geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Mitogen-activated protein kinase plays an important role in hemolysate-induced contraction in rabbit basilar artery


, : Mitogen-activated protein kinase plays an important role in hemolysate-induced contraction in rabbit basilar artery. Acta Neurochirurgica. Supplement 76(): 217-221

Mitogen-activated protein kinase (MAPK) is an important signaling factor in the vascular proliferation and contraction, the two features of cerebral vasospasm following subarachnoid hemorrhage. We studied the possible involvement of MAPK in hemolysate-induced signal transduction and contraction in rabbit basilar artery. Isometric tension was used to record the contractile response of rabbit basilar artery to hemolysate. Western blots using antibodies for MAPK were conducted. 1) Hemolysate produced a concentration-dependent contraction of rabbit basilar artery. Pre-incubation of arteries with MAPK kinase inhibitor PD-98059 markedly reduced the contraction induced by hemolysate. PD-98059 also relaxed, in a concentration-dependent fashion, the sustained contraction induced by hemolysate (10%). 2) Hemolysate produced a time-dependent elevation of MAPK immunoreactivity in Western blot in rabbit basilar artery. MAPK was enhanced 3 min after hemolysate exposure and the effect reached maximum at 5 min. The immunoreactivity of MAPK decayed slowly with time, but the level of MAPK was still higher than the basal level even at two hours after exposure to hemolysate. 3) Pre-incubation of arteries with MAPK kinase inhibitor PD-98059 abolished the effect of hemolysate on MAPK immunoreactivity. Hemolysate produced contraction of rabbit basilar artery possibly by activation of MAPK. MAPK inhibitors may be useful in the treatment of cerebral vasospasm.

US$29.90

PMID: 11450011


Other references

King, C.D.; Rios, G.R.; Green, M.D.; Tephly, T.R., 2000: UDP-glucuronosyltransferases. Current Drug Metabolism 1(2): 143-161, September

Oppert, J.M.; Basdevant, A.; Guy Grand, B., 1993: Glucose transporters and obesity. The 2 classes of glucose transporters, the cotransporter glucose-Na+ and that family of proteins responsible for easy diffusion of glucose, are described. The system of transport of glucose by diffusion and its regulation in the course of obesity...

Evans G.S.; Potten C.S., 1988: The distribution of endocrine cells along the mouse intestine a quantitative immunocytochemical study. The topographical distribution of endocrine cells in the crypt and villus epithelium along the length of the mouse intestine was studied. Argyrophil reactivity using the Grimelius stain was used to estimate the total endocrine population of the in...

Kimura, M.; Minamiguchi, H.; Sahara, S.; Yamada, K.; Nakai, M.; Tanihata, H.; Kishi, K.; Terada, M.; Shioyama, Y.; Sato, M., 2003: Diagnosis of deep vein thrombosis using multi-detector helical CT. To evaluate the usefulness of multi-detector helical CT (MDHCT) with contrast medium in the diagnosis of deep vein thrombosis (DVT). The bilateral veins of the dorsal pedis in 45 patients (12 men, 33 women; average age, 64 years) under clinical su...

Kitano, T.; Kawasaki, Y.; Sakamoto, K., 1992: Instrumental evaluations of noncationic materials derived from amino acids as hair conditioning agents. Skin Pharmacology 5(4): 242-243

Daly, J.M.; Jensen, S.G., 1961: Evidence for functional cytochrome oxidase in tissues infected by obligate parasites. Ascorbic acid and polyphenol oxidases have been suggested previously to be of importance in infected plant tissues, especially obligate parasites. The evidence has been obtained largely by use of the homog-enate technique. Since at least 40% of th...

Wilkinson, R.D.; Tavaré, S., 2009: Estimating primate divergence times by using conditioned birth-and-death processes. The fossil record provides a lower bound on the primate divergence time of 54.8 million years ago, but does not provide an explicit estimate for the divergence time itself. We show how the pattern of diversification through the Cenozoic can be com...

Mukherjee, J.J.; Dekker, E.E., 1987: Purification, properties, and N-terminal amino acid sequence of homogeneous Escherichia coli 2-amino-3-ketobutyrate CoA ligase, a pyridoxal phosphate-dependent enzyme. Starting with 100 g (wet weight) of a mutant of Escherichia coli K-12 forced to grow on L-threonine as sole carbon source, we developed a 6-step procedure that provides 30-40 mg of homogeneous 2-amino-3-ketobutyrate CoA ligase (also called aminoac...

Agorastos T.; Economou Amilli A.; Papaloukas A., 1984: Scanning electron microscopy of the stratum corneum of human newborn epidermis at term. Acta Anatomica 120(1-2): 3-4

Kraeva L.N.; Pozdnev V.F.; Kokryakov V.N.; Usova A.A., 1991: Synthetic substrates in measurement of serine proteinase esterase activity. The authors suggest a highly sensitive rapid and simple method for measuring esterase activities of bovine pancreatic chemotrypsin, human neutrophilic cathepsin D, and elastase, and of human blood serum chymotrypsin-like esterase and elastase-like...