+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Analysis of mutations and bone marrow micronuclei in Big Blue rats fed leucomalachite green

, : Analysis of mutations and bone marrow micronuclei in Big Blue rats fed leucomalachite green. Mutation Research 547(1-2): 5-18

Leucomalachite green (LMG) is the major metabolite of malachite green (MG), a triphenylmethane dye that has been used widely as an antifungal agent in the fish industry. Concern over MG and LMG is due to the potential for consumer exposure, suggestive evidence of tumor promotion in rodent liver, and suspicion of carcinogenicity based on structure-activity relationships. In order to evaluate the risks associated with exposure to LMG, female Big Blue rats were fed up to 543 ppm LMG; groups of these rats were killed after 4, 16, or 32 weeks of exposure and evaluated for genotoxicity. We previously reported that this treatment resulted in a dose-dependent induction of liver DNA adducts, and that the liver lacI mutant frequency (MF) was increased, but only in rats fed 543 ppm LMG for 16 weeks. In the present study, we report the results from lymphocyte Hprt mutant assays and bone marrow micronucleus assays performed on these same rats. In addition, we have determined the types of lacI mutations induced in the rats fed 543 ppm LMG for 16 weeks and the rats fed control diet. No significant increases in the frequency of micronuclei or Hprt mutants were observed for any of the doses or time points assayed. Molecular analysis of 80 liver lacI mutants from rats fed 543 ppm LMG for 16 weeks revealed that 21% (17/80) were clonal in origin and that most (55/63) of the independent mutations were base pair substitutions. The predominant type of mutation was G:C --> A:T transition (31/63) and the majority (68%) of these involved CpG sites. When corrected for clonality, the 16-week lacI mutation frequency (36 +/- 10) x 10(-6) in treated rats was not significantly different from the clonally corrected control frequency (17 +/- 9 x 10(-6); P = 0.06). Furthermore, the lacI mutational spectrum in treated rats was not significantly different from that found for control rats (P = 0.09). Taken together, these data indicate that the DNA adducts produced by LMG in female rats do not result in detectable levels of genotoxicity, and that the increase in lacI MF observed previously in the liver of treated rats may be due to the disproportionate expansion of spontaneous lacI mutations.


PMID: 15013694

DOI: 10.1016/j.mrfmmm.2003.11.009

Other references

Vercellino, M.; Grifoni, S.; Romagnolo, A.; Masera, S.; Mattioda, A.; Trebini, C.; Chiavazza, C.; Caligiana, L.; Capello, E.; Mancardi, G.Luigi.; Giobbe, D.; Mutani, R.; Giordana, M.Teresa.; Cavalla, P., 2012: Progranulin expression in brain tissue and cerebrospinal fluid levels in multiple sclerosis. Progranulin (PGRN) is a fundamental neurotrophic factor, and is also involved in inflammation and wound repair. PGRN may have pro- or anti-inflammatory properties, depending upon proteolysis of the anti-inflammatory parent PGRN protein and the gen...

Chouinard, D., 1989: Education at your service. L'infirmiere Auxiliaire 61(4): 16-16

Williams L.R.; Cameron G.N., 1991: Geomys attwateri. Mammalian Species (382): 1-5

Lepage, D.; Tatu, L.; Loisel, F.; Rey, P.B.; Obert, L.; Parratte, B., 2016: Anatomical and computed tomography study of the eighth costochondral junction: topography for costochondral graft harvesting. Costochondral grafts have long been used in maxillofacial reconstruction, but have been little used in trauma and orthopedic cases. This surgical technique requires that a graft be harvested from the thorax in the area of the eighth rib. Pleuropul...

Freeman, W., 1952: Prognosis in lobotomy by the Malamud rating scale. The report is based on follow-ups on 625 patients. A descr. of the scale and a patient evaluation is given. It is concluded that the syndrome most often treated by lobotomy consists in just those elements that carry the worst prognosis, namely ove...

Zanchetta G.; Vandelli L.; Mongiardo N.; Pellegrino F.; Lucchi L.; D.R.enzo B.; Savazzi A.M., 1989: Acute renal insufficiency of mononucleosis. Giornale di Malattie Infettive e Parassitarie 41(1): 82-83

Armstrong, T.J.; Chaffin, D.B., 1979: Carpal tunnel syndrome and selected personal attributes. Carpal tunnel syndrome has been reported to occur between two and ten times as frequently in women as in men. It also has been reported that this syndrome is often associated with the performance of certain manual tasks. Such cases can be consider...

Weiszer, L.; Streda, A.; Gustafik, S., 1963: The asymetry of osteophytes of the dorsal column. Revue du Rhumatisme et des Maladies Osteo-Articulaires 30: 116-119

Yang, J.; Song, Y.; Wei, W.; Ruan, L.; Ai, H., 2017: Comparison of the effectiveness of ultrasound elastography with that of conventional ultrasound for differential diagnosis of thyroid lesions with suspicious ultrasound features. Ultrasound elastography (USE) has been used for quantitative imaging since the early 1990s. The aim of the present study was to compare the effectiveness of USE with that of conventional ultrasound in the differential diagnosis of benign vs. malig...

Shiller, I.; Richmond, C.A., 1944: DDT for control of thrips on cotton. A 3% dust of DDT in pyrophyllite, when used to control Thrips tabaci and Frankliniella fusca on cotton in 1/10 acre field plots, was moderately to highly effective and more so than a sulfur dust which contained 2% dinitro-ortho-cyclohexylphenol.