+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Construction and immunogenicity of recombinant pseudorabies virus expressing the modified GP5m protein of porcine reproduction and respiratory syndrome virus

, : Construction and immunogenicity of recombinant pseudorabies virus expressing the modified GP5m protein of porcine reproduction and respiratory syndrome virus. Sheng Wu Gong Cheng Xue Bao 21(6): 858-864

Pseudorabies virus (PRV), an alpha-herpesvirus, has been used as a vector for live-viral animal vaccines. The recombinant PRV TK- / gE- / GP5+, which expressing GP5 of PRRSV, is developed based on the PRV genetic-depleting vaccine-virus strain, TK- / gE- /LacZ+. However, this strain stimulated poorly the vaccinated animals to produce neutralizing antibodies against PRRSV. In order to develop a booster specific immunized response of the PRV recombinant, the ORF5 gene of PRRSV TK- / gE- / LacZ+ was substituted by a modified ORF5 gene, ORF5m. The resultant recombinant PRV, TK- /gE- / GP5m+, was verified by PCR, Southern blotting and Western blotting. TK- / gE- / GP5m+ and TK- / gE- / GP5+ expressed GP5 proteins were inoculated into balb/c mice to evaluate their immunogenicity. The results demonstrated that the amount of neutralization antibodies and cell-immunity responses induced by TK- / gE- /GP5m+ against PRRSV were higher than that of TK- / gE- / GP5+. This study indicated that the new recombinant PRV expressing the modified GP5m protein is a candidate for the development of bivalent genetic engineering vaccines against PRRSV and PRV.


PMID: 16468337

Other references

Gottschalk, E.; Genssler, W.; Weinmann, G.; Friedrich, U., 1980: Malignant non-Hodgkin's intestinal lymphomas in children. Zentralblatt für Chirurgie 105(19): 1287-1294

Brunswick, M.; Finkelman, F.D.; Highet, P.F.; Inman, J.K.; Dintzis, H.M.; Mond, J.J., 1988: Picogram quantities of anti-Ig antibodies coupled to dextran induce B cell proliferation. To investigate the properties which enable type 2 Ag, as exemplified by dextran and Ficoll, to stimulate high levels of antibody responses in the relative absence of T cells, we conjugated anti-IgD and anti-IgM mAb to both dextran and Ficoll and e...

Gesheva V.Y.; Gesheva R.L.; Ivanova V.B.; Panayotov P.R., 1988: Taxonomy of streptomyces hygroscopicus 15 producer of an antibiotic complex. Dokladi na B"lgarskata Akademiya na Naukite 41(3): 53-56

Wilcox, L.S.; Martinez-Schnell, B.; Peterson, H.B.; Ware, J.H.; Hughes, J.M., 1992: Menstrual function after tubal sterilization. Over 100 Million women worldwide have undergone tubal sterilization. The Collaborative Review of Sterilization (CREST) study at 12 medical centers in the US between 1978-83 examined 5070 women at 4 periodic intervals up to 5 years after the proced...

Halmy, K.; Serfözö, J., 1987: Functions of polymorphonuclear granulocytes in recurrent dermatomycoses. Dermatologische Monatschrift 173(7): 405-410

Carija, A.; Navarro, S.; Ventura, S., 2016: Data on correlation between Aβ42 structural aggregation propensity and toxicity in bacteria. Protein aggregation and amyloid formation is a hallmark of an increasing number of human disorders. Because protein aggregation is deleterious for the cell physiology and results in a decrease in overall cell fitness, it is thought that natural se...

Sachdev, H.P.; Sachdev, M.S.; Verma, L.; Sood, N.N.; Moonis, M., 1989: Electrophysiological studies of the eye, peripheral nerves and muscles in epidemic dropsy. The involvement of the neurological system in epidemic dropsy is controversial. During two outbreaks of epidemic dropsy, detailed neurological and ocular examinations and electrophysiological studies of peripheral nerves and muscles (motor nerve c...

Dahl A., 1968: Status asthmaticus treated by positive pressure respiration human. Nordisk Medicin: 1047-1049

Kubal'cic, P.N.; Gubanov, I.A., 1968: Evaluation of stock collections at the botanic garden of the All-union Institute of Medicinal Plants in respect of the content of biologically active substances. Large numbers of plants in the collections contain alkaloids, glycosides, steroidal and triterpenic saponins, coumarin precursors, essential oils or tannins, and lists are given of species which require more detailed studies in respect of these co...

Young, J.R., 1967: A new nasal decongestant in otolaryngology. Eye, Ear, Nose & Throat Monthly 46(1): 51-53