geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Analysis of Bartonella adhesin A expression reveals differences between various B. henselae strains


, : Analysis of Bartonella adhesin A expression reveals differences between various B. henselae strains. Infection and Immunity 75(1): 35-43

Bartonella henselae causes cat scratch disease and the vasculoproliferative disorders bacillary angiomatosis and peliosis hepatis in humans. One of the best known pathogenicity factors of B. henselae is Bartonella adhesin A (BadA), which is modularly constructed, consisting of head, neck/stalk, and membrane anchor domains. BadA is important for the adhesion of B. henselae to extracellular-matrix proteins and endothelial cells (ECs). In this study, we analyzed different B. henselae strains for BadA expression, autoagglutination, fibronectin (Fn) binding, and adhesion to ECs. We found that the B. henselae strains Marseille, ATCC 49882, Freiburg 96BK3 (FR96BK3), FR96BK38, and G-5436 express BadA. Remarkably, BadA expression was lacking in a B. henselae ATCC 49882 variant, in strains ATCC 49793 and Berlin-1, and in the majority of bacteria of strain Berlin-2. Adherence of B. henselae to ECs and Fn reliably correlated with BadA expression. badA was present in all tested strains, although the length of the gene varied significantly due to length variations of the stalk region. Sequencing of the promoter, head, and membrane anchor regions revealed only minor differences that did not correlate with BadA expression, apart from strain Berlin-1, in which a 1-bp deletion led to a frameshift in the head region of BadA. Our data suggest that, apart from the identified genetic modifications (frameshift deletion and recombination), other so-far-unknown regulatory mechanisms influence BadA expression. Because of variations between and within different B. henselae isolates, BadA expression should be analyzed before performing infection experiments with B. henselae.

US$19.90

PMID: 17060468

DOI: 10.1128/IAI.00963-06


Other references

Lustik, S.J.; Papadakos, P.J.; Jackman, K.V.; Rubery, P.T.; Kaplan, K.L.; Chhibber, A.K., 2004: Nicardipine versus nitroprusside for deliberate hypotension during idiopathic scoliosis repair. To compare coagulation, blood loss, and transfusion requirements between patients receiving nicardipine and nitroprusside for deliberate hypotension during idiopathic scoliosis repair. Prospective, randomized study. University hospital. 51 ASA phy...

Woropajew, L.; Rewuzkij, L., 1977: Organization of technical servicing of heavy transport vehicles in agriculture. The USSR organization for servicing farm machinery at the farm, local, and central depot levels is described, some examples are given of its working, and plans for further improvement are outlined.

Pommerening, A.; Lewandowski, A., 1997: Simulating alternative sampling strategies for heterogeneous mixed stands. A new approach to the simulation of sampling methods in inventoried, mixed, uneven-aged forest stands is presented. The results gained using the method contain not only inventory data but also comprehensive statistical information: this permits th...

Anonymous, 2002: Charles C. Culpeper scholarships in medical science. Developmental Biology 247(1): 4

Sevdali, M.; Kumar, V.; Peckham, M.; Sparrow, J., 2013: Human congenital myopathy actin mutants cause myopathy and alter Z-disc structure in Drosophila flight muscle. Over 190 mutations in the human skeletal muscle ?-actin gene, <italic>ACTA1</italic> cause congenital actin myopathies. We transgenically expressed six different mutant actins, G15R, I136M, D154N, V163L, V163M and D292V in <italic&g...

Lovelady, G.K.; Mirro, R.; Armstead, W.M.; Busija, D.W.; Leffler, C.W., 1988: Effect of 15-HETE on cerebral arterioles of newborn pigs. Effects of topical application of 15-HETE on pial arteriolar diameter and cortical periarachnoid cerebrospinal fluid (CSF) prostanoid concentrations were investigated in chloralose-anesthetized newborn pigs. Pial arteriolar diameters were measured...

Allam, J-Pierre.; Würtzen, P.A.; Reinartz, M.; Winter, J.; Vrtala, S.; Chen, K-Wei.; Valenta, R.; Wenghoefer, M.; Appel, T.; Gros, E.; Niederhagen, B.; Bieber, T.; Lund, K.; Novak, N., 2010: Phl p 5 resorption in human oral mucosa leads to dose-dependent and time-dependent allergen binding by oral mucosal Langerhans cells, attenuates their maturation, and enhances their migratory and TGF-beta1 and IL-10-producing properties. Sublingual immunotherapy (SLIT) is safe and effective as treatment of allergic rhinitis and mild asthma. Oral mucosal Langerhans cells (oLCs) play a central role. However, little is known about allergen binding by oLCs during mucosal allergen reso...

Labzyuk, VI., 1988: White-breasted water hen (Amaurornis phoenicurus) - genus and species new for the fauna of the USSR. Zoologicheskii Zhurnal, 674: 630

Knecht, K.T.; Thurman, R.G.; Mason, R.P., 1993: Role of superoxide and trace transition metals in the production of alpha-hydroxyethyl radical from ethanol by microsomes from alcohol dehydrogenase-deficient deermice. The formation of alpha-hydroxyethyl radical from ethanol by deermouse microsomes supplemented with NADPH has been demonstrated with the EPR technique of spin trapping with alpha-(4-pyridyl-1-oxide)-N-t-butylnitrone (POBN) as the spin trap, in the...

Tanimoto, T.E.; Saito, A.; Kami, M.; Kanda, Y.; Ando, T.; Nakai, K.; Saito, T.; Kataoka, I.; Kim, S.W.n; Ohnishi, M.; Tanosaki, R.; Kobayashi, Y.; Tobinai, K.; Mineishi, S.; Takaue, Y., 2001: Comparison of chronic graft-versus-host disease following allogeneic bone marrow vs peripheral blood stem cell transplantation in Japanese patients. Recently, PBSC has become more frequently used in place of BM in allogeneic transplantation. PBSC may provide benefits, which include faster hematoimmune reconstitution and enhanced immune-mediated antileukemia effects, that may or may not be asso...