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Hybrid Capture 2 vs HPV Consensus kit: comparison of 2 assays for human papillomavirus detection and typing


, : Hybrid Capture 2 vs HPV Consensus kit: comparison of 2 assays for human papillomavirus detection and typing. Pathologie-Biologie 53(8-9): 556-562

Many laboratories use the DNA Hybrid Capture 2 HPV-high risk assay (Digene) to detect and type oncogenic HPV. The aim of this work was to compare this assay with a new HPV genotyping assay: HPV Consensus kit (Argène). Actually, this assay is not commercially available. Ninety-four cervical samples were tested with both the routine assay Hybrid Capture 2 and the HPV Consensus kit. Discordant results were analysed by amplification with a nested PCR and sequencing of amplified products. Only 81 results could be analysed concerning the oncogenic risk. The overall concordance was 92,6%. But we find 13 "generic" results with the HPV Consensus kit, the generic probe including high risk and low risk genotypes. HPV Consensus kit results showed a better detection sensitivity for this assay than Hybrid Capture 2 assay. Nevertheless, "generic" results give no information about the oncogenic risk of the HPV detected in a sample.

US$19.90

PMID: 16176861

DOI: 10.1016/j.patbio.2005.07.005


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Biglioli, F.; Colombo, V.; Tarabbia, F.; Autelitano, L.; Rabbiosi, D.; Colletti, G.; Giovanditto, F.; Battista, V.; Frigerio, A., 2012: Recovery of emotional smiling function in free-flap facial reanimation. Long-standing unilateral facial palsy is treated primarily with free-flap surgery using the masseteric or contralateral facial nerve as a motor source. The use of a gracilis muscle flap innervated by the masseteric nerve restores the smiling funct...

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Binns, E.S., 1981: Fungus gnats (Diptera:Mycetophilidae/Sciaridae) and the role of mycophagy in soil: a review. The abundance of soil-dwelling fungus-gnats and their role in the primary decomposition of litter is examined and the nature of their mycophagy is discussed. Sciarid fungus-gnats are adapted to exploit fungal growth associated with litter but this...

Goldman, I.L.; Breitbach, D.N., 1996: Inheritance of a recessive character controlling reduced carotenoid pigmentation in carrot (Daucus carota L.). All previously identified white or nonpigmented carrot roots such as those from wild carrot and white derivatives of yellow or orange types have been dominant to the production of pigment, which is recessive. The primary pigments in orange carrot...

Unsgaard, G.; Ommedal, S.; Rygh, O.M.; Lindseth, F., 2005: Operation of arteriovenous malformations assisted by stereoscopic navigation-controlled display of preoperative magnetic resonance angiography and intraoperative ultrasound angiography. To study the application of navigated stereoscopic display of preoperative three-dimensional (3-D) magnetic resonance angiography and intraoperative 3-D ultrasound angiography in a clinical setting. Preoperative magnetic resonance angiography and...

El-Tayeb, T.S.; Abdelhafez, A.A.; Ali, S.H.; Ramadan, E.M., 2013: Effect of acid hydrolysis and fungal biotreatment on agro-industrial wastes for obtainment of free sugars for bioethanol production. This study was designed to evaluate selected chemical and microbiological treatments for the conversion of certain local agro-industrial wastes (rice straw, corn stalks, sawdust, sugar beet waste and sugarcane bagasse) to ethanol. The chemical com...

Wilkins, KT., 1989: Tabarida brasiliensis. Mammalian Species, 1-10 No. 331

Pekkarinen H.A.; Finne A.M.; Mahlamaki S.T.; Hanninen O.O.P., 1989: Motor fitness and its relation to body dimensions and growth in young male cross country skiers and controls. A total of 173 Finnish boys, comprising 94 cross-country skiers and 79 controls, who were in turn divided into two groups, were studied during three successive years. In the beginning of the study the boys were 10 to 14 years old. The young male s...

Kofler, E., 1952: Granulomata of the gastrointestinal tract. Virchows Archiv für Pathologische Anatomie und Physiologie und für Klinische Medizin 321(2): 121-133