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Regulation of PKC alpha activity by C1-C2 domain interactions


, : Regulation of PKC alpha activity by C1-C2 domain interactions. Journal of Biological Chemistry 277(18): 15277-15285

In this study, the role of interdomain interactions involving the C1 and C2 domains in the mechanism of activation of PKC was investigated. Using an in vitro assay containing only purified recombinant proteins and the phorbol ester, 4 beta-12-O-tetradecanoylphorbol-13-acetate (TPA), but lacking lipids, it was found that PKC alpha bound specifically, and with high affinity, to a alpha C1A-C1B fusion protein of the same isozyme. The alpha C1A-C1B domain also potently activated the isozyme in a phorbol ester- and diacylglycerol-dependent manner. The level of this activity was comparable with that resulting from membrane association induced under maximally activating conditions. Furthermore, it was found that alpha C1A-C1B bound to a peptide containing the C2 domain of PKC alpha. The alpha C1A-C1B domain also activated conventional PKC beta I, -beta II, and -gamma isoforms, but not novel PKC delta or -epsilon. PKC delta and -epsilon were each activated by their own C1 domains, whereas PKC alpha, -beta I, -beta II, or -gamma activities were unaffected by the C1 domain of PKC delta and only slightly activated by that of PKC epsilon. PKC zeta activity was unaffected by its own C1 domain and those of the other PKC isozymes. Based on these findings, it is proposed that the activating conformational change in PKC alpha results from the dissociation of intra-molecular interactions between the alpha C1A-C1B domain and the C2 domain. Furthermore, it is shown that PKC alpha forms dimers via inter-molecular interactions between the C1 and C2 domains of two neighboring molecules. These mechanisms may also apply for the activation of the other conventional and novel PKC isozymes.

US$19.90

PMID: 11850425

DOI: 10.1074/jbc.M112207200


Other references

Webb, R.; Abel, K.; Pickles, A.; Appleby, L., 2005: Mortality in offspring of parents with psychotic disorders: a critical review and meta-analysis. Objective: Mortality risk in offspring of parents with psychotic disorders is a sensitive and important topic, but evidence on which to base plans for preventive services is limited. The authors synthesized evidence for mortality risk among offspr...

Lagacé, M.; Xuan, J.Y.; Young, S.S.; McRoberts, C.; Maier, J.; Rajcan-Separovic, E.; Korneluk, R.G., 2001: Genomic organization of the X-linked inhibitor of apoptosis and identification of a novel testis-specific transcript. Here we report the genomic organization and mapping of the X-linked inhibitor of apoptosis gene (BIRC4, also known as XIAP and hILP) and the identification of a closely related transcript. BIRC4 is located on Xq25 and is composed of seven exons. T...

Pamra, S.P., 1983: Haemoptysis in chest diseases. Indian Journal of Chest Diseases & Allied Sciences 25(4): 249-25l

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Ahren, B.; Wierup, N.; Sundler, F., 2006: Neuropeptides and the regulation of islet function. The pancreatic islets are richly innervated by autonomic nerves. The islet parasympathetic nerves emanate from intrapancreatic ganglia, which are controlled by preganglionic vagal nerves. The islet sympathetic nerves are postganglionic with the n...

Day R.S.IIi; Rupert C.S., 1971: Uv sensitivity of haemophilus influenzae transforming dna part 1 effects of genetic mismatch and target size. Mutation Research 11(3): 293-311

YaredAssefa; DerejeHabte; TegbarYigzaw; AlemayehuMekonren; TeshomeGebrie; TewodrosDubale; MulatZerihun, 2008: Trichiasis recurrence in north Ethiopia a one year prospective study of trachomatous trichiasis surgery done by integrated eye care workers. Background: Blindness due to trachomatous trichiasis can be prevented by simple eyelid surgery that can be performed by trained nurses. Objective: To evaluate the post-operative outcome of posterior tarsal rotation done by integrated eye care work...

Vigneron J.P.; Mourareau C.; Guisset M., 1990: Medical and physiological aspects of survival on land and sea after an air crash. Every situation of survival on land or on sea after an air crash is characterized by some different medical and physiological aspects. Their knowledge by crew members and the apprenticeship of methods of survival with adapted kits make indispensab...

Smith, E.F.; Jones, L.R.; Reddy, C.S., 1919: The Black Chaff Of Wheat. Science 50(1280): 48-48

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