+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Interactions of pentosan polysulfate with cartilage matrix proteins and synovial fibroblasts derived from patients with osteoarthritis

, : Interactions of pentosan polysulfate with cartilage matrix proteins and synovial fibroblasts derived from patients with osteoarthritis. Osteoarthritis and Cartilage 4(1): 43-53

Pentosan polysulfate (PPS) has been shown to improve symptoms of patients with osteoarthritis (OA) when studied under double-blinded conditions. Laboratory studies indicated that this drug exhibits multiple actions, including the preservation of articular cartilage (AC) proteoglycans in animal models of OA and the stimulation of hyaluronan synthesis by synovial fibroblasts in vitro and in vivo. As PPS is strongly anionic and has a molecular weight of approximately 5700 Da its ability to enter connective tissues rich in proteoglycans and interact with the resident cells has been questioned. In the present studies, experiments were undertaken to isolate and characterize proteins in human AC which have the potential to bind PPS. Thrombospondin was identified in 4.0 M GuHCl extracts of human AC as a PPS-binding protein. Furthermore, synovial fibroblasts derived from OA joints were shown to secrete thrombospondin and also bind PPS. Using bovine erythrocytes conjugated with PPS a rosetting of the synovial fibroblast could be demonstrated. The level of rosetting was not affected by pre-incubating cultures with thrombospondin antibody suggesting that PPS was interacting directly with the cells. Kinetic studies of 3H-PPS uptake by synovial fibroblasts showed saturation of binding sites within 30 min when cells were maintained at 4 degrees C but preservation of drug uptake for up to 120 min when cells were cultured at 37 degrees C. These data, together with the finding that cells labeled with drug at 37 degrees C showed higher incorporation, than at 4 degrees C after trypsin digestion suggests that PPS first binds to the cell membrane when at 37 degrees C is internalized, possibly by pinocytosis.


PMID: 8731395

Other references

F.Bartolucci, G.E.Montanari, S.Pandolfi, 2012: Dimensionality of the Latent Structure and Item Selection Via Latent Class Multidimensional IRT Models. With reference to a questionnaire aimed at assessing the performance of Italian nursing homes on the basis of the health conditions of their patients, we investigate two relevant issues: dimensionality of the latent structure and discriminating po...

Tonchev, A.; Stankulov, I.; Ghenev, P.; Pavlov, P.; Chaldakov, G.; Yamashima, T., 2006: Identification of putative progenitor cells in the anterior subventricular zone of adult human brain. The subventricular zone along the anterior horn (SVZa) of the lateral ventricle in the brain contains progenitor cells which are capable of neuronal generation in adulthood in a process referred to as adult neurogenesis. Despite the significant re...

Renkhoff, Martin., 1992: Wird die Englische Schafstelze (Motacilla f. flavissima) beim Durchzug ubersehen?. Charadrius, 284: 199-200

Kadono, T.; Shiraki, K., 1985: Neonatal hyperbilirubinemia: management and prognosis. Nihon Rinsho. Japanese Journal of Clinical Medicine 43(8): 1734-1740

Mease, P.J.; Ginzler, E.M.; Gluck, O.S.; Schiff, M.; Goldman, A.; Greenwald, M.; Cohen, S.; Egan, R.; Quarles, B.J.; Schwartz, K.E., 2005: Effects of prasterone on bone mineral density in women with systemic lupus erythematosus receiving chronic glucocorticoid therapy. Objective. To assess the effects of treatment with prasterone (dehydroepiandrosterone) on bone mineral density (BMD) in female patients with mild to moderate systemic lupus erythematosus (SLE) receiving chronic treatment with glucocorticoids.Metho...

Jinno, A.; Maruyama, Y.; Ishizuka, M.; Kazusaka, A.; Nakamura, A.; Fujita, S., 2005: Induction of cytochrome P450-1A by the equine estrogen equilenin, a new endogenous aryl hydrocarbon receptor ligand. Equilenin is one of 10 kinds of estrogens that are found in pregnant mares' urine. It has been used extensively for estrogen replacement therapy in postmenopausal women. Typical inducers of the cytochrome P4501A1 (CYP1A1), such as TCDD, benzo...

Sakai K.; Sastre J.P.; Salvert D.; Touret M.; Tohyama M.; Jouvet M., 1979: Tegmento reticular projections with special reference to the muscular atonia during paradoxical sleep in the cat a horseradish peroxidase study. Using the retrograde tracer technique with horseradish peroxidase (HRP), attempts were made to determine the cells of origin and the descending pathway of the tegmentoreticular projections to give an anatomical substrate for the physiological phen...

Wang, J.; Wang, R.; Yang, X-Ye., 2013: Efficacy of an Arthrobotrys oligospora N mutant in nematode-trapping larvae after passage through the digestive tract of sheep. Veterinary Microbiology 161(3-4): 359-361

Gordon, J., 2003: Fostering students' personal and professional development in medicine: a new framework for PPD. Altruism, accountability, duty, integrity, respect for others and lifelong learning are qualities that have been identified as central to medical professionalism. However, we do not have a systematically developed understanding of what is needed t...

Bacq, Z.M.; Fischer, P., 1950: On the existence of adrenaline-quinone. Archives Internationales de Physiologie 57(3): 271-276