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Interleukin-1 beta stimulates interleukin-6 production in placental villous core mesenchymal cells

, : Interleukin-1 beta stimulates interleukin-6 production in placental villous core mesenchymal cells. Endocrinology 134(1): 457-460

Interleukin-6 (IL-6) is a pleiotropic mediator of immune function and a growth factor for a variety of hematopoietic cell types. Because IL-1 beta is known to induce IL-6 production in nonplacental mesenchymal cells and is locally produced by maternal decidua, this study was designed to determine whether IL-1 beta could regulate IL-6 production by second trimester placental villous core mesenchymal cells (VCMC) in vitro. VCMC were prepared for culture by enzymatic digestion of placentas (14-20 weeks gestation; n = 7). Immunohistochemistry performed on the confluent cells demonstrated that more than 95% of the cells had a fibroblast-like morphology and were vimentin positive, less than 5% were leukocyte common antigen (CA-45) positive, and no trophoblast contamination was demonstrated by the lack of cytokeratin staining. In dose-response experiments, a specific dose-response induction of IL-6 mRNA expression and IL-6-immunoreactive protein production by IL-1 beta was demonstrated; this was first seen at 100 pg/ml IL-1 beta [455 +/- 191 ng/ml (+/- SEM); controls, 42 +/- 16 ng/ml; P < 0.05]. In time-course studies, the addition of 10 ng/ml IL-1 beta significantly increased IL-6 production rates; this was first seen at 8 h of culture and increased in a linear fashion up to 48 h. At 48 h of culture, IL-6 levels were 17 times higher in treated VCMC (861 +/- 179 ng/ml) compared to those in nontreated VCMC (51 +/- 14 ng/ml). In summary, IL-1 beta stimulates VCMC IL-6 production in a specific dose- and time-dependent manner. From these results, we conclude that VCMC are an important source of IL-6 in second trimester placenta and that production of placental IL-6 be may regulated by decidual IL-1 beta.


PMID: 8275959

DOI: 10.1210/endo.134.1.8275959

Other references

Brauer, J.C., 1951: Preventive service and the general practitioner. (What is preventive dentistry?). Journal of the American College of Dentists 18(3): 146-149

Pang, D.; Wilberger, J.E., 1980: Upward migration of peritoneal tubing. A case of upward migration of the peritoneal tubing of a subdural peritoneal shunt into a large craniotomy skin flap in a human infant is presented. The probable mechanisms are discussed. Suggestions are made for how to avoid the occurrence of thi...

Macan, J.; Babić, Željka.; Samardžić, T., 2017: Skin health and protection at work in Croatian beautician apprentices. Contact Dermatitis 76(3): 178-179

Krapivskiy Y.I.; Sergeyev L.Y.; Kovalev A.A.; Artamonov S.V., 1981: Instruments for neutron-neutron logging with automatically processed measurement results. Geofizicheskaya Apparatura 72): 124-129

Whitby, P.W.; Stull, T.L., 1998: PCR as a diagnostic tool for the rapid detection of Burkholderia cepacia in the sputum of patients with cystic fibrosis. Pediatric Research 43(4 PART 2): 336A, April

Jovani, M.; Baticci, F.; Bonifacio, C.; Omodei, P.Dario.; Malesci, A., 2014: Abdominal cocoon or idiopathic encapsulating peritoneal sclerosis: magnetic resonance imaging. Digestive and Liver Disease 46(2): 192-193

Asciore, L.; Pescatori, L.C.; Pescatori, M., 2016: Semi-closed bilateral partial miotomy of the puborectalis for anismus: a pilot study: Partial miotomy of the puborectalis for anismus. Anismus or non relaxing puborectalis muscle (PRM) may cause obstructed defecation (OD). Reported surgical treatment is partial miotomy, followed by sepsis, bleeding, and incontinence. The aim of the present study was to investigate on the feasibil...

Fischbach, F.; Lehmann, T.N.; Ricke, J.; Bruhn, H., 2003: Vascular compression in glossopharyngeal neuralgia: demonstration by high-resolution MRI at 3 tesla. We report a case of glossopharyngeal neuralgia with vascular compression. High-resolution MRI at 3 tesla demonstrated the posterior inferior cerebellar artery to be closely related to the rootlets of the left glossopharyngeal nerve in a patient wh...

Cody M.L.; Diamond J.M., 1975: Ecology and evolution of communities. The 18 papers presented and discussed at the symposium in memory of Robert MacArthur are included. Three broad classes of ecological problems are considered, each in its own section. The first section discusses diversity and relative abundance of...

Spitz, F., 1974: Demographic du campagnol des champs Microtus arvalis en Vendee. Annales Zool Ecol anim, 62: 259-312