geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Influence of hemodialysis membranes on gene expression and plasma levels of interleukin-1 beta


, : Influence of hemodialysis membranes on gene expression and plasma levels of interleukin-1 beta. Artificial Organs 19(8): 842-846

Plasma levels of interleukin-1 beta (IL-1 beta) were measured in 10 normal subjects, in 11 nondialyzed end-stage renal failure (ESRD) patients, and in 22 hemodialysis (HD) patients. Of the HD patients, 7 were dialyzed with Cuprophan (CU), 7 with polymethylmethacrylate (PMMA), and 8 with polysulphone (PS) dialyzers. In normal controls, nondialyzed ESRD patients, and HD equipped with CU, PAMM, and PS dialyzers, plasma levels of IL-1 beta were 10.73 +/- 5.24 pg/ml, 9.97 +/- 3.61 pg/ml, 13.17 +/- 4.04 pg/ml, 15.16 +/- 6.16 pg/ml, and 13.96 +/- 5.47 pg/ml, respectively. There were no statistically significant differences among the groups (p > or = 0.05). In contrast, the gene expression of IL-1 beta for peripheral blood mononuclear cells (PBMC) by in situ hybridization showed differences among the groups. The gene for IL-1 beta for PBMC appears in HD equipped with different membranes, but not in cases of nondialyzed uremic patients and normal subjects. With computer imaging analysis, we carried out quantitative analysis of cells in in situ hybridization with an area of positive spots to an area of total cells. In HD with CU, PMMA, and PS, the results were 10.64 +/- 1.07, 3.34 +/- 0.74, and 3.27 +/- 0.64%, respectively. The levels of IL-1 beta gene expression in CU were higher than that in PMMA or PS. There were statistically significant differences (p < or = 0.001) between CU and PMMA or PS and no significant difference between the PMMA and PS (p > or = 0.05). We suggest measuring the gene expression of cytokines for PBMC and which may be better than measuring cytokine levels only for investigating the blood compatibility of dialyzers, which may help in understanding chronic complications of the dialysis procedure.

US$29.90

PMID: 8573006


Other references

Veitch, R., 1932: Report of the Chief Entomologist. The scarcity of Dacus ferrugineus, F. (Chaetodacus tryoni, Frogg.) in the Stanthorpe district of Queensland during the year 1931-32 is attributed chiefly to increased care in orchard sanitation, the destruction of the adults by baits, and the occu...

Nouchi, R.; Taki, Y.; Takeuchi, H.; Sekiguchi, A.; Hashizume, H.; Nozawa, T.; Nouchi, H.; Kawashima, R., 2014: Four weeks of combination exercise training improved executive functions, episodic memory, and processing speed in healthy elderly people: evidence from a randomized controlled trial. Previous reports have described that long-term combination exercise training improves cognitive functions in healthy elderly people. This study investigates the effects of 4 weeks of short-term combination exercise training on various cognitive f...

Lichtenstein, J.E.; Fitzpatrick, J.J.; Madewell, J.E., 1988: The role of radiology in fatality investigations. During the last few years streptococcal infections have been reported in connection with various cutaneous manifestations with severe or fatal outcome. A 62-year-old female with chronic interstitial nephritis was admitted with progression of confl...

Liu, X.; Yu, L.; Primak, A.N.; McCollough, C.H., 2009: Quantitative imaging of element composition and mass fraction using dual-energy CT: three-material decomposition. In principle, dual-energy CT can only accurately decompose a mixture into two materials. To decompose a mixture into three constitute materials using dual-energy CT measurements, a third criteria must be provided to solve for three unknowns with o...

Anonymous, 1972: Prevention of bacterial endocarditis. American Heart Association. Journal of the American Dental Association 85(6): 1377-1379

Moon, K-Ho.; Kim, Y-Tae., 2014: Medical Treatment of Ankylosing Spondylitis. The diagnosis of ankylosing spondylitis is often delayed due to ambiguous clinical manifestations and strict diagnostic criteria. However, imaging techniques such as magnetic resonance imaging have been found effective for the early diagnosis of n...

Madrid, A.H.; Moro, C.; Chadli, A.; Garcia, E.; Catalan, P.; Alonso, L., 1996: Correlation of QT dispersion and late potentials and inducibility at the electrophysiology laboratory. European Heart Journal 17(ABSTR SUPPL ): 124

Oseroff A.R.; Wadwa K.; Ohuoha D.; Dahl T.; Ara G., 1989: The malignant cell selective phototoxicity of cationic dyes is synergistically enhanced by mild hyperthermia. Proceedings of the American Association for Cancer Research Annual Meeting 30: 550

Ikeda, M.; Takeuchi, M.; Shinkai, S., 2003: Unusual emission properties of a triphenylene-based organogel system. We have found that compound 1 forms organogels in appropriate organic solvents and the resultant gel phase exhibits unusual emission properties arising from the excimer formation.

Ganesh, M.J.; Law, S.E., 1988: Environmental and electrical effects upon levitation in traveling electric-field conveyance of agricultural particulates. Concepts and devices were developed for agricultural materials handling by the direct application of electric force fields. An electric curtain apparatus for levitating agricultural particulates driven by a single-phase a.c. source is described. L...