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Isolation and promoter analysis of two genes encoding different endo-beta-1,4-glucanases in the non-climacteric strawberry

, : Isolation and promoter analysis of two genes encoding different endo-beta-1,4-glucanases in the non-climacteric strawberry. Journal of Experimental Botany 54(381): 271-277

Two endo-beta-1,4-glucanase (EGase; EC genes, highly expressed during ripening of the non-climacteric strawberries (Fragariaxananassa Duch. cv. Chandler), were isolated. Serial promoter deletions of both genes (i.e. FaEG1 and FaEG3) fused to GUS were transiently assayed in strawberry fruits by using a technique recently developed in this laboratory. Although differences were observed with the short fragments, GUS activity became comparable with the largest fragments of both promoters. The apparently similar strength of the two largest promoter fragments was in contrast with previous results of Northern analyses which demonstrated different transcripts amounts for the two genes. The inclusion of the 3' flanking region of both genes in the transient assays showed that, in the case of FaEG3, the 3' region had a down-regulating effect on the expression of GUS, and this might account for the lower amount of FaEG3 mRNA usually observed in ripe fruits compared to that of FaEG1. Downstream instability elements might be involved in such down-regulation.


PMID: 12493854

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Petty, E.M.; Gibson, L.H.; Fountain, J.W.; Bolognia, J.L.; Yang-Feng, T.L.; Housman, D.E.; Bale, A.E., 1993: Molecular definition of a chromosome 9p21 germ-line deletion in a woman with multiple melanomas and a plexiform neurofibroma: implications for 9p tumor-suppressor gene(s). Cutaneous malignant melanoma (CMM) is often familial, but the mode of inheritance and the chromosomal location of a melanoma susceptibility locus are controversial. Identification of a 34-year-old woman with eight primary malignant melanomas, mult...

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Kharkyanen, V.N.; Petrov, E.G.; Ukrainskii, I.I., 1978: Donor acceptor model of electron transfer through proteins. The interaction of proteins with a polar solvent leads to the formation of electron donor groups in the proteins. The highest occupied level of these groups in situated near the bottom of the conduction energy band of protein. The transfer of an e...

Damle, N.K.; Mohagheghpour, N.; Engleman, E.G., 1984: Activation of antigen-specific suppressor T lymphocytes in man involves dual recognition of self class I MHC molecules and Leu-4/T3-associated structures on the surface of inducer T lymphocytes. We showed previously that fresh Leu-2+ T cells respond to autologous antigen-primed Leu-3+ T cells by proliferation and differentiation into suppressor T cells (Ts) that specifically inhibit the response of fresh Leu-3+ cells to the original primi...

Oehmichen, M.; Schlote, W.; Mallach, H.J., 1983: Brain changes in parathion poisoning: observations in 42 cases. A total of 42 cases were examined neuropathologically to determine possible toxic changes occurring in the brain after parathion intoxication. Sporadic anoxic alterations were observed in 7% of 41 cases in which the cause of death was acute intoxi...

Kane T.C.; Poulson T.L., 1973: Field experiments in simple cave communities predation strategies of 2 co occurring carabid beetles. American Zoologist 13(4): 1328

Sarma, D.S.; Zubroff, J.; Rajalakshmi, S., 1977: Repair in vivo of liver deoxyribonucleic acid damaged by hycanthone and related compounds. Molecular Pharmacology 13(4): 719-725

Shaw, J.; Kirshenbaum, L.A., 2006: HAX-1 represses postmitochondrial caspase-9 activation and cell death during hypoxia-reoxygenation. Based on findings of experimental models of diabetes mellitus (DM) showing increased expression of vascular cyclooxygenase-2 (COX-2), we hypothesized that in patients with DM changes in COX-2-dependent prostaglandin synthesis affect vasomotor resp...

Wachs, E., 1957: Treatment of lipomatosis dolorosa (Dercum). Zentralblatt für Chirurgie 82(42): 1787-1792