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Pyruvate formate-lyase-activating enzyme: strictly anaerobic isolation yields active enzyme containing a [3Fe-4S](+) cluster

, : Pyruvate formate-lyase-activating enzyme: strictly anaerobic isolation yields active enzyme containing a [3Fe-4S](+) cluster. Biochemical and Biophysical Research Communications 269(2): 451-456

Pyruvate formate-lyase-activating enzyme (PFL-AE) from Escherichia coli (E. coli) catalyzes the stereospecific abstraction of a hydrogen atom from Gly734 of pyruvate formate-lyase (PFL) in a reaction that is strictly dependent on the cosubstrate S-adenosyl-l-methionine (AdoMet). Although PFL-AE is an iron-dependent enzyme, isolation of the enzyme with its metal center intact has proven difficult due to the oxygen sensitivity and lability of the metal center. We report here the first isolation of PFL-AE under nondenaturing, strictly anaerobic conditions. Iron and sulfide analysis as well as UV-visible, EPR, and resonance Raman data support the presence of a [3Fe-4S](+) cluster in the purified enzyme. The isolated native enzyme, but not apo-enzyme, exhibits a high specific activity (31 U/mg) in the absence of added iron, indicating that the native cluster is necessary and sufficient for enzymatic activity.


PMID: 10708574

DOI: 10.1006/bbrc.2000.2313

Other references

Longarato Jacometta, F.; Sidari, A., 1965: Observation on "1st admittance" cases of pulmonary tuberculosis in the Ospedale Sanitoriale INPS di Rovigo. (Clinico-statistical investigation related to the last 2-year period). Il Friuli Medico 20(4): 501-513

Wilcox J.N.; Roberts J.L., 1985: Estrogen decreases rat hypothalamic proopiomelanocortin messenger rna levels. Ovariectomy and estrogen (E) and E plus progesterone treatment has previously been shown to alter both hypothalamic content and portal plasma levels of .beta.-endorphin. To determine if these changes were accompanied by changes in .beta.-endorphin...

Hwang; Wu, 1994: Quasi-two-dimensional domain structures of magnetic particles in a static field. Physical Review. E, Statistical Physics, Plasmas, Fluids, and Related Interdisciplinary Topics 49(4): 3102-3108

Rejman, J.; Conese, M.; Hoekstra, D., 2006: Gene transfer by means of lipo- and polyplexes: role of clathrin and caveolae-mediated endocytosis. In this paper we address the contribution of different endocytic pathways to the intracellular uptake and processing of differently sized latex particles and of plasmid DNA complexes by means of fluorescence microscopy and FACS analysis. By using...

Spiegelman, A.R.; Quigley, T.J., 1952: Isonicotinic acid hydrazide in the tuberculous diabetic. Quarterly Bulletin of Sea View Hospital. New York. Sea View Hospital, Staten Island. Clinical Society 13(4): 203-204

Gatz C., 1988: The tn 10 encoded tet repressor operator interaction is functional in plant cells. Journal of Cellular Biochemistry Supplement (12 PART D): 135

Monteagudo Sánchez, B.; Ginarte Val, M.; Vega, A.; Used Aznar, M.M., 2008: Oral papillomatosis. Revista Clinica Espanola 208(6): 305-306

Stewart, J.; Schulz-Baldes, M., 1976: Long term lead accumulation in abalone haliotis spp fed on lead treated brown algae egregia laevigata. The amount of Pb accumulated in the body of a grazing mollusk by transfer from its algal food in laboratory experiments was assessed, these results were compared with the amounts found in naturally occurring mollusks and seaweed. Near La Jolla, Ca...

Epifanova, N.V.; Novikova, N.A.; Efimov, E.I.; Parfenova, O.V.; Lukovnikova, L.B.; Fomina, S.G., 2013: Molecular-genetic characteristic of astroviruses circulating in Nizhny Novgorod. Genotyping ofastroviruses isolated from children with acute enteric infection (AEI) on the territory of Nizhny Novgorod in 2006 - 2011. Samples of feces from children hospitalized in intestine infection department of a Nizhny Novgorod infectious d...

Schuchmann, Karl-L., 2000: Taxonomy of Metallura. Reply. Journal fuer Ornithologie. April; 1412: 205-206