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Reconstruction of transcriptional regulatory elements of human alpha-fetoprotein gene


, : Reconstruction of transcriptional regulatory elements of human alpha-fetoprotein gene. Zhongguo Yi Xue Ke Xue Yuan Xue Bao. Acta Academiae Medicinae Sinicae 20(2): 114-120

To construct vectors that express therapeutic genes specifically in hepatocellular carcinoma, the TREs (Tissue-specific transcriptional regulatory elements) of natural human alpha-fetoprotein (AFP) was reconstructed. Using subcloning techniques, the -870 bp(-)-3.06 kb fragment of the natural human AFP TREs was deleted to construct an artificial AFP(e/p) TREs which contains the "core" promoter and the -3.06 kb(-)-5.1 kb fragment of natural human AFP TREs that contains enhancer activity. By using chloramphenicol acetyltransferase (CAT) assay, the artificial AFP(e/p) TREs showed an activity 1.8 fold as high as that of the natural AFP TREs in an AFP positive human hepatocarcinoma cell line HepG2, and it also remained to be tissue-specific since there was no activity was detected in an AFP negative human larynxcarcinoma cell line Hep2. These results indicate that the artificial AFP(e/p) TREs we have constructed has higher activity than the natural AFP TREs and it also remains its hepatocarcinoma specificity. The reconstructed AFP(e/p) TREs is suitable for constructing of gene therapy vector with hepatocarcinoma expression specificity.

US$29.90

PMID: 11367716


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Hankins, C.L.; Friedman, J., 2008: A 7-year experience in utilizing the latissimus dorsi myocutaneous flap for bilateral breast reconstruction. Due to the benefits of prophylactic mastectomy in women at risk of breast cancer or recurrent disease, we have noted an increase in the number of women presenting for bilateral breast reconstruction. Current dogma stipulates that flaps of abdomina...

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Kursakov, G.A., 1980: Development of studies on the genetics and breeding of fruit crops at the I. V. Michurin Central Genetics Laboratory. In this outline of recent work at the Michurin Laboratory, particular attention is paid to interspecific and intergeneric hybrids and the methods used in their production, notably interspecific hybrids of black currant and various stone fruits and...

Comp, P.C., 1984: Animal studies of protein C physiology. Seminars in Thrombosis and Hemostasis 10(2): 149-153

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Daffonchio A.; Franzelli C.; Mancia G.; Ferrari A.U., 1991: Cardiac muscarinic receptor hyperresponsiveness in spontaneously hypertensive rats. European Journal of Clinical Investigation 21(2 PART 2): 14

Anon., 1905: Revised list of casts, models, photographs and restorations of fossil vertebrates. Bulletin of the American Museum, Vol. XX 1-52

Murray, P.; Edgar, D., 2004: The topographical regulation of embryonic stem cell differentiation. The potential use of pluripotent stem cells for tissue repair or replacement is now well recognized. While the ability of embryonic stem (ES) cells to differentiate into all cells of the body is undisputed, their use is currently restricted by our...

Greiner, G.F., 1974: Deafness, sequelae of skull injuries. La Revue du Praticien 24(31): 2839-2843

van Belle, G.; Uhlmann, R.F.; Hughes, J.P.; Larson, E.B., 1990: Reliability of estimates of changes in mental status test performance in senile dementia of the Alzheimer type. The concept of the reliability of a measure can also be applied to its change over time. In this study we consider the growth curve approach to estimating the reliability of change, in the context of cognitive status as measured by the Mini-Mental...