+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Induction of apoptosis by JTE-522, a specific cyclooxygenase-2 inhibitor, in human gastric cancer cell lines

, : Induction of apoptosis by JTE-522, a specific cyclooxygenase-2 inhibitor, in human gastric cancer cell lines. Anticancer Research 20(6b): 4279-4284

An increased expression of cyclooxygenase (COX)-2 has been observed in various cancers including gastric cancer. Although specific COX-2 inhibitors have a chemopreventive effect on colon cancer, their molecular mechanisms remain unclear. To clarify these mechanisms, we investigated the effects of JTE-522, a newly developed COX-2-specific inhibitor, on gastric cancer cell lines (MKN28 and MKN45). The baseline levels of COX-2 expression were higher in MKN45 than in MKN28. JTE-522 obviously suppressed the levels of COX-2 mRNA, COX-2 protein and PGE2 at a dose of 250 microM in both cancer cells. Apoptosis was induced at 24 hours after treatment with JTE-522 (250 microM) in both cancer cells. To determine the mechanisms of apoptosis induction by JTE-522, the time course of the cell cycle and the apoptosis-related protein levels were examined. An increase in the G1 phase and a decrease in the S phase were observed prior to apoptosis. Moreover, an increase of c-myc protein and a decrease of bcl-2 protein were observed in both cells treated with JTE-522. These findings suggested that JTE-522 could induce apoptosis by blocking the cell cycle, enhancing c-myc expression and diminishing bcl-2 expression. JTE-522 also suppressed proliferation activity in both cell lines. These effects of JTE-522 were more dramatic in MKN45 than in MKN28. Since JTE-522 strongly suppresses cell growth by inducing apoptosis in gastric cancer cell lines, it may therefore serve as a chemopreventive agent.


PMID: 11205258

Other references

Aubert, M.A.angana, Q., 1996: Self-potential method in hydrogeological exploration of volcanic areas. In volcanic areas, when two conditions are satisfied, there is a linear correlation between the range of negative self-potential (SP) anomalies and the thickness of the unsaturated zone measured in borings. The first condition is a high ratio betw...

Chaves, Miguel M., 1960: On the presence of alkaloids in the roots of leiteiro. The plant is a toxic weed and difficult to eradicate. In carrying out the study on its alkaloids, comparisons were made with Rauwolfia sp. Tests included precipitation reactions, coloration tests and paper chromatog-raphy, and results suggested th...

Hansen K., 1984: Discrimination and production of disparlure enantiomers by the gypsy moth and the nun moth. Two odor receptor cells were physiologically identified within male antennal hair sensillae of the gypsy moth, Lymantria disper L. and the nun moth, L. monacha L. In the gypsy moth, one cell responded to (+)-disparlure, while a neighboring cell re...

Krauth, K., 1991: Increase of the biomass through ultrafiltration with aerobic and anaerobic operated bioreactors. An ultrafiltration separation process was used to increase the biomass in aerobic and anaerobic waste water treatment processes, in order to improve their yield. Biomass of up to 25 g/litre were achieved, with a speed of about 6 m/s in a polysulph...

Katamine, S.; Kurokawa, K.; Miyamoto, T., 1997: Maternal transmission of human T-cell lymphotropic virus type I (HTLV-I): Intervention in an endemic area (Nagasaki, Japan). Human T-cell lymphotropic virus type I (HTLV-I) is a causative agent of a type of leukaemia, known as adult T-cell leukaemia (ATL). We identified milk-borne transmission as the major pathway for maternal infection of HTLV-I by epidemiological stud...

Ramdohr P., 1972: The highly reflecting and opaque components in the mineral content of the Haveroe meteorite. Observations, comparison with Novo Urei ureilite, southwestern Finland.

Sutton B.C., 1971: Coelomycetes part 4 the genus harknessia and similar fungi on eucalyptus d. Commonwealth Mycological Institute Mycological Papers (123): 1-46

Kaiser Wilhelm-Gesellschaft., 1942: Report on the activities of the Kaiser Wilhelm Gesellschaft for the Promotion of Science in 1941-42. AT the Kaiser Wilhelm Institute for Animal Breeding, Rostock-Dummerstorf, investigations were made into the following subjects: crossing Schleswig and Rhineland cold-blood horses; crossing of Jersey with Black Pied Lowland cattle to raise the milk...

Guillin, M.C., 1994: Prolonged activated partial thromboplastin time, Quick's time, bleeding time. Diagnostic orientation. La Revue du Praticien 44(14): 1942-1948

Huber, W.; Marquard, E., 1985: Plasma potassium and blood pH following physical exercise in dialysis patients. Nephron 40(3): 383-384