geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Recombinant bovine GM-CSF primes superoxide production but not degranulation induced by recombinant bovine interleukin-1 beta in bovine neutrophils


, : Recombinant bovine GM-CSF primes superoxide production but not degranulation induced by recombinant bovine interleukin-1 beta in bovine neutrophils. Journal of Leukocyte Biology 53(6): 679-684

Bovine neutrophil activation, superoxide production, and beta-glucosaminadase release induced by various biological stimuli were examined. Platelet-activating factor (PAF) and recombinant bovine interleukin-1 beta (r-BoIL-1 beta) induced superoxide production and beta-glucosaminadase release in bovine neutrophils. When these two responses were compared, the dose requirement for maximum activation was similar for PAF (1 x 10(-6) M). However, the concentration of r-BoIL-1 beta required for the maximum degranulation (2.5 x 10(-7) M) was 100-fold higher than that for the maximum superoxide production (2.5 x 10(-9) M). Furthermore, pretreatment of cells with recombinant bovine granulocyte-macrophage colony-stimulating factor (r-BoGM-CSF) enhanced both superoxide production and beta-glucosaminidase release induced by PAF. In contrast, whereas superoxide production induced by r-BoIL-1 beta was enhanced by r-BoGM-CSF priming, beta-glucosaminidase release induced by r-BoIL-1 beta was significantly reduced by pretreatment with r-BoGM-CSF. CL 184,005, a PAF antagonist, inhibited PAF-induced glucosaminidase release and superoxide production but did not inhibit r-BoIL-1 beta-induced superoxide production and degranulation. In addition, it did not inhibit the priming effect of r-BoGM-CSF on r-BoIL-1 beta-induced superoxide production. These results suggest that (1) PAF and r-BoIL-1 beta activate bovine neutrophils by different mechanisms, (2) r-BoGM-CSF primes superoxide production and degranulation induced by PAF, (3) r-BoGM-CSF primes superoxide production but not degranulation induced by r-BoIL-1 beta, and (4) the priming effect of r-BoGM-CSF is not mediated by PAF.

US$29.90

PMID: 8391055


Other references

Perkins, R.C.L., 1917: On the Kirby collection of Sphecodes, Nomada, Andrena and Cilissa with the description of a species of Sphecodes hitherto unrecorded from Britain. Entomologist's Monthly Magazine, 53 45-52

Gao, R.; Duan, K.; Guo, G.; Du, Z.; Chen, Z.; Li, L.; He, T.; Lu, R.; Huang, J., 2013: Comparative Transcriptional Profiling of Two Contrasting Barley Genotypes under Salinity Stress during the Seedling Stage. Salinity is one of the major abiotic stresses that affect crop productivity. Identification of the potential novel genes responsible for salt tolerance in barley will contribute to understanding the molecular mechanism of barley responses to salt...

Tkachenko, Z.Y. ; Demidova, G.G.; Chebotarev, E.E., 1978: Radiation response of the vascular wall endothelium. Radiation response of the vessel wall endothelium of the solar plexus, stomach and ileocecal region of the intestine of rabbits given a total-body X-ray exposure (300, 800 and 1400 R) was studied by light microscopy and EM. A dose of 300 R caused...

Thompson, M.B.; Chappell, J.D.; Kunze, D.J.; Blair, P.C., 1989: Bile acid profile in a dog with cholangiocarcinoma. Total serum bile acids were increased 100-fold in a dog with cholangiocarcinoma (382.79 micro moles/litre; normal 3.89 micro moles/litre). Of the total, 88.8% was taurocholic acid (339.92 micro moles/litre) which is characteristic of cholestasis....

López-Ribot, J.L.; Monteagudo, C.; Sepúlveda, P.; Casanova, M.; Martínez, J.P.; Chaffin, W.L., 1996: Expression of the fibrinogen binding mannoprotein and the laminin receptor of Candida albicans in vitro and in infected tissues. We have previously reported a 37 kDa laminin-binding protein (p37) and a 58 kDa fibrinogen-binding mannoprotein (mp58) on the surface of Candida albicans. A few yeast cells expressed both functional receptors at the surface while germ tubes expres...

Stebutt, A.I., 1946: Smonitsas of Serbia and the black soils of southern regions.. Smonitsa is a type of black soil formed originally under waterlogged conditions in depressions, later becoming well-drained or drying out due to change in climate. It is found mainly in the warmer latitudes. A suggested classification of black soi...

Kovacs T.; Parkanyi L.; Pelczer I.; Cervantes F.; Pannell K.H.; Torrence P.F., 1990: Solid state and solution conformation of 3 amino 3 deoxythymidine a noncompetitive inhibitor of hiv 1 reverse transcriptase. Abstracts of Papers American Chemical Society 200(1-2): CARB 36

Erickson, T.; Arora, A.; Lebby, T.I.; Lipscomb, J.W.; Leikin, J.B., 1991: Acute oral hypoglycemic ingestions. We reviewed the poison center records of 48 consecutive reports of oral hypoglycemic exposure reported to the Rush Poison Control Center between January 1988 and December 1989. The average age of ingestion was 15.0 y (range 1 to 75 y). Twenty-thre...

Heinemann, W.W.; Hanks, E.M.; Young, D.C., 1978: Monensin and tylosin in a high energy diet for finishing steers. In a 2 X 4 factorial design 167 Hereford, Aberdeen-Angus and Hereford X Aberdeen-Angus yearling bullocks weighing 355 kg were given for 127 days a diet of maize, dried sugar beet pulp with molasses, cubed lucerne hay, vitamins and trace elements a...

Anonymous, 1986: Single-patient trials. The Department of Clinical Epidemiology and Biostatistics at McMaster University has devised formal protocols for single-patient randomized trials to help clinicians find the optimal treatment for individual patients. Patients are allocated to a s...