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The changes of protein kinase C activity in peripheral blood lymphocytes in asthmatic patients

, : The changes of protein kinase C activity in peripheral blood lymphocytes in asthmatic patients. Zhonghua Jie He He Hu Xi Za Zhi 22(8): 461-464

To investigate changes of protein kinase C (PKC) activities in peripheral blood lymphocytes (PBL) and the relationship between PKC activities and level of the air flow limitation in asthmatic patients. PKC from cytosolic and membrane fractions of PBL was isolated and purified, then its activities were determined. (1) The total PKC activities in PBL in asthmatic subjects (n = 18) were significantly increased as compared to those in normal subjects (n = 6, P < 0.01). More over, the membrane PKC activities and their percentages in the total PKC activities were higher in asthmatics than in normals (P < 0.05). In vitro it suggested that methacholine(n = 5) or histamine (n = 5) could significantly increase translocation of PKC from cytosol to membrane (P < 0.05) and, by doing so, activate the PKC in PBL. (2) The total PKC activities were significantly correlated with FEV1% (r = -0.73, P < 0.001, n = 18), PEF(r = -0.62, P < 0.01, n = 18) and V50 (r = -0.63, P < 0.01, n = 18). (3) The total PKC activities in PBL were significantly correlated with the levels of sIL-2R in serum in the asthmatics (r = 0.58, P < 0.05, n = 18). (1) The total activities and the level of translocation of PKC were significantly increased in PBL in asthmatics as compared to normals. This abnormal activation state of PKC in PBL may be related with the increased levels of inflammatory mediators. (2) Highly significant correlations existed between the total PKC activities and the level of air flow limitation in asthmatics. (3) PKC signal pathway may be implicated in the activation of T-lymphocytes in asthma. It is suggested that the role of PKC signal pathway may be important in the pathogenisis of asthma.


PMID: 11776558

Other references

Oshima, Junko, 1999: Functional inactivation of WRN in mice. FASEB Journal 13(7): A1328, April 23

Kosar, E.; Chandraratna, P.A.thony, 1997: Assessment of coronary artery aneurysms with multiplane transesophageal echocardiography. Coronary artery aneurysms are rare and may be difficult to detect clinically. Multiplane transesophageal echocardiography provides numerous imaging planes that may improve the assessment of coronary aneurysms and act as an adjunct to standard angi...

Anson, J.G., 1982: Memory drum theory: alternative tests and explanations for the complexity effects on simple reaction time. Two experiments investigated the memory drum theory's prediction (Henry & Rogers, 1960) that simple reaction time (SRT) increased with the complexity of the response to be initiated. Experiment 1 (N = 9) matched the Experiment 1, Group 1,...

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Rosenberg, C.M., 1970: Forearm blood flow in response to stress. Journal of Abnormal Psychology 76(2): 180-184

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Jaeger U.; Lohoff U.; Bach D.; Schopka H J., 1990: Hmpao spect quantification by automatic contour line analysis. European Journal of Nuclear Medicine 16(7): 394

Manolopoulos, D.E.; Stark, K.; Werner, H.J.; Arnold, D.W.; Bradforth, S.E.; Neumark, D.M., 1993: The transition state of the f + h2 reaction. The transition state region of the F + H(2) reaction has been studied by photoelectron spectroscopy of FH(2)(-). New para and normal FH(2)(-)photoelectron spectra have been measured in refined experiments and are compared here with exact three-dim...

Mall-haefeli, M.; Ludwig, K.S.; Keller, M.; Weber, W., 1969: Contribution on the mode of action of oral ovulation inhibitors in humans. Report 2. 5 women who had taken various ovulation inhibitors for varying duration (3-20 cycles) underwent sterilization in the 1st, 2nd, or 3rd cycle after discontinuing treatment. Urine and tissue samples were taken. Analysis showed that pregnanediol level...

Hancock, G.E.; Speelman, D.J.; Frenchick, P.J., 1998: Adjuvants for vaccines against respiratory syncytial virus. Official Gazette of the United States Patent & Trademark Office Patents 1208(1): 360, March 3