+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Thermodynamics and Structural Studies of the Interaction of Polymyxin B with Deep Rough Mutant Lipopolysaccharides

, : Thermodynamics and Structural Studies of the Interaction of Polymyxin B with Deep Rough Mutant Lipopolysaccharides. Journal of Colloid and Interface Science 213(2): 557-564

Deep rough mutant lipopolysaccharide (ReLPS) dissolved in aqueous solution spontaneously forms supramolecular structures which mainly consist of vesicles. Addition of Polymyxin B (PmB) to these ReLPS vesicles influence the shape of these structures as demonstrated here by electronmicroscopy and dynamic light scattering techniques. The main phase transition of the ReLPS is found at 21.3 +/- 0.1 degrees C for ReLPS from Escherichia coli and at 24.0 +/- 0.5 degrees C for ReLPS from Salmonella minnesota by differential scanning calorimetry (DSC). Using isothermal differential titration calorimetry (ITC), the thermodynamic behavior of the interaction of PmB with ReLPS vesicles has been studied. The stoichiometric ratio for the binding of PmB to ReLPS was found to lie between 0.6 and 1, as determined from ITC and monolayer experiments. No phase transition was observed for ReLPS monolayers saturated with PmB. The results indicate specific interaction of PmB with ReLPS. We propose a two-step mechanism for this interaction, which involves electrostatic attraction between charged parts of the molecules and, in the second step, hydrophobic interactions between the nonpolar parts of both compounds. Copyright 1999 Academic Press.


PMID: 10222097

DOI: 10.1006/jcis.1999.6137

Other references

Miyatani, S.; Hiramatsu, K.; Nakajima, P.B.; Owen, F.L.; Tada, T., 1983: Structural analysis of antigen-specific Ia-bearing regulatory T-cell factors: gel electrophoretic analysis of the antigen-specific augmenting T -cell factor. An antigen-specific T-cell factor (TaF) that specifically augments the antibody response was purified and biochemically analyzed by NaDodSO4/polyacrylamide gel electrophoresis and isoelectric focusing. Biosynthetically labeled TaF was separated fr...

Taddei, M., 1968: A case of recurrent asymptomatic cystic thymoma. Schweizerische Medizinische Wochenschrift 98(36): 1354-1357

Duchini, L., 1966: On sarcoma of the abdominal wall. Archivio "de Vecchi" Per L'anatomia Patologica E la Medicina Clinica 48(1): 301-310

Calabro S.; Martines F.; Pugliatti V.; Napoli P.; Oteri G.; Lucifora G.; Magaudda D.; Gullo G., 1987: Immunohistochemical study of the igg in the placentas of asymptomatic hbsag carriers. In the present study we marked the IgG fixed at the placental sites of asymptomatic HBsAg carrier-mothers by immunoperoxidasic method in order to estimate the possible changes in their distribution rate and compared the results with normal placent...

Montanaro, L.; Sperti, S.; Mattioli, A.; Testoni, G.; Sterpe, F., 1975: Inhibition by ricin of protein synthesis in vitro inhibition of the binding of elongation factor 2 and of adp ribosylated elongation factor 2 to ribosomes. Biochemical Journal 146(1): 127-131

Arntzen F.C., 1968: Determination of pre kallikrein in human plasma kinin release rat uterus. Acta Pharmacologica Et Toxicologica: 395-401

Medrano, C.; Mendt, R.; Rasmussen, A., 1976: New herbicides for weed control in onions. Of the 8 herbicides applied one week after transplanting, good weed control and significant yield increase were obtained with RH-2915 [oxyfluorfen] at 2 litres/ha, Cloro IPC (chlorpropham) at 10 litres/ha, AC-92553 [pendimethalin] at 4 litres/ha o...

Cantrell, J.D.; Dana, R.H., 1987: Use of the Millon Clinical Multiaxial Inventory (MCMI) as a screening instrument at a community mental health center. Comparison of the Millon Clinical Multiaxial Inventory (MCMI) and clinician-generated DSM-III diagnoses for a sample of psychiatric outpatients (N = 72) indicated only chance agreement. The MCMI was of limited use as a screening device because of...

Eldredge, W.J.; Tingelstad, J.B.; Robertson, L.W.; Mauck, H.P.; McCue, C.M., 1972: Observations on the natural history of pulmonary artery coarctations. Circulation 45(2): 404-409

Balamuralikrishnan, M.; Sabitha Doraisamy; Ganapathy, T.; Viswanathan, R., 2005: Effects of biotic and abiotic agents on Sugarcane mosaic virus titre, oxidative enzymes and phenolics in Sorghum bicolor. Greenhouse experiments were conducted to study the effects of various biotic (Pseudomonas fluorescens) and abiotic agents (acibenzolar-S-methyl and salicylic acid), applied via seed treatment, soil application and foliar spray, in inducing systemi...