+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Acute intermittent hypoxia activates myocardial cell survival signaling

, : Acute intermittent hypoxia activates myocardial cell survival signaling. American Journal of Physiology. Heart and Circulatory Physiology 292(2): H751-H757

Intermittent hypoxia (IH) with repeated episodes of hypoxia-normoxia cycle has been shown to exert preconditioning-like cardioprotective effects. To understand the mechanism of these events, we investigated the changes in cardiac gene expression in response to acute IH. Mice were subjected to five cycles of 2 min of 10% O(2) plus 2 min of 21% O(2). RNA was isolated, and gene array analysis was performed. Results show that the expression of antiapoptotic genes, such as Bcl-2 and Bcl-x(L), were increased after acute IH. GATA-4 regulates transcription of these genes, and, consistently, GATA-4 activity was increased by acute IH. Although the phosphorylation of GATA-4 has been shown to regulate its activity, no changes in GATA-4 phosphorylation status by acute IH were noted. Gene transcription of gata4 was increased by acute IH, and this might be responsible for the enhanced GATA activity. To understand the mechanism of acute IH activation of gata4 gene transcription, we identified a promoter region of the mouse gata4 gene that is 1,000 bp immediately upstream from the transcriptional start site. In cardiac muscle cells, truncation of 1,000 to 250 bp did not alter the transcriptional activity, suggesting that the proximal 250-bp region contains important transcriptional regulatory sites. We further found that acute IH activates factors which bind to the proximal 100-bp region. Thus acute IH activates not yet identified factors that bind to the proximal 100-bp region of the gata4 promoter and, in turn, increases gata4 gene transcription, leading to enhanced expression of Bcl-2 and Bcl-x(L).


PMID: 17098826

DOI: 10.1152/ajpheart.01016.2006

Other references

Fisher, J., 1956: Rockall. Unknown

Preibisch, J., 1979: Investigations on the intestinal plasma cells in spontaneous colibacteriosis of pigs. Polskie archiwum weterynaryjne = Archivum veterinarium polonicum1(2): 257-264

Craig, J.M.; Hawkins, J.M.; Yamada, T.; Ganeshaguru, K.; Mehta, A.B.; Secker-Walker, L.M., 1990: First intron and M-bcr breakpoints are restricted to the lymphoid lineage in Philadelphia positive acute lymphoblastic leukemia. Knowledge of the level of commitment of the target cell in hematological malignancies may have important therapeutic and prognostic implications. Cell lineage involvement was investigated in two cases presenting with acute lymphoblastic leukemia d...

Benbow, S.Z.; DuBois, M.L., 2008: The dosage of chromatin proteins affects transcriptional silencing and DNA repair in Saccharomyces cerevisiae. Alterations in protein composition or dosage within chromatin may trigger changes in processes such as gene expression and DNA repair. Through transposon mutagenesis and targeted gene deletions in haploids and diploids of Saccharomyces cerevisiae...

Ochsner, F., 1958: Changes in bryophyte vegetation in the experimental area Schinig platte, Bernese Oberland, 1945-1954. Plots in the experimental area are fertilized and harvested according to various principles, in order to elucidate the development of alpine meadows under cultivation conditions. The development of the moss cover is followed by means of permanent...

Thurnham, D.I.; Oppenheimer, S.J.; Bull, R., 1983: Riboflavin status and malaria in infants in Papua New Guinea. Riboflavin deficiency in infants may be protective against malaria: Papua New Guinea

Fine, M.L.; Mcelroy, D.; Rafi, J.; King, C.B.ian; Loesser, K.E.; Newton, S., 1996: Lateralization of pectoral stridulation sound production in the channel catfish. Sounds of the channel catfish Ictalurus punctatus were found to consist of a rapid series of pulses produced by rubbing a ridged process on the first pectoral spine against the rough surface of a groove in the pectoral girdle during fin abduction....

Halle, M.Kyllesø.; Werner, H.Maria.Johanna.; Krakstad, C.; Birkeland, E.; Wik, E.; Trovik, J.; Salvesen, H.B., 2012: Stratification based on high tumour cell content in fresh frozen tissue promotes selection of aggressive endometrial carcinomas. Histopathology 60(3): 516-519

Hager S.W.; Harmon D.D.; Alpine A.E., 1984: Chemical determination of particulate nitrogen in san francisco bay california usa nitrogen chlorophyll a ratios in plankton. Particulate nitrogen (PN) and chlorophyll a (Chla) were measured in the northern reach of San Francisco Bay throughout 1980. The PN values were calculated as the differences between unfiltered and filtered (0.4 .mu.m) samples analyzed using the UV...

Merkel, J.A.; Heldman, D.R.Reviewer, 1984: Basic engineering principles (book review). Food Technology 38: 0-1