geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Macrophage targeting with technetium-99m labelled J001 acylated poly-galactoside for scintigraphy of inflammation: optimization and assessment of imaging specificity in experimental arthritis


, : Macrophage targeting with technetium-99m labelled J001 acylated poly-galactoside for scintigraphy of inflammation: optimization and assessment of imaging specificity in experimental arthritis. European Journal of Nuclear Medicine 23(1): 61-68

J001, an acylated poly-(1,3)-galactoside purified from the membrane of Klebsiella pneumoniae, associates selectively with macrophages via the binding to CD11b and CD14 molecules. Inflammatory foci known to recruit macrophages could thus be imaged with technetium-99m labelled J001. This study aims to define the optimal scintigraphic protocol for 99mTc-J001 imaging and to evaluate the specificity of J001 scans. A dose range study was conducted in rabbits with immunological arthritis using six different specific activities ranging from 370 to 11840 MBq·mg-1 while the intravenously injected activity was constant (37 MBq) Radiochemical purity for each preparation was documented together with the in vivo stability of the 99mTc-J001 complex using exclusion-diffusion radioHPLC of serum collected 1 h after radiopharmaceutical administration. Scintigraphic images were recorded at 2, 3 and 4h and analysed using indexes calculated from regions of interest. Specificity of the macrophage imaging was assessed by comparison with scans obtained after administration of 99mTcO4(- )or 99mTc-albumin nanocolloids. A protocol of plasma transfusion was also used to inject 99mTc-J001 after complete removal of radioactive colloids likely to be generated during the labelling. For the higher specific activities (5920 and 11840 MBq.mg-1), radiochemical purity degradation and in vitro 99mTc transchelation were noted. To prevent transchelation and 99mTc bond hydrolysis likely to impair imaging specificity, 1480 MBq.mg-1 corresponding to 25microg injected J001 was found to be the optimal usable specific activity. Results obtained with the various tracers support the hypothesis that macrophage targeting is the main factor involved in the J001 imaging of arthritis.

US$29.90

PMID: 8586104


Other references

Yadav, U.C.S.; Ramana, K.V.; Srivastava, S.K., 2015: Aldose reductase regulates acrolein-induced cytotoxicity in human small airway epithelial cells. Aldose reductase (AR), a glucose-metabolizing enzyme, reduces lipid aldehydes and their glutathione conjugates with more than 1000-fold efficiency (<italic>K</italic> <inf loc="post">m</inf> aldehydes 5 30<hsp...

Iwatani, S.; Nagashima, H.; Reddy, R.; Shiota, S.; Graham, D.Y.; Yamaoka, Y., 2015: Identification of the genes that contribute to lactate utilization in Helicobacter pylori. Helicobacter pylori are Gram-negative, spiral-shaped microaerophilic bacteria etiologically related to gastric cancer. Lactate utilization has been implicated although no corresponding genes have been identified in the H. pylori genome. Here, we r...

Lacourt J., 1974: Rare or new tenthredinidae for france. Entomologiste (Paris) 30(3): 116-120

Zhao, Z-Gang.; Wang, Y-Ke., 2015: Selection by pollinators on floral traits in generalized Trollius ranunculoides (Ranunculaceae) along altitudinal gradients. Abundance and visitation of pollinator assemblages tend to decrease with altitude, leading to an increase in pollen limitation. Thus increased competition for pollinators may generate stronger selection on attractive traits of flowers at high elev...

Anonymous, 1991: An antifreeze glycopeptide gene from the antarctic cod Notothenia coriiceps neglecta encodes a polyprotein of high peptide copy number. Proceedings of the National Academy of Sciences of the United States of America 88(7): 2966-2966

Runge, V.M.; Armstrong, M.R.; Barr, R.G.; Berger, B.L.; Czervionke, L.F.; Gonzalez, C.F.; Halford, H.H.; Kanal, E.; Kuhn, M.J.; Levin, J.M.; Low, R.N.; Tanenbaum, L.N.; Wang, A.M.; Wong, W.; Yuh, W.T.; Zoarski, G.H., 2001: A clinical comparison of the safety and efficacy of MultiHance (gadobenate dimeglumine) and Omniscan (Gadodiamide) in magnetic resonance imaging in patients with central nervous system pathology. RATIONALE AND OBJECTIVES. The safety and diagnostic efficacy of MultiHance (gadobenate dimeglumine) in the central nervous system (CNS) were evaluated in a double-blind, multicenter, phase III clinical trial. METHODS. Two hundred five patients hig...

Cooley; Aronson; Fisk; Canfield, 1995: SmB6: Kondo insulator or exotic metal?. Physical Review Letters 74(9): 1629-1632

Leslie, G., 1879: The Dentition of Hypsiprymnus (Bettongia) Penicillatus, Gray. Journal of Anatomy and Physiology 13(Pt 4): 546-548

Oger, Jël., 2007: Immunosuppression: promises and failures. The author participated very early in the use of immunosuppressors in the treatment of multiple sclerosis. He reviews evidence which support their use. IV Methylprednisolone, azathioprine and mitoxantrone are supported in their use by evidence of...

Desai A.; Eymontt M.; Alavi A.; Schaffer B.; Dalinka M.K., 1980: Technetium 99m labeled methylene di phosphonate uptake in nonosseous lesions. Radiology 135(1): 181-184