geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Role of one N-linked oligosaccharide chain on canine herpesvirus gD in its biological activity


, : Role of one N-linked oligosaccharide chain on canine herpesvirus gD in its biological activity. Journal of Veterinary Medical Science 59(12): 1123-1128

The YP11mu strain of a plaque-selected canine herpesvirus (CHV) encoded a smaller molecular weight (MW) of gD than those of other strains including YP2 strain (Xuan et al., 1990). When nucleotide sequence of the mutated gD of YP11mu strain (gD(YP11mu)) was compared with that of gDs of other CHV strains, gD(YP11mu) lacked 12 nucleotides encoding 4 amino acids, NKTI, including one predicted potential N-linked glycosylation site and no other change was found in other regions. When the gD(YP11mu) and gD of YP2 strain (gD(YP2)) expressed in COS-7 and insect (Spodoptera frugiperda; Sf9) cells were compared each other, both gDs reacted with a panel of monoclonal antibodies (MAbs) against CHV gD by indirect immunofluorescence analysis and the gD(YP11mu) possessed an MW of approximately 47-51 and 39-44 kDa in COS-7 and Sf9 cells, respectively, which were smaller than the expressed gD(YP2) (approximately 51-55 and 41-46 kDa, respectively) by immunoblot analysis. After treatment with tunicamycin, the MW of both gDs in Sf9 cells became approximately 37 kDa. When hemagglutination (HA) test using canine red blood cells (RBC) were carried out, lysates of Sf9 cells expressing CHV gDs agglutinated canine RBC. Serum from mice inoculated with lysates of Sf9 cells expressing the gDs possessed a high titer of virus-neutralizing (VN) activities against CHV. These results indicated that the deletion of 4 amino acids possessing approximately 4 kDa of glyco-chain from gD of CHV in mammalian cells does not affect HA activity and VN antibody-inducing activity and that this deletion of gD(YP11mu) might be a good selective marker for development of recombinant viruses as a live vaccine.

US$29.90

PMID: 9450242


Other references

Rodrigues, C.Ewerton.Maia.; Callado, M.Roseli.Monteiro.; Nobre, C.Aguiar.; Moura, F.Edwiges.Araújo.; Vieira, R.Maria.Rodrigues.de.Abreu.; de Albuquerque, L.Alverne.Freitas.; Vieira, W.Pinto., 2011: Wegener's granulomatosis: prevalence of the initial clinical manifestations--report of six cases and review of the literature. To describe the initial clinical manifestations of Wegener's Granulomatosis (WG) in Brazil. Retrospective analysis of six medical records of WG patients followed-up at the Rheumatology Department of Hospital Geral of Fortaleza (HGF), as well...

Liebman, J., 1990: Use of noninvasively determined right ventricular epicardial breakthrough in clinical diagnosis and understanding. The researchers have presented a series of patients using their 180-electrode system with color mapping, in which the presence of RVBT either provided evidence for diagnosis or gave insight into anatomy and/or pathophysiology. This noninvasive too...

Fireman, Z.; Kopelman, Y.; Friedman, S.; Ephrath, H.; Choman, E.; Debby, H.; Eliakim, R., 2007: Age and indication for referral to capsule endoscopy significantly affect small bowel transit times: the given database. The purpose of this study was to examine the effect of age and selected indications for capsule endoscopy on small bowel transit times. Data on 67 clinical studies (790 subjects with different gastrointestinal pathologies [49.5% males; mean age, 5...

Gardner, M.; Yamamoto, J.; Marthas, M.; Miller, C.; Jennings, M.; Rosenthal, A.; Luciw, P.; Planelles, V.; Yilma, T.; Giavedoni, L., 1992: SIV and FIV vaccine studies at UC Davis: 1991 update. Aids Research and Human Retroviruses 8(8): 1495-1498

Zhou XiaoMei; L.J.nJian; Zhao JunLiang; Liang AiHua, 2005: Effects of antibiotics on inhibition of Agrobacterium tumefaciens and differentiation of leaf stems from Brassica juncea. Nine antibiotics (pinperacillin, ampicillin, panicillin sodium, carbenicillin, cefradine, cefzolin sodium, fosfomycin, erythromycin and leacomycinum) were used to eliminate Agrobacterium tumefaciens EHA105 and LBA4404 during plant transformation....

Renzoni A., 1976: Marine parks and reserves in italy. Environmental Conservation 3(2): 131-135

Nomura, O.; Nakabayashi, O.; Nishimori, K.; Yasue, H.; Mizuno, S., 2000: Expression of five steroidogenic genes including aromatase gene at early developmental stages of chicken male and female embryos. In the course of avian embryo development, estrogen has been indicated to play a key role in gonadal differentiation by the inhibition of aromatase (P-450arom) that synthesizes estrogen from androgen. Biosynthesis of estrogen requires not only P-4...

Al Salloum, H.; Saunier, J.; Dazzi, A.; Vigneron, J.; Etcheberry, A.; Marlière, C.; Aymes-Chodur, C.; Herry, J.M.; Bernard, M.; Jubeli, E.; Yagoubi, N., 2017: Characterization of the surface physico-chemistry of plasticized PVC used in blood bag and infusion tubing. Commercial infusion tubing and blood storage devices (tubing, blood and platelets bags) made of plasticized PVC were analyzed by spectroscopic, chromatographic and microscopic techniques in order to identify and quantify the additives added to the...

Yang, CC.; Lee, HJ., 1986: Selective modification of tyrosine-68 in 1-bungarotoxin from the venom of Bungarus multicinctus (Taiwan banded krait). Journal of Protein Chemistry, 51: 15-28

Blanc, C.P., 1978: Notes on tunisian reptiles part 1 contribution to the study of the genera ophisops and psammodromus reptilia lacertidae. The distribution area, the nature of the biotopes and the morphological and biometrical features of O. occidentalis, P. blanci and P. algirus algirus are reported. Similarity in the morphology and coloration of the first 2 spp. is analyzed togethe...