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Detection of fragmented genomic DNA by PCR-free piezoelectric sensing using a denaturation approach


, : Detection of fragmented genomic DNA by PCR-free piezoelectric sensing using a denaturation approach. Journal of the American Chemical Society 127(22): 7966-7967

Label-free and real-time DNA sequence detection in PCR-amplified DNA samples can now be achieved by different approaches. On the contrary, only few works have been reported dealing with direct sequence detection in nonamplified genomic DNA. Here, a piezoelectric biosensor for direct detection of sequences in nonamplified genomic DNA is described. The system relies on real-time and label-free detection of the hybridization reaction between an immobilized probe and the complementary sequence in solution. The DNA probe is immobilized on the sensing surface (10 MHz quartz crystals), while the complementary sequence is present in the genomic DNA, previously fragmented with restriction enzymes.

US$19.90

PMID: 15926792

DOI: 10.1021/ja051345q


Other references

Auricchio F.; Migliaccio A.; D.D.menico M.; Nola E., 1987: Estradiol stimulates tyrosine phosphorylation and hormone binding activity of its own receptor in a cell free system. Recent experiments have shown that calf uterus oestrogen receptor exists in a tyrosine-phosphorylated hormone binding form and in non-phosphorylated, non-hormone binding form. We report here that physiological concentrations of oestradiol in compl...

Yang, R.; Sheng, Y.; Cao, K.; Kong, X.; Xu, D.; Yong, Y.; Zhou, L.; Zhang, H.; Qian, L.; Sun, W.; Gu, Z., 2011: Transcatheter closure of perimembranous ventricular septal defect in children: safety and efficiency with symmetric and asymmetric occluders. This study was designed to determine the safety and efficiency of asymmetric and symmetric ventricular septal occluders (AVSDOs and SVSDOs, respectively) for closure perimembranous ventricular septal defect (PMVSD) in children. Between January 200...

Leblanc, J.; Muller, A.; David, M F., 1980: Caracteristiques de l'hemagglutinine a specificite anti-A d'Helix pomatia et son utilisation pour le groupage sanguin humain. Haliotis 10(1): 61-64

Jochimsen F.; Schaefer J H.; Keller F.; Wegscheider K.; Distler A., 1991: Relevance of acute renal failure in medical intensive care a multivariate analysis. Etiology and prognosis of acute renal failure (ARF) in medical intensive-care patients were examined prospectively with linear discriminant analysis. In 261 patients, eight variable predicting the necessity for later dialysis (=risk factors) were...

Schutzler, G., 1941: Tetany in Brood Mares. After a review of cases of post-parturient tetany in brood mares described in German literature, an account is given of two cases.

Linnstaedt, S.D.; Bortsov, A.V.; Soward, A.C.; Swor, R.; Peak, D.A.; Jones, J.; Rathlev, N.; Lee, D.C.; Domeier, R.; Hendry, P.L.; McLean, S.A., 2016: CRHBP polymorphisms predict chronic pain development following motor vehicle collision. Musculoskeletal pain (MSP) is a common sequela of traumatic stress exposure. While biological factors contributing to chronic MSP after motor vehicle collision (MVC) have traditionally focused on tissue injury, increasing evidence suggests that ne...

Friedman, PA., 1979: The molecular mechanism of action of benzimidazole drugs in embryos of Ascaris suum. Dissertation Abstracts International B Sciences and Engineering, 406: 2459

Plaxton, W.C., 2003: Phosphite blocks phosphate sensing in plants and yeast. Molecular & Cellular Proteomics 2(9): 682, September

Chou, C.Hung.; Chen, P-Jer.; Jeng, Y-Ming.; Cheng, A-Lii.; Huang, L-Rung.; Cheng, J.Chia-Hsien., 2009: Synergistic effect of radiation and interleukin-6 on hepatitis B virus reactivation in liver through STAT3 signaling pathway. Hepatitis B virus (HBV) reactivation can occur after radiotherapy (RT) for hepatobiliary malignancies. Our previous in vitro culture study identified interleukin-6 (IL-6) as the main bystander mediator of RT-induced HBV replication. We attempted t...

Duffek, J., 1990: Modification of cucumber forcing on straw using nutrient solution. The results of greenhouse trials with cultivars Sandra F1, Saladin F1 and Pollex F1 grown on wheat straw bales are presented. No marked benefit was produced by placing 50-mm-thick polystyrene mats under the straw or by covering the straw (after pl...