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The role of alpha and beta chains in ligand recognition by beta 7 integrins

, : The role of alpha and beta chains in ligand recognition by beta 7 integrins. Journal of Biological Chemistry 275(33): 25652-25664

Integrins alpha(E)beta(7) and alpha(4)beta(7) are involved in localization of leukocytes at mucosal sites. Although both alpha(E)beta(7) and alpha(4)beta(7) utilize the beta(7) chain, they have distinct binding specificities for E-cadherin and mucosal addressin cell adhesion molecule-1 (MAdCAM-1), respectively. We found that mutation of the metal ion-dependent adhesion site (MIDAS) in the alpha(E) A-domain (D190A) abolished E-cadherin binding, as did mutation F298A on the A-domain surface near the MIDAS cleft. A docking model of the A-domain with E-cadherin domain 1 indicates that coordination of the alpha(E) MIDAS metal ion by E-cadherin Glu(31) and a novel projection of Phe(298) into a hydrophobic pocket on E-cadherin provide the basis for the interaction. The location of the binding site on the alpha(E) A-domain resembles that on other integrins, but its structure appears distinctive and particularly adapted to recognize the tip of E-cadherin, a unique integrin ligand. Additionally, mutation of the beta(7) MIDAS motif (D140A) abolished alpha(E)beta(7) binding to E-cadherin and alpha(4)beta(7)-mediated adhesion to MAdCAM-1, and alpha(4) chain mutations that abrogated binding of alpha(4)beta(1) to vascular cell adhesion molecule-1 and fibronectin similarly reduced alpha(4)beta(7) interaction with MAdCAM-1. Thus, although specificity can be determined by the integrin alpha or beta chain, common structural features of both subunits are required for recognition of dissimilar ligands.


PMID: 10837471

DOI: 10.1074/jbc.M001228200

Other references

Tarczynski, Stefan, 1960: The parasitological institutes in Koszyce. Wiadomosci Parazytol 6(6): 543-547

Tagari, P.C.; Boullin, D.J.; Davies, C.L., 1984: Simplified determination of serotonin in plasma by liquid chromatography with electrochemical detection. This simplified determination of serotonin in human plasma involves "high-performance" liquid chromatography with electrochemical detection. Deproteinated samples of platelet-poor plasma are injected directly into the chromatograph witho...

Sellier, P.; Lopes, A.; Bergmann, J-François., 2015: Vertical transmission of hepatitis B virus. Annals of Internal Medicine 161(10): 762-763

Ibrahim, I.; Joshi, M.; Hollis, J., 1975: Respiration measurements of four nematode species with the biological oxygen monitor. Alexandria journal of agricultural research: 23 (1) 161-165

Laux, E-Maria.; Kaletta, U.C.; Bier, F.F.; Wenger, C.; Hölzel, R., 2014: Functionality of dielectrophoretically immobilized enzyme molecules. The enzyme horseradish peroxidase has been immobilized on nanoelectrode arrays by alternating current dielectrophoresis (DEP). Preservation of its enzymatic function after field application was demonstrated by oxidizing dihydrorhodamine 123 with h...

Vines, C.R.; Braid, M.R.; Raymond, C.B.; Sanders, W.S., 1996: A collapsible cage design for aquatic culture. The design and construction of a multi-role aquaculture cage is described. The cage was designed with consideration of role flexibility, survivability, resistance to the elements, stability, transportability, construction simplicity, inherent non-...

Fraser, F.C., 1946: Hylaeothemis indica, a new species of oriental Libellulino (Order Odonata). Proceedings of the Royal Entomological Society of London (B): 15: 97-100

Delphaut, J.; Bel, L., 1952: Action of the sodium salts of salicylic acid, gentisic acid, p-aminosalicylic acid benzoly-p-aminosalicylic acid on choleresis diuresis in the dog under chloralose anesthesia. Comptes Rendus des Seances de la Societe de Biologie et de Ses Filiales 146(15-16): 1214-1216

Trittmann K., 1972: Ornithological studies in selected biotopes in the area huettental westphalia. Natur und Heimat 32(1): 17-20

Hessling, J., 1979: The husbandry of young chickens. Fjaderfa 72(3): 92-93