geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Interstrand disulfide cross-linking of internal sugar residues in duplex RNA


, : Interstrand disulfide cross-linking of internal sugar residues in duplex RNA. Bioorganic & Medicinal Chemistry 8(1): 269-273

Disulfide cross-linking is being used increasingly more to study the structure and dynamics of nucleic acids. We have previously developed a procedure for the formation of disulfide cross-links through the sugar-phosphate backbone of nucleic acids. Here we report the preparation and characterization of an RNA duplex containing a disulfide interstrand cross-link. A self-complementary oligoribonucleotide duplex containing an interstrand cross-link was prepared from the corresponding 2'-amino modified oligomer. Selective modification of the 2'-amino group with an aliphatic isocyanate, containing a protected disulfide, gave the corresponding 2'-urea derivative in excellent yield. An RNA duplex containing an intrahelical, interstrand disulfide cross-link was subsequently prepared by a thiol disulfide exchange reaction in nearly quantitative yield as judged by denaturing polyacrylamide gel electrophoresis (DPAGE). The cross-linked RNA was further characterized by enzymatic digestion and the Structure of the cross-link lesion was verified by comparison to an authentic sample, prepared by chemical synthesis. The effect of the chemical modifications on duplex stability was determined by UV thermal denaturation experiments. The intrahelical cross-link stabilized the duplex considerably: the disulfide cross-linked oligomer had a melting temperature that was ca. 40 degrees C higher than that of the noncross-linked oligomer.

US$29.90

PMID: 10968286


Other references

Hill, B.T.; Copelan, E.A., 2010: Acute myeloid leukemia: when to transplant in first complete remission. Allogeneic hematopoietic stem cell transplantation (HSCT) is commonly used to treat acute myeloid leukemia (AML) because it is potentially curative when other therapies have a low likelihood of success. Although most patients with newly diagnosed...

Nerantzis, C.E.; Gavrielatos, G.; Kalogrias, N.; Pappas, L.K.; Letsas, K.P.; Karakoukis, N.G.; Koutsaftis, P.N., 2008: Histological recognition and classification of the atrioventricular node artery variants: a new approach. There is a controversy in the literature concerning the origin, course, and distribution of the atrioventricular (AV) node artery. Postmortem coronary angiography, dissection, and microscopic examination were performed in 100 human hearts specimen...

Delwart, E., 2013: A roadmap to the human virome. Plos Pathogens 9(2): E1003146-E1003146

Conrad, J.C.; Osborn, J.B.; Conrad, K.J.; Jetzer, T.C., 1990: Peripheral nerve dysfunction in practicing dental hygienists. Hand-intensive repetitive procedures such as those performed by dental hygienists can lead to cumulative trauma disorders of the upper extremity. One such disorder is median nerve dysfunction leading to carpal tunnel syndrome (CTS). Vibrometry has...

Bui, T.D.; Kadzakumanja, O.; Munthali, C., 2015: Mobilizing for the Lilongwe Diabetes Peer Support Programme in Malawi. Diabetes has become a significant cause of morbidity and mortality in Malawi but there are shortages of drug supply and healthcare providers to support quality care and treatment. Diabetes self-management support is necessary to improve patient ou...

Hobbelink, H., 1988: Patenting of life forms: how to react. A brief account is given of the development of patenting systems for plant varieties and, to a lesser extent, animal breeds. Also discussed are recent EC proposals that would allow the patenting of all living organisms, processes and techniques, a...

Murawski, C.D.; Kennedy, J.G., 2011: Anteromedial impingement in the ankle joint: outcomes following arthroscopy. Arthroscopic debridement is a well-accepted method of removing osseous and/or soft tissue impingement from the ankle joint. To the best of the authors' knowledge, this is the first case series reporting the outcomes following arthroscopic res...

Gong, J.; Xu, L.; Yang, Y.; Chen, D-Yun.; Ran, Y., 2011: Sequential ASE extraction of alkylphenols from sediments: Occurrence and environmental implications. The occurrence of alkylphenols (APs) including nonylphenol (NP) and octylphenol (OP) in the riverine sediments from the Pearl River Delta (PRD), South China was investigated and compared by Soxhlet extraction (S-APs) with dichloromethane and by se...

Albanese, A.; Stanhope, R.; Jones, J.; Matthews, D.R., 1996: Short-term growth hormone effect on endogenous growth hormone secretion in children with growth delay in childhood. Hormone Research (Basel) 46(SUPPL 2): 11

Ohno, K.; Minami, T.; Matsui, Y.; Magara, Y., 2007: Effects of chlorine on organophosphorus pesticides adsorbed on activated carbon: desorption and oxon formation. We investigated effects of chlorination on four organophosphorus pesticides (diazinon, isoxathion, malathion, and tolclofos-methyl) adsorbed on powdered activated carbon (PAC). Following adsorption of each pesticide on 10mg/L of PAC in water, chl...