geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Identification of epithelial cell adhesion molecule autoantibody in patients with ovarian cancer


, : Identification of epithelial cell adhesion molecule autoantibody in patients with ovarian cancer. Clinical Cancer Research 9(13): 4782-4791

The epithelial cell adhesion molecule (Ep-CAM) exhibited an ovarian cancer:normal human ovarian surface epithelium ratio of 444. For validation studies, real-time quantitative PCR analysis and immunohistochemistry were performed in normal and malignant ovarian epithelial cell lines and tissues. To evaluate the potential of the Ep-CAM autoantibody as a tumor marker, we examined the amount of Ep-CAM autoantibody in serum samples obtained from ovarian cancer patients and normal controls by an ELISA. Real-time quantitative PCR analysis revealed significant overexpression of Ep-CAM mRNA in cancer cell lines (P < 0.001) and microdissected cancer tissues (P < 0.05), compared with that in cultured normal human ovarian surface epithelium and microdissected germinal epithelium, respectively. Immunolocalization of the Ep-CAM autoantibody showed that the sera of ovarian cancer patients expressed higher levels of Ep-CAM autoantibody than benign tumor patients and normal controls (P < 0.05). The levels of Ep-CAM autoantibody found were as follows: 0.132 in 52 patients with ovarian cancer, 0.098 in 26 cases with benign gynecologic disease, and 0.090 in 26 normal women. This investigation has shown that the Ep-CAM autoantibody was found to be associated with ovarian cancer and suggested that future research assessing its clinical usefulness would be worthwhile.

US$29.90

PMID: 14581349


Other references

Anonymous, 1993: Clinical Pharmacology of Manidipine for the Treatment of Hypertension: Importance of Renal and Additional Organ Protective Properties. Proceedings of a symposium. Madrid, Spain, June 13, 1992. American Heart Journal 125(2 Pt 2): 559-648

Gaddipati J., 1984: Phase i ii trial of amsacrine with high dose cytarabine in the treatment of acute leukemia. Cancer Immunology Immunotherapyppl: S3

Ledikwe, J.H.; Kejelepula, M.; Maupo, K.; Sebetso, S.; Thekiso, M.; Smith, M.; Mbayi, B.; Houghton, N.; Thankane, K.; O'Malley, G.; Semo, B-Werq., 2013: Evaluation of a well-established task-shifting initiative: the lay counselor cadre in Botswana. Evidence supports the implementation of task shifting to address health worker shortages that are common in resource-limited settings. However, there is need to learn from established programs to identify ways to achieve the strongest, most sustai...

Scheding, S.; Media, J.E.; Nakeff, A., 1994: Acute toxic effects of 3'-azido-3'-deoxythymidine (AZT) on normal and regenerating murine hematopoiesis. The immediate hematopoietic response to 3'-azido-3'-deoxythymidine (AZT) was characterized in an unperturbed and regenerating mouse marrow model to identify the in vivo hematopoietic targets of AZT and test whether AZT toxicity is depend...

Saeki, M.; Saito, Y.; Sai, K.; Maekawa, K.; Kaniwa, N.; Sawada, J-ichi.; Kawamoto, M.; Saito, A.; Kamatani, N., 2007: A combinatorial haplotype of the UDP-glucuronosyltransferase 1A1 gene (#60-#IB) increases total bilirubin concentrations in Japanese volunteers. Clinical Chemistry 53(2): 356-358

Getachew, Y.; Cusimano, F.A.; James, L.P.; Thiele, D.L., 2015: The role of intrahepatic CD3+/CD4-/CD8- double negative T (DN T) cells in enhanced acetaminophen toxicity. The role of the immune system, specifically NK, NKT and CD3 cells, in acetaminophen (APAP) induced liver injury remains inconsistently defined. In the present study, wild type (C57BL/6J) mice and granzyme B deficient (GrB -/-) mice were treated wi...

Goss, P.E., 1998: Pre-clinical and clinical review of vorozole, a new third generation aromatase inhibitor. Vorozole (Rivizor), is a triazole derivative and one of the new, third generation aromatase inhibitors. Vorozole causes reversible inhibition of cytochrome P450 aromatase with the majority of the aromatase inhibition activity attributable to the d...

Doh, K-Oh.; Kim, Y-Woon.; Park, S-Young.; Lee, S-Kang.; Park, J.Sik.; Kim, J-Yeon., 2005: Interrelation between long-chain fatty acid oxidation rate and carnitine palmitoyltransferase 1 activity with different isoforms in rat tissues. This study examined the interrelation between the long-chain fatty acid (LCFA) oxidation rate and the carnitine palmitoyltransferase (CPT) 1 activity in various tissues containing L-CPT1 or M-CPT1. The Liver, kidney, heart, white and red gastrocne...

Kirk-Spriggs, AH., 1994: Two name changes to the British list of Meligethes Stephens, 1830 (Nitidulidae). Coleopterist. May; 31: 9

Keung, A.; Eller, M.G.; McKenzie, K.A.; Weir, S.J., 1999: Single and multiple dose pharmacokinetics of rifapentine in man: part II. To characterize the pharmacokinetics of rifapentine following single, multiple, and intermittent doses. Twenty-three healthy male volunteers were randomized in a two-period, incomplete block, crossover design to receive two of four possible treatm...