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Leukocyte infiltration and intercellular adhesion molecule-1-mediated cell interactions in immunoglobulin A nephropathy


, : Leukocyte infiltration and intercellular adhesion molecule-1-mediated cell interactions in immunoglobulin A nephropathy. Archives of Pathology & Laboratory Medicine 122(9): 817-822

Mononuclear leukocytes have a role in immunoglobulin (Ig) A nephropathy. Renal leukocyte recruitment is mediated by adhesive interactions between leukocytes and their ligands on renal cells. We assessed interstitial and glomerular leukocytes using an avidin-biotin-peroxidase method with monoclonal antibodies (MAbs) against leukocytes (CD45), beta2-integrin (CD18), macrophages (CD14), T cells (CD3) and T-cell subsets (CD4, CD8), intercellular adhesion molecule-1 (ICAM-1) (CD54), and HLA class II antigens. We analyzed their relation with the abnormal expression of ICAM-1 on proximal tubular epithelium in sequential renal sections from 57 patients with IgA nephropathy stratified by degrees of interstitial cellular infiltration as seen by light microscopy. In IgA nephropathy without infiltration (n = 15) and with interstitial cellular infiltration of 1+ by light microscopy (n = 11), ICAM-1 expression on vascular endothelium was unchanged with respect to that observed in the normal kidney; the proximal tubular epithelium was negative for this stain. Moreover, in IgA nephropathy with interstitial cellular infiltration greater than or equal to 2+ by light microscopy (n = 31), ICAM-1+ stain was seen on proximal tubular epithelium. The value of ICAM-1+ tubule staining was significantly (P = .0004) higher in the biopsies with a higher degree of interstitial cellular infiltration seen by light microscopy. The tubular ICAM-1+ stain was significantly associated with the number of interstitial leukocytes identified by the MAbs tested and correlated (P < .05) with CD45+ (r = 0.59), CD14+ (r = 0.54), and CD3+ (r = 0.51) interstitial leukocytes in IgA nephropathy with interstitial cellular infiltration by light microscopy. Interstitial ICAM-1+ cells and interstitial CD18+ leukocytes were correlated (r = 0.85, P < .001). Correlation was found between the value of ICAM-1+ tubule staining and the number of CD45+ (r = 0.87, P < .01), CD14+ (0.58, P < .05), and CD8+ (r = 0.68, P < .05) glomerular leukocytes. Our results suggest that tubular and interstitial ICAM-1+ cells may participate in adhesive interactions with interstitial leukocytes. Epithelial tubular cells expressing ICAM-1 may be a marker of tubulointerstitial disturbance upregulated by interstitial and glomerular infiltration of macrophages and T cells in IgA nephropathy.

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PMID: 9740142


Other references

Amyot E.; Hurnik J.F., 1986: Manifestation of estrus in dairy cows. Canadian Journal of Animal Science 66(1): 317

Olveda, D.U.; Li, Y.; Olveda, R.M.; Lam, A.K.; McManus, D.P.; Chau, T.N.P.; Harn, D.A.; Williams, G.M.; Gray, D.J.; Ross, A.G.P., 2014: Bilharzia in the Philippines: past, present, and future. Schistosomiasis japonica has a long history in the Philippines. In 1975, 24 endemic provinces were identified in the northern, central, and southern islands of the Philippines. More than five million people were at risk, with approximately one mil...

Bruun, H., 1978: Summary of the east Lapland observations from the 1978 summer.. Baptria (Helsinki): 34: 81-83

Ferranti, P.; Melck, D.; Caira, S.; Picariello, G.; Chianese, L.; Addeo, F., 2002: Evaluation of functional peptides in milk and cheese products using mass spectrometry. A review, dealing with the identification of the casein proteolysis mechanism in cheese, the biological activity of phosphopeptides and their use as cheese quality markers, the identification of inhibitory ACE peptides and bioactive peptides in ch...

Biino, L., 1971: Floral and honey-dew honey. Rassegna chimica: 23 (3) 99-103

Lippmann, M.; Ito, K.; Hwang, J-Shiang.; Maciejczyk, P.; Chen, L-Chi., 2006: Cardiovascular effects of nickel in ambient air. BACKGROUND: Fine particulate matter (FPM) in ambient air causes premature mortality due to cardiac disease in susceptible populations.OBJECTIVE: Our objective in this study was to determine the most influential FPM components.METHODS: A mouse mod...

Mangelschots K.; Coucke P.; Speleman F.; Bossuyt P.; Van Oostveldt P.; Van Der Auwera B.; Carrit B.; Willems P.J., 1991: Localization of the fucosidase pseudogene fuca1p to 2q31 q32. Cytogenetics & Cell Genetics 58(1-4): 1870

Lin, Y-Hsun.; Ryan, C.S.; Davies, J.K., 2011: Neisserial Correia repeat-enclosed elements do not influence the transcription of pil genes in Neisseria gonorrhoeae and Neisseria meningitidis. Two human-specific neisserial pathogens, Neisseria gonorrhoeae and Neisseria meningitidis, require the expression of type IV pili (tfp) for initial attachment to the host during infection. However, the mechanisms controlling the assembly and funct...

Ali Zeilani, R.S.; Al-Nawafleh, A.H.; Evans, C., 2012: Looking back at the doctorate: a qualitative study of Jordanian graduates from PhD programs in the UK. Many countries develop their nursing capacity by sending nurses overseas to gain their doctorates. There is a lack of research on their subsequent experience and career development. Focusing on the Jordanian and UK contexts, this qualitative study...

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