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Multiple inhibitory mechanisms of wild-type p16 gene transfer into human bladder cells by retroviral vector


, : Multiple inhibitory mechanisms of wild-type p16 gene transfer into human bladder cells by retroviral vector. Zhonghua Wai Ke Za Zhi 37(8): 456-458

To investigate the inhibitory mechanisms of wild-type p16 gene transfer into human bladder cancer cells with or without endogenous p16 gene expression. p16 recombinant retrovirus vector was constructed and transfected in human bladder cancer cell EJ and 253J. Northern blot analysis and immunocytochemistry were used to detect the expression of exogenous p16 gene. Flow cytometry analysis was used to detect tumor cell cycle. Electronic microscope and in situ labelling apoptotic DNA fragment detection (TUNEL) was used to detect tumor cell apoptosis after gene transfection. Tumorigenicity was observed in nude mice. The growth rate of the transfected EJ cells was significantly retarded, the majority of transfected EJ cells were arrested on G(0) + G(1) phases of cell cycle, tumorigenicity of the transfected cells was reduced on nude mice. H-ras gene expression in transfected EJ cells reduced as compared with non-transfection. Apoptotic cells were observed in transgenic 253J cells. Malignant proliferation of bladder cancer cells can be inhibited by exogenous p16 gene transfection via different molecular pathway.

US$29.90

PMID: 11829887


Other references

Blagosklonnaya Y.V.; Mamedov R.; Kozlov V.V.; Emanuel' V.L.; Kudryashova M.I., 1982: Effect of trental on some renal function parameters in diabetes mellitus patients. Effect of trental on some renal function parameters was studied in 11 patients with diabetes mellitus; 7 of these patients had clinical signs of diabetic nephropathy. Trental was administered by i.v. drip in a dose of 300 mg daily during 3 wk. Mar...

Richardson, M.W.; Grewal, S.S., 2007: Use of imatinib mesylate in a 15-month old with Philadelphia chromosome positive (Ph+) chronic myelogenous leukemia. Pediatric Blood & Cancer 50(1): 186-186

Kass, D.I., 1998: Economies of scope and home healthcare. Health Services Research 33(4 Pt 1): 947-951

Bay, I.; Kardel, K.M.; Skougaard, M.R., 1973: Plaque-removing ability of toothbrushes. A quantitative evaluation. Royal Society of Health Journal 93(4): 218-221

Kwon, S.Young.; Park, S.Hoon.; Yeon, J.Eun.; Jeong, S.Hyang.; Kwon, O.Sang.; Lee, J.Woo.; Kim, H.Soo.; Seo, Y.Seok.; Kim, Y.Seok.; Sohn, J.Hyun.; Yim, H.Joon.; Choi, J.Young.; Lee, M.Seok.; Kweon, Y.Oh.; Cheong, J.Youn.; Kim, H.Cheoul.; Lee, H.Ju.; Baik, S.Koo.; An, H.; Byun, K.Soo., 2014: Clinical characteristics and outcomes of acute hepatitis a in Korea: a nationwide multicenter study. The aim of this study was to investigate the clinical characteristics of acute hepatitis A during a recent outbreak in Korea. Data of patients diagnosed with acute hepatitis A from 2007 to 2009 were collected from 21 tertiary hospitals retrospecti...

Fader, L.D.; Carreira, E.M., 2004: Stereochemically rich pentaketides from bis(isoxazolines): a general strategy for efficient polyketide synthesis. [reaction: see text] A strategy based on diastereoselective dipolar cycloaddition reaction of nitrile oxides and allylic alcoholates has been applied to the synthesis of bis(isoxazolines) that are precursors to polyketide fragments. These intermed...

Angrisani, R.Mariangela.Giaffredo.; Azevedo, M.Frasson.de.; Carvallo, R.Mota.Mamede.; Diniz, E.Maria.de.Albuquerque.; Ferraro, A.Archanjo.; Guinsburg, R.; Matas, C.Gentile., 2014: Electrophysiological characterization of hearing in small for gestational age premature infants. To characterize the Auditory Brainstem Response (ABR) of small for gestational age preterm newborns and to compare the findings to those of appropriate for gestational age premature newborns in order to verify whether the small for gestational age...

Genova, M., 1974: Preventive measures in the diagnosis of surgical congenital heart diseases. Khirurgiia 27(1): 37-41

Rassow, J.; Meinecke, M., 2013: Helicobacter pylori VacA: a new perspective on an invasive chloride channel. The vacuolating cytotoxin VacA, a polypeptide of about 88 kDa, is one of the major virulence factors of Helicobacter pylori. VacA essentially acts as an invasive chloride channel targeting mitochondria. The results of recent studies open a new per...

Jhun, B.Sook.; Lee, J.Yeon.; Oh, Y.Taek.; Lee, J.Hie.; Choe, W.; Baik, H.Hwan.; Kim, S.Soo.; Yoon, K-Sik.; Ha, J.; Kang, I., 2006: Inhibition of AMP-activated protein kinase suppresses IL-2 expression through down-regulation of NF-AT and AP-1 activation in Jurkat T cells. AMP-activated protein kinase (AMPK) is a key regulator of energy homeostasis and its activation during T cell receptor stimulation has recently been reported. In this study, we examined the role of AMPK in interleukin (IL)-2 production in T cells...