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Generation of novel conditional and hypomorphic alleles of the Smad2 gene


, : Generation of novel conditional and hypomorphic alleles of the Smad2 gene. Genesis 40(2): 118-123

Smad2 is an intracellular mediator of the transforming growth factor beta signaling (TGFbeta) pathway. It has been previously shown that, in the mouse, ablation of functional Smad2 results in embryonic lethality due to gastrulation defects. To circumvent the early lethality and study the spatially and temporally specific functions of Smad2, we utilized the Cre-loxP system to generate a Smad2 conditional allele. Here we show that a conditional allele, Smad2(flox), was generated. In this allele, exons 9 and 10 are flanked by loxP sites and the gene is functionally wildtype. Cre-mediated recombination results in a deletion allele which phenocopies our previously reported Smad2(DeltaC) null mutation. To generate this conditional allele, we first made a targeted mutation which introduced a floxed neo cassette into intron 10. This allele (Smad2(3loxP)) functions hypomorphically when placed opposite a null allele, and unlike the other published Smad2 hypomorphic allele, can be maintained in the homozygous state.

US$19.90

PMID: 15452874

DOI: 10.1002/gene.20072


Other references

Smith Lee Anderson, 1985: Biostratigraphy of the stratotype Pleistocene section at Vrica, Italy. TER QUA Symposium Series Institute for Tertiary Quaternary Studies: ges 67-69. 1985.

Goldenberg, D.; Begum, S.; Westra, W.H.; Khan, Z.; Sciubba, J.; Pai, S.I.; Califano, J.A.; Tufano, R.P.; Koch, W.M., 2008: Cystic lymph node metastasis in patients with head and neck cancer: An HPV-associated phenomenon. Background. Cystic lymph node metastases have been associated with tonsil cancer. A subset of oropharyngeal cancers contain human papillomavirus (HPV) DNA. The clinical and virologic associations of cystic nodal metastasis in head and neck cancer...

Vermylen, J., 1970: Blood coagulation problems in the surgical patient. Acta Chirurgica Belgica Suppl 1: 87-92

Willcox, R.R., 1952: The terramycin treatment of gonorrhoea with two grammes over forty-eight hours. South African Medical Journal 26(34): 688-689

Vessey, D.A.; Kelley, M.; Lau, E.; Zhang, S.Z., 2000: Monovalent cation effects on the activity of the xenobiotic/medium-chain fatty acid:CoA ligases are substrate specific. The effect of monovalent cation on the activity of the XL-I and XL-III forms of xenobiotic/medium-chain fatty acid:CoA ligase (XM-ligase) was investigated using a variety of different carboxylic acid substrates. With benzoate or p-hydroxybenzoate...

Loomis C.W.; Arunachalam K.D.; Vyas D.; Milne B., 1992: Potentiation of intrathecal it dexmedetomidine antinociception by it methoxamine in the rat evidence for an endogenous opioid link. Canadian Journal of Anaesthesia 39(5 PART 2): A13

Melidoro G., 1967: Quaternary gravitational movements of the scaled ophiolitic clays and landslides produced in the Pleistocene sediments near Cassano al Ionio. Landslides caused by the Cretaceous-middle Eocene scaled ophiolitic clays, Italy.

Tayib, A.; Mosli, H.; Atwa, M., 2006: Cowper's syringocele (cyst of the bulbar urethra): a case report and literature review. Annals of Saudi Medicine 23(3-4): 194-195

Wenter, T., 2013: What's going on with Medicaid?. Ohio Nurses Review 88(4): 14-16

Olofsson, Johan, 2006: Short- and long-term effects of changes in reindeer grazing pressure on tundra heath vegetation. Previous studies of plant-herbivore interactions have typically focused either on short-term or long-term effects. By directly comparing effects at different temporal scales, I studied whether short-term experiments provide good indications of the...