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Determination of steroids in milk powder and birds' feces by high performance liquid chromatography coupled with evaporative light scattering detector

, : Determination of steroids in milk powder and birds' feces by high performance liquid chromatography coupled with evaporative light scattering detector. Se Pu 15(2): 102-105

In this paper, a new, simple and rapid method for the analysis of steroids by reversed-phase high performance liquid chromatography (HPLC) coupled with evaporative light scattering detector (ELSD) is presented. A good resolution of the 12 steroids is obtained within 35 min with a gradient elution using a binary mobile phase composed of methanol and water. The analytical column used was 2.0mm x 150mm, packed with BDS (particle size 5microm) and the mobile phase flow rate was 0.2mL/min. Nebulizer gas flow rate and drift tube temperature, the two critical parameters for determining the reproducibility and signal-to-noise ratio of ELSD were 2.00mL/min and 95 degrees C, respectively. The response of the ELSD was non-linear as a result of basic light-scattering principles, the calibration curve by plotting lnY vs lnX (Y, X stand for peak area and concentration, respectively) shows a good linearity in the range of 5-50mg/L with a correlation coefficient higher than 0.99 for individual steroid and with relative standard deviation less than 6.92%. This method has been applied to the determination of steroids in milk powder and birds' feces, solid phase extraction on reverse phase bonded C18 (5mL/500mg) was used to treat the samples efficiently. The recoveries of the steroids were between 84.0%-102.8%.


PMID: 15739391

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Lee, S.S.; Humphreys, K.L., 2014: Interactive association of dopamine receptor (DRD4) genotype and ADHD on alcohol expectancies in children. Positive and negative alcohol expectancies (AEs) are beliefs about the consequences of alcohol use (e.g., happy, sad, lazy) and they predict patterns of adolescent and adult alcohol engagement in clinical and nonclinical samples. However, signific...

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van Nes, F.L., 1968: Enhanced visibility by regular motion of retinal images. American Journal of Psychology 81(3): 367-374

Irons, D.W.; Bullough, C.H.W., 1993: Hyperextension of the head caused by a fetus papyraceus in a monochorionic twin pregnancy. Journal of Obstetrics & Gynaecology (Abingdon) 13(6): 451-452

Nahas, R.I.; Lever, J.R.; Lever, S.Z., 2007: Synthesis and structure-activity relationships of N-(3-phenylpropyl)-N'-benzylpiperazines: Potent ligands for sigma1 and sigma2 receptors. Ten N-(3-phenylpropyl)-N'-benzylpiperazines having different substituents on the benzyl moiety were synthesized and evaluated for sigma(1) and sigma(2) receptor binding. The sigma(1) affinities were 0.37-2.80nM, sigma(2) affinities were 1.03-...

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