geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Induction of the c-jun protooncogene expression by areca nut extract and arecoline on oral mucosal fibroblasts


, : Induction of the c-jun protooncogene expression by areca nut extract and arecoline on oral mucosal fibroblasts. Oral Oncology 36(5): 432-436

To investigate the mechanisms of areca quid (AQ)-induced carcinogenesis, expression of c-fos and c-jun protooncogenes was examined in human oral mucosal fibroblasts after exposure to areca nut extracts (ANE) or arecoline. We found that treatment of cells with 200 microg/ml ANE or 10 microg/ml arecoline for 1 h induced about three-fold increase in c-jun mRNA levels. This increase was transient and the level of c-jun mRNAs returned rapidly to control levels thereafter. However, ANE and arecoline did not induce c-fos mRNA expression at detectable levels. During AQ chewing, oral mucosal cells are continuously stimulated by ANE and arecoline. Persistent induction of the c-jun protooncogene by ANE and arecoline may be one of the mechanisms in the carcinogenesis of oral squamous cell carcinoma in Taiwan. Furthermore, we observed that pre-incubation of cells with either N-acetyl-cysteine [a glutathione (GSH) precursor] or L-buthionine-S,R-sulfoximine (a specific inhibitor of GSH biosynthesis) had a minimal effect on arecoline-induced c-jun expression. Therefore, arecoline-induced c-jun expression is independent of GSH depletion.

US$29.90

PMID: 10964049


Other references

Cocke, W.M.; Bolasny, B.L.; Sawyers, J.L., 1970: Vaginal reconstruction folowing extended abdominoperineal resection. Plastic and Reconstructive Surgery 46(4): 372-374

Bramstedt, F., 1940: Bornerina, a new genus of the Atheroididae (Chaetophoridae).. Description of Bornerina depressa, a new genus and species of aphid found on Alnus viridis in Austria.

Hine, R.; Cloud, H.; Carithers, T.H.ckey, C.H.nton, A., 1990: Early nutrition intervention services for children with special health care needs. Dietitians must be responsive to the changing needs of their clients and employers, to societal concerns, and to legal mandates. A recently passed amendment (PL 99-457) to the Federal Education for the Handicapped Act gives nutrition professionals...

Nihoyannopoulos, P., 2001: Tricuspid valvectomy following tricuspid valve endocarditis on an intravenous drug addict. Heart 86(2): 144-144

Hasle, H.; Rasmussen, H., 1988: Unrecognized occupational diseases among unskilled workers having chosen early retirement. Ugeskrift for Laeger 150(38): 2253-2255

Zou, Y.; Mei, M., 1997: Effects of hepatocyte growth factor on dose- and time-response of DNA synthesis in rat hepatocytes in vitro. Effects of recombinant human hepatocyte growth factor (r-hHGF) on dose- and time-response of DNA synthesis were observed by using the method of 3H-TdR incorporation into DNA in rat hepatocytes in vitro. The results showed that r-hHGF was the most...

Goffin, Y., 1968: Postmortem variations and effect of autolysis on some hydrolytic enzymes of the skin and skin appendages. Acta Pathologica et Microbiologica Scandinavica 73(3): 351-358

Adam, L.; Ellwood, S.; Wilson, I.; Saenz, G.; Xiao, S.; Oliver, R.P.; Turner, J.G.; Somerville, S., 2000: Comparison of Erysiphe cichoracearum and E. cruciferarum and a survey of 360 Arabidopsis thaliana accessions for resistance to these two powdery mildew pathogens. In previous work, UEA1 and UCSC1, two geographically distinct, powdery mildew isolates, were recognized for their ability to infect Arabidopsis thaliana. We have clarified the identity of these isolates by determining their host ranges, reexaminin...

Holubec, V.; Kuhn, F.; Sevcikova, M.; Zapletalova, I., 1994: Requirements for collection and conservation of wild genetic resources and landraces of cultivated plants and the 1993 expedition in the White Carpathians. During a collecting mission in this region of the Czech Republic, 250 accessions were collected. Larger samples were placed directly in the gene bank, while smaller amounts of seed from all samples were retained for multiplication in specialized c...

Quoidbach, A., 1967: Study of body composition by isotopic dissection. I. General principles and clinical applications. Bulletin der Schweizerischen Akademie der Medizinischen Wissenschaften 23(3): 196-206