV-2) is predicted to encode a 219 amino acid protein with a molecular mass of 23 kDa. In this study, the HSV-2 UL14 gene product has been identified by using a rabbit polyclonal antiserum raised against a recombinant 6 x His-UL14 fusion protein expressed in E. coli. The antiserum reacted specifically with 34, 33 and 28 kDa proteins in HSV-2-infected cell lysates and also with a 34 kDa protein produced by in vitro transcription and translation reactions, suggesting that the 34 kDa protein is the primary translation product of the UL14 gene. The protein was synthesized at late times post-infection (p.i.) and was not detectable in the presence of the viral DNA synthesis inhibitor acycloguanosine. Indirect immunofluorescence studies localized the UL14 protein both to the nucleus and to perinuclear regions of the cytoplasm, and the nuclear UL14 protein was found to co-localize with the scaffolding protein ICP35 at 9 h p.i. However, the protein accumulated in a perinuclear region of the cytoplasm at 12 h p.i., while most of the ICP35 protein localized within assemblons in the nucleus. Although no detectable UL14 protein was associated with intracellular capsids isolated in the presence of 0.5 M NaCl, it was detected in purified virions. Furthermore, the UL14 protein expressed alone was detected both in the nucleus and in the cytoplasm at 24 h after transfection, but was mainly localized to the cytoplasm at later times.

US$19.90

PMID: 10501497

DOI: 10.1099/0022-1317-80-9-2423


Other references

Cypreste, R.; Walsh, K.; Bedford, M., 2016: Evidence-based medicine: what does the future hold?. Postgraduate Medical Journal 91(1077): 359-360

Bosch Del Marco, L.M., 1952: Lymphatic cyst of the mesentery causing an intestinal occlusion syndrome. Archivos Uruguayos de Medicina, Cirugia Y Especialidades 41(1-2): 82-86

Petsko, G.A., 2012: The blue marble. Genome Biology 12(4): 112-112

Kenny, P.J., 1968: Reconstructive surgery in postgastrectomy states. Australian and New Zealand Journal of Surgery 37(3): 264-275

Goard, C.A.; Schimmer, A.D., 2013: An evidence-based review of obatoclax mesylate in the treatment of hematological malignancies. Obatoclax mesylate is an intravenously-administered drug under investigation in Phase I and II clinical trials as a novel anticancer therapeutic for hematological malignancies and solid tumors. Obatoclax was developed as a pan-inhibitor of antiapo...

Zhumabayeva, B.; Chenchik, A.; Siebert, P.D., 1999: RecA-mediated affinity capture: a method for full-length cDNA cloning. We describe an improved method for rapid cloning of full-length cDNA from cDNA libraries. This approach is based on the ability of Escherichia coli RecA protein to form a stable nucleoprotein complex with a linear single-stranded DNA probe and hom...

Helke, C.J.; Eskay, R.L., 1985: Capsaicin reduces substance P immunoreactivity in the lateral nucleus of the solitary tract and nodose ganglion. The effects of the neonatal administration of capsaicin on substance P (SP) immunoreactivity of the vagal sensory ganglion (nodose ganglion) and the CNS site of termination of vagal afferents (nucleus of the solitary tract-NTS) were evaluated with...

Convey, E.M.; Beck, T.W.; Neitzel, R.R.; Bostwick, E.F.; Hafs, H.D., 1976: Negative feedback control of bovine serum luteinizing hormone (LH) concentration from completion of the preovulatory LH surge until resumption of luteal function. The objective of the experiment was to determine if ovarian hormone feedback on LH secretion is operative from completion of the preovulatory LH surge to resumption of luteal function in cattle, i.e. when serum progesterone concentrations are <...

Ajimilah Nyak Husain, 1984: Quality parameters for Malaysian rice varieties. Analytic data from 10 non glutinous and 2 glutinous local varieties are explained and tabulated. Characters measured were (a) grain weight, shape and colour; (b) milling quality, amylose content, gelatinization temperature and gel consistency; (c)...

Panchenko, S.M., 2000: Huperzia selago (L.) Bernh. ex Schrank and Mert. (Huperziaceae Rothm.) clones' particularities in the North Eastern part of Ukraine. Eleven clones of Huperzia selago (L.) Bernh. ex Schrank and Mert. (Huperziaceae) were studied in the North-Eastern part of Ukraine (Sumy region). The morphometric analysis of all clones has been carried out. Output discreptancy between vegetative...